Engineering of highly selective variants of Parvibaculum lavamentivorans alcohol dehydrogenase.

We present the development of highly selective variants of the Parvibaculum lavamentivorans alcohol dehydrogenase. Four amino acids (A158, N162, K202, L224) in the second sphere of the catalytic site were identified to determine the selectivity for 3-quinuclidone reduction significantly. The best variant (A158H/N162G/K202Q/L224W) was able to increase the ee for (R)-3-quinuclidinol production from 84.3 % (wild-type) to ≥99 % and concomitantly to enhance conversion by 43.5 %.