Intestinal gene expression profiles of piglets benefit from maternal supplementation with a yeast mannan-rich fraction during gestation and lactation.

The objective was to study the effect of maternal supplementation with a yeast cell wall-based product containing a mannan-rich fraction (MRF) during gestation and lactation on piglet intestinal gene expression. First parity sows were fed experimental gestation and lactation diets with or without MRF (900 mg/kg). After farrowing, piglets were fostered within treatment, as necessary. Sow and litter production performance data were collected until weaning. On day 10 post farrowing, jejunum samples from piglets were collected for gene expression analysis using the Affymetrix Porcine GeneChip array. Most performance parameters did not differ between the treatments. However, protein (P<0.01), total solids less fat (P<0.03) and the concentration of immunoglobulin G (IgG) in milk were greater (P<0.05) in the MRF-supplemented group. Gene expression results using hierarchical clustering revealed an overall dietary effect. Further analysis elucidated activation of pathways involved in tissue development, functioning and immunity, as well as greater cell proliferation and less migration of cells in the jejunum tissue. In conclusion, feeding the sow MRF during pregnancy and lactation was an effective nutritional strategy to bolster colostrum and milk IgG that are essential for development of piglet immune system and gut. In addition, the gene expression patterns affected by the passive immunity transfer showed indicators that could benefit animal performance long term.

Effects of yeast cell wall-derived mannan-oligosaccharides on jejunal gene expression in young broiler chickens.

The use of mannan-oligosaccharides (MOS) as alternatives to antibiotic growth promoters (AGP) has gained in popularity in recent years due to regulatory restrictions of using AGP in food animal production. Benefits of MOS usage include improvement on animal performance, feed efficiency, and gastrointestinal health. The molecular mechanisms of these functions however are not clear. The goal of the current study was to use a transcriptomics approach to investigate the effects of MOS on the intestinal gene expression profile of young broilers and characterize biological gene pathways responsible for the actions of MOS. One hundred and twenty 1-d-old Cobb 500 broiler chicks were randomly divided into 2 groups and were fed either a standard wheat-soybean meal-based (control) diet or the same diet supplemented with 2.2 g/kg of MOS (Bio-Mos, Alltech, Nicholasville, KY) for 3 wk, followed by jejunal gene expression profiling analysis using chicken-specific Affymetrix microarrays. Results indicated that a total of 672 genes were differentially expressed (P < 0.01 and fold change >1.2) in the jejunum by MOS supplementation. Association analysis indicated that differentially expressed genes are involved in diverse biological functions including energy production, cell death, and protein translation. Expression of 77 protein synthesis-related genes was differentially regulated by MOS in the jejunum. Further pathway analysis indicated that 15 genes related to oxidative phosphorylation were upregulated in the jejunum, and expression of genes important in cellular stress response, such as peroxiredoxin 1, superoxide dismutase 1, and thioredoxin, were also increased by MOS. Differential expression of genes associated with cellular immune processes, including lysozyme, lumican, ß 2-microglobin, apolipoprotein A-1, and fibronectin 1, were also observed in MOS-fed broilers. In summary, this study systematically identified biological functions and gene pathways that are important in mediating the biological effects of MOS in broilers.

Utility of automated data in identifying femoral shaft and subtrochanteric (diaphyseal) fractures.

