1.
Biochem Biophys Res Commun
; 251(1): 235-8, 1998 Oct 09.
Article
in English
| MEDLINE
| ID: mdl-9790937
ABSTRACT
We have developed a baculovirus expression system for the rapid and efficient production of large quantities (>10 mg/l) of VP16. The recombinant VP16 binds to a complex of host cell transcription factors and TAATGARAT motif. Secondary structure calculations from circular dichroism measurements indicate a content of 32.0 % alpha-helix and 17.5 % beta-sheet. This is the first structural CD analysis of VP16 which will be very useful for high-throughput assay development and mechanistic studies.
Subject(s)
Herpes Simplex Virus Protein Vmw65/biosynthesis , Herpes Simplex Virus Protein Vmw65/chemistry , Herpesvirus 1, Human/chemistry , Baculoviridae/genetics , Circular Dichroism , Gene Expression Regulation, Viral , Herpes Simplex Virus Protein Vmw65/genetics , Protein Structure, Secondary , Recombinant Proteins/biosynthesis , Recombinant Proteins/isolation & purification
2.
J Pract Nurs
; 30(11): 40-2, 55, 1980.
Article
in English
| MEDLINE
| ID: mdl-6904496