[Study of the effects of gamma-irradiation of common wheat F1 seeds using gliadins as genetic markers].

Effects of irradiation of dry F1 seeds with gamma rays in the dose of 200 Gy were studied. Hybrids between near-isogenic lines on the basis of the variety Bezostaya 1 served as the material of investigation. Irradiation markedly reduced productivity traits of F1 plants and did not affect the survival of F1 plants under the given growth conditions. A significant relative increase in the frequency of pollen grains with the 1BL/1RS translocation that formed F2 seeds in comparison with the control was one of the effects of irradiation of F1 seeds. Irradiation with gamma-rays induced mutations at gliadin loci with the frequency of 7,4 % (at 0,5 % in the control).

[Genetically modified plants and the problems of plant protection: progress and estimation of potential risks].

The review deals with advances and prospects in development of transgenic plants. At present virtually all commercial GM crops are those created for solving plant protection problems--they carry transgenes conferring resistance to herbicides, pests, viruses. Approaches employed for development of commercial GM crops with herbicide, pest and virus resistance, as well as strategies and prospects of development of commercial GM plants with resistance to fungal and bacterial diseases and nematodes, are considered. Ecological (including agronomical) and social risks associated with commercial growing of transgenic plants are briefly discussed.

[Identification of 1AL/1RS translocation in winter common wheat varieties of Ukrainian selection].

Analysis of alcohol-soluble protein (gliadin) patterns of a group of winter common wheat varieties from different breeding centers of Ukraine resulted in identification of six varieties with the rye 1AL/1RS translocation. The line 7086 AR also carries this translocation. The identified 1AL/1RS translocation is similar to that of the variety Amigo in the secalin pattern. The appearance of this translocation in varieties developed in different breeding centers of the forest-steppe of Ukraine indicates its involvement in coadaptive gene associations.

[Allele characterization of storage protein loci in the Greek spring wheat cultivars]. / Alel'nyi sklad za lokusamy zapasnykh bilkiv hrupy hrets'kykh sortiv iaroï m'iakoï pshenytsi.

Genotypes at the storage protein loci Glu-A1, Glu-B1, Glu-D1, Gli-A1, Gli-B1, Gli-D1, Gli-A3 were identified in a group of Greek spring common wheat varieties. These varieties served as the parental forms for producing dihaploid lines. Heterogeneous varieties were revealed.

[A comparative phylogenetic analysis of the coding portion of the genes for structural protein E1 in the Shi-Min' strain and in other strains of the classic hog cholera virus]. / Porivnial'nyi filohenetychnyi analiz koduiuchoï chastyny heniv strukturnoho bilka E1 shtamu Shi-Myn' ta inshykh shtamiv virusu klasychnoï chumy svynei.

The primary nucleotide sequence of the fragment of E1 (gp51-55) gene (772 bp in length) of virulent strain Shi-Min' of classical swine fever virus (CSFV) has been determined. Multiple alignments of the E1 gene fragments of various strains and isolates of CSFV, which are homologues to the cloned fragment, has been carried out; the phylogenetic tree has been plotted and the consensus sequence has been determined. The strain Shi-Min', which was used as a control CSFV strain in former Soviet republics, and the referent strain Alfort were shown to be located on the opposite sides of the dendrogram, while Shi-Min' was more close to the western European group of CSFVs. We suggest that Shi-Min' strain as a positive viral antigen for vaccine testing represents a selective factor for the formation of virus population in Ukraine. The data obtained are the first results in molecular epizootic study of hog cholera in Ukraine.