ABSTRACT
A strain-specific, elevated serum free glycerol has been found in stock diet fed, carbohydrate-sensitive, hyperlipemic BHE rats. This suggests that considerably more peripheral lipolysis is taking place in the nonfasting BHE rat than in its Wistar counterpart. This observation further suggests that the BHE rat may exhibit metabolic changes in the fed state that are similar to those found in normal fasting rats. Individual, serum free glycerol levels were not correlated with individual serum insulin values. In the nonfasted BHE rats, serum free glycerol concentrations were found to be sufficiently high to initiate gluconeogenesis or to contribute to a maximal rate of triglyceride synthesis.
Subject(s)
Dietary Carbohydrates/adverse effects , Glycerol/blood , Hyperinsulinism/metabolism , Hyperlipidemias/metabolism , Animals , Dietary Carbohydrates/metabolism , Fasting , Gluconeogenesis , Glycerides/blood , Insulin/blood , Lipid Metabolism , Male , Rats , Species SpecificityABSTRACT
A glucagon-saline solution (0.1 ml, 10(7) mole/100 g body weight) was injected via the portal vein into nonfasted Wistar and carbohydrate-sensitive BHE rats. Levels of liver and epididymal fat pad cyclic-AMP were observed after 6 and 24 minutes. When compared to sham injected rats at 6 minutes, glucagon injected rats of both strains had twice the level of cyclie-AMP in liver and fat pad tissue. By 24 minutes, the cyclic-AMP levels of the Wistar rats had decreased to those observed in their sham injected counterparts, and the concentration of liver cyclic-AMP in both sham injected and glucagon injected BHE rats had decreased to levels significantly below those observed in the Wistar rats. This observation suggests that a lipolytic-lipogenic imbalance may reside in the livers of rats of the BHE strain.