UNLABELLED: In women age 45 years and older, enrolled in an integrated group practice in 2007, use of ICD9 diagnostic codes, including the "not otherwise specified" code (821.00) resulted in a high false-positive rate for identifying femoral diaphyseal fractures. Restriction to more specific site-codes missed 36% of these rare fractures. INTRODUCTION: The aim of this study was to assess the utility of automated data in identifying the occurrence of femoral diaphyseal fractures. METHODS: We identified all women age 45 years and older enrolled in a Pacific Northwest integrated group practice during 2007. Using the computerized database we selected all ICD9 codes that could be related to a femur fracture occurring in the diaphyseal region. We then quantified the percent of codes confirmed by medical record review to have occurred in the correct anatomic location during the year of interest (positive predictive value). RESULTS: Of the 95,765 eligible women, 161 (0.17%) had an ICD9 diagnostic code potentially related to a femoral diaphyseal fracture in 2007; of these 58 (36%) had a fracture of the femoral diaphysis, and 38 (24%) of the fractures occurred in 2007. The most frequent code was 821.00, described as "femur fracture not otherwise specified", applied to 107 women; 21 of the 58 diaphyseal fractures had this code. CONCLUSION: In this study, use of ICD9 codes that included the "not otherwise specified" code (821.00) resulted in a high false-positive rate for identifying diaphyseal fractures. However, restriction to more specific site codes would have missed at least 36% of the diaphyseal fractures. Furthermore, the codes did not provide any information about the characteristics of the fracture. Our findings support validating cases selected using ICD codes before they are used as a surrogate for the occurrence of femoral diaphyseal fractures.

TFIIH action in transcription initiation and promoter escape requires distinct regions of downstream promoter DNA.

TFIIH is a multifunctional RNA polymerase II general initiation factor that includes two DNA helicases encoded by the Xeroderma pigmentosum complementation group B (XPB) and D (XPD) genes and a cyclin-dependent protein kinase encoded by the CDK7 gene. Previous studies have shown that the TFIIH XPB DNA helicase plays critical roles not only in transcription initiation, where it catalyzes ATP-dependent formation of the open complex, but also in efficient promoter escape, where it suppresses arrest of very early RNA polymerase II elongation intermediates. In this report, we present evidence that ATP-dependent TFIIH action in transcription initiation and promoter escape requires distinct regions of the DNA template; these regions are well separated from the promoter region unwound by the XPB DNA helicase and extend, respectively, approximately 23-39 and approximately 39-50 bp downstream from the transcriptional start site. Taken together, our findings bring to light a role for promoter DNA in TFIIH action and are consistent with the model that TFIIH translocates along promoter DNA ahead of the RNA polymerase II elongation complex until polymerase has escaped the promoter.

Assignment of CDC weak oxidizer group 2 (WO-2) to the genus Pandoraea and characterization of three new Pandoraea genomospecies.

CDC weak oxidizer group 2 (WO-2) consists of nine phenotypically similar human clinical isolates received by the Centers for Disease Control and Prevention between 1989 and 1998. Four of the isolates were from blood, three were from sputum, and one each was from bronchial fluid and maxillary sinus. All are aerobic nonfermentative, motile gram-negative rods with one to eight polar flagella per cell. All grew at 25 and 35 degrees C and were positive for catalase, urease (usually delayed 3 to 7 days), citrate, alkalinization of litmus milk, oxidization of glycerol (weakly), and growth on MacConkey agar and in nutrient broth without NaCl. All except one strain were oxidase positive with the Kovács method, and all except one isolate weakly oxidized D-glucose. All were negative for oxidation of D-xylose, D-mannitol, lactose, sucrose, maltose, and 20 other carbohydrates, esculin hydrolysis, indole production, arginine dihydrolase, and lysine and ornithine decarboxylase. Only two of nine isolates reduced nitrate. Broth microdilution susceptibilities were determined for all strains against 13 antimicrobial agents. Most of the strains were resistant to ampicillin, extended-spectrum cephalosporins, and aminoglycosides, including gentamicin, tobramycin, and amikacin, but they varied in their susceptibility to fluoroquinolones. High-performance liquid chromatographic and mass spectrometric analyses of the WO-2 group identified ubiquinone-8 as the major quinone component. The percent G+C of the WO-2 strains ranged from 65.2 to 70.7% (thermal denaturation method). All shared a common cellular fatty acid (CFA) profile, which was characterized by relatively large amounts (7 to 22%) of 16:1omega7c, 16:0, 17:0cyc, 18:1omega7c, and 19:0cyc(11-12); small amounts (1 to 3%) of 12:0 and 14:0; and eight hydroxy acids, 2-OH-12:0 (4%), 2-OH-14:0 (trace), 3-OH-14:0 (12%), 2-OH-16:1 (1%), 2-OH-16:0 (3%), 3-OH-16:0 (4%), 2-OH-18:1 (2%), and 2-OH-19:0cyc (3%). This profile is similar to the CFA profile of Pandoraea, a recently described genus associated with respiratory infections in cystic fibrosis patients (T. Coenye et al., Int. J. Syst. Evol. Microbiol., 50:887-899, 2000). Sequencing of the 16S rRNA gene (1,300 bp) for all nine strains indicated a high level (> or =98.8%) of homogeneity with Pandoraea spp. type strains. DNA-DNA hybridization analysis (hydroxyapatite method; 70 degrees C) confirmed the identity of WO-2 with the genus Pandoraea and assigned three strains to Pandoraea apista and three to Pandoraea pnomenusa, and identified three additional new genomospecies containing one strain each (ATCC BAA-108, ATCC BAA-109, ATCC BAA-110). This study also shows that Pandoraea isolates may be encountered in blood cultures from patients without cystic fibrosis.

