Adeno-associated virus may play a protective role against human papillomavirus-induced cervical lesions independent of HIV serostatus.

This study investigated the prevalence of adeno-associated virus (AAV) and human papillomavirus (HPV) DNA in cervical samples of HIV-seropositive and -seronegative women attending a clinic in south-eastern Brazil. Both viruses were investigated by polymerase chain reaction (PCR) and cytological exams were performed. AAV was typed by PCR and restriction fragment length polymorphism analysis. AAV prevalence was 19.7% (56/284), with 18.7% (21/112) and 20.3% (35/172) in HIV-positive and -negative women, respectively. AAV type 2 was the single virus type detected. AAV was detected with higher frequency in HPV-infected women (P < 0.05) as was HPV in HIV-positive women (P < 0.05). The AAV-HPV co-infected women showed a lower rate of atypical squamous cells of undetermined significance or cervical intraepithelial neoplasia development compared with those infected only with HPV. The prevalence of AAV2 confirms this type as the most common in human samples. This is the first report examining AAV in cervical samples of HIV-infected women and indicates that HIV infection does not appear to influence AAV prevalence or AAV-HPV co-infection.

Prevalence of human cytomegalovirus infection in pregnant and non-pregnant women.

OBJECTIVES: To determine human cytomegalovirus (HCMV) cervical reactivation in both pregnant and non-pregnant women and to ascertain whether or not it occurs in conjunction with hematogenic dissemination. METHODS: Clinical specimens were obtained from 40 pregnant and 62 non-pregnant women attended at the Ambulatory of the Gynecology-Obstetrics Unit of the Federal University of Espírito Santo (UFES) in Southeastern Brazil. Specimens under investigation were blood samples submitted to seroprevalence determination, antigenemia assay, HCMV-DNA detection, and vaginal secretion, submitted to HCMV-DNA detection. RESULTS: Viral seroprevalence was found in 98% of the women investigated, two of whom were found to be IgM positive, while no difference could be determined between pregnant and non-pregnant women. Antigenemia assay was negative in all cases. HCMV gB gene amplification was found in 5.1 and 8.5% of WBCs and in 10 and 14.5% of vaginal secretion from pregnant and non-pregnant women, respectively. CONCLUSION: The high seroprevalence observed is in accordance with previous Brazilian surveys. Antigenemia assay was unable to detect the occurrence of active infection in the immunocompetent women studied, most likely because it either occurred in a viral load undetectable by this assay or did not occur at all. Although the highest incidence of positivity was observed by gene amplification both in WBCs and secretion from non-pregnant than in pregnant women, the rate of viral detection was statistically similar for both groups.

Cytomegalovirus in human abortion in Espírito Santo, Brazil.

BACKGROUND: Despite the established implication of human cytomegalovirus (HCMV) in congenital infection, there are still conflicting reports regarding the association of HCMV with spontaneous abortion. Viral antigens and nucleic acid were already described in tissues from abortions cases, but did not indicate HCMV pathogenical role. OBJECTIVES: (1) To access viral seroprevalence (total and IgM antibodies) in pregnant, non-pregnant and in women in abortion process, (2) to evaluate if antigenemia assay can detect active infection in these populations, (3) to detect viral DNA in peripheral leukocytes, and (4) in abortion tissues. STUDY DESIGN: Blood samples from 95 patients in abortion process and from two control groups (40 pregnant and 60 non-pregnant women) were obtained for determination of viral seroprevalence, for detection of antigen and viral DNA by PCR from peripheral leukocytes. Specimens obtained from 88 patients in abortion process, spontaneous or induced, were submitted to gB gene amplification (PCR and nested-PCR). RESULTS: Viral seroprevalence were found in 97.3 with 2.5% of IgM positive cases. Antigenemia assay were negative in all cases, however, viral nucleic acid were found in 6.3 and in 6.0% of the patients in abortion and in control groups, respectively. Nucleic acid in conception tissue was present in 6.6%. CONCLUSION: This high seroprevalence observed is according to previous surveys in Brazil. If active infection due to viral reactivation occurred during the abortion process, it cannot be accessed by antigenemia or anti-IgM assays. Nucleic acid found by PCR in peripheral blood cells enriched with polymorphonuclear cells (PMN) corresponds to viral circulation in immunocompetent person, as similar results were found for the three groups. Although viral DNA had been found in 6.6% from abortion tissues, this result does not support HCMV as a major abortion-related factor as we could not found any correlation between abortion and active HCMV infection.