Biomechanical factors and failure of transcervical hip fracture repair.

OBJECTIVE: To assess the association between biomechanical measurements (bone quality of the femoral neck, comminution, fracture angle, and fracture level) and the likelihood of fixation failure among patients who have a multiple screw stabilisation of an intracapsular hip fracture. METHODS: A cohort study of 139 Washington State residents greater than 60 years of age who sustained a fall-related transcervical hip fracture treated from 1990 to 1996 inclusive. Measurements of bone quality, fracture angle, fracture level, and comminution were taken from perioperative X-rays. The outcome measure was clinical failure of the internal fixation procedure within 12 months of hospital discharge, as measured by readmission for further surgery to that hip. RESULTS: Of the four biomechanical aspects examined, only bone quality, as measured by presence of an ICD code for osteoporosis, was significantly associated with risk of subsequent hospitalisation for revision surgery (adjusted hazard ratio 7.7, 95% CI 1.8-32.8). CONCLUSION: A diagnosis code for osteoporosis was related to the outcome of intracapsular fractures repaired with multiple pins. Other biomechanical measurements from diagnostic X-rays were not related to the need for further surgery.

Synthesis and biological activity of enantiomeric pairs of phosphosulfonate herbicides.

The phosphosulfonates are a new class of soil-active herbicides which control a variety of annual grass and broadleaf weeds. Chirality at the phosphorus atom afforded the opportunity to explore stereospecific requirements for herbicidal activity. Chiral (hydroxymethyl)phosphinate intermediates were enzymatically resolved (Pseudomonas fluorescens lipase) from the racemic mixtures and then used to prepare two pairs of enantiomeric phosphosulfonates. Biological testing of the enantiomeric phosphosulfonate herbicides demonstrated that, in each case, the herbicidal activity was attributed to the (+) enantiomer and that the (+) enantiomer is more active than the racemate.

Risk factors for the between-herd spread of Mycobacterium bovis in Canadian cattle and cervids between 1985 and 1994.

Microorganisms of the genus Mycobacterium cause tuberculosis in many animal species including humans. Generally, Mycobacterium bovis (M. bovis) infects cattle and cervids, but it has the potential to infect virtually all species of mammals. This study examined and analysed the data from the nine outbreaks of tuberculosis in Canadian cattle and cervids from 1985 to 1994. For the purposes of this study, a positive herd was one with at least one culture-positive animal. A reactor herd had at least one animal which was positive or suspicious on a mid-cervical, comparative cervical, or gross or histopathologic test for tuberculosis. Herd classification was either reactor/positive or negative. Data for the study were collected from the outbreak records in the Regional or District offices of Agriculture and Agri-Food Canada. Logistic regression was used to study spread of tuberculosis between herds. Two risk factors were identified: increasing herd size; and, the reason why a herd was investigated as part of the outbreak. This latter factor was interpreted as a surrogate measure for the nature of contact between the study herd and other potentially infected herds in the outbreak. Increasing herd size was associated with an increased risk of being positive for tuberculosis with herds of 16-35, 36-80, and >80 animals having odds ratios of 2.9, 5.8, and 9.3, respectively, when compared to a herd size of <16 animals (p < 0.001). When compared to perimeter testing (i.e. testing herds within a specified radius of an infected herd), all other reasons for investigation had higher odds ratios (p < 0.001). These odds ratios were 57.8 for traceout herds (i.e. herds which had purchased animal(s) from a reactor/positive herd), 31.8 for herds with pasture or fence-line contact with a reactor/positive herd, and 14.9 for traceback herds (i.e. herds which had been a source of animals for reactor/positive herd(s)).

Structural information from methyl internal rotation spectroscopy.

The fundamental quantum mechanics, group theory, and spectroscopy of methyl torsional structure accompanying electronic transitions is presented. The origin of barriers to internal rotation and the interaction of the methyl with the pi system via hyperconjugation are discussed. Because of the relationship between the methyl barrier and the pi system, measurement of the CH3 properties provides structural information about the molecule. In para'-substituted p-methyl-t-stilbenes, barriers in the S1 state show a strong dependence on the substituent, substituent conformation, and involvement of the substituent in hydrogen bonding interaction. The methyl torsional barrier reflects these changes despite the distance of the substitution site, 10 atoms away.

The transition to home for mothers of healthy and initially ill newborn babies.

Mothers' difficulties with the transition of their baby from hospital to home have been studied for several years. An examination of a number of studies from the USA, Canada, and the UK has shown a remarkable similarity in the findings despite the demographic and cultural differences in the sample characteristics. It appears, then, that maternal responses to the transition to home are more tied to the experience than to the setting. The purpose of this paper is to place these findings within a framework that would provide a means for midwives and nurses to help mothers make this transition more comfortably and confidently than in the past. The framework chosen is the Transition Model as developed by Kenner (1988). This model suggests the problems with transition to home can be addressed under the following categories: 1. information needs; 2. grief; 3. parent-child development; 4. stress and coping; 5. social support.

Prevalence of feline immunodeficiency virus in submissions of feline serum to a diagnostic laboratory in Atlantic Canada.

The purpose of this project was to identify the prevalence of feline immunodeficiency virus (FIV) in the Atlantic region of Canada, and to determine possible associations between FIV serological status and breed, sex, and age. Feline serum samples (671) submitted to the Prince Edward Island Diagnostic Services - Atlantic Veterinary College laboratory between January 1, 1988 and July 30, 1989 were considered eligible for this study. The majority of samples originated from Prince Edward Island (607). Testing was performed in duplicate using commercial 96-well enzyme-linked immunosorbent assay test kits for FIV antibody. Results included a seropositive rate of 7.6% for all submissions. Mean age of FIV-seropositive cats was eight years. There was an increasing risk of FIV-seropositive status associated with age. Prevalence of FIV among intact males was significantly higher (odds ratio = 2.59) than other gender categories. The principal conclusion of this study was that FIV is present in cats of the Atlantic provinces, and that its associations and prevalence are consistent with those found in other North American epidemiological studies.

Bovine fetal infection with Mycobacterium paratuberculosis.

In utero transmission of Mycobacterium paratuberculosis, the causative agent of paratuberculosis in cattle, has been suggested. Tissue specimens were obtained at a packing plant from pregnant dairy cows and their fetuses and from cows with clinical signs of paratuberculosis and from their fetuses. Specimens were processed according to methods described for isolating M paratuberculosis from bovine tissues and were incubated on Herrold egg yolk medium for 16 weeks. Presumed positive specimens were confirmed to be M paratuberculosis, using acid-fast staining and subculturing. Of 407 lymph nodes from cows, 34 (8.4%) were culture positive for M paratuberculosis; 9 of 34 (26.4%) of these culture-positive cows had fetuses from which specimens were also culture positive. The results estimated the risk of fetal infection with M paratuberculosis to be 26.4% (95% confidence interval between 11.3 and 40.7%).

The investigation of outbreaks of infectious disease.

The ability to control infectious diseases is contingent upon recognizing and eliminating the source of infection or disrupting the transfer of infectious agent to susceptible hosts. In this article, methods of identification of infectious agents, the presence of carriers and environmental reservoirs, and their impact upon investigations are discussed.