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1.
Arq. bras. med. vet. zootec ; 65(4): 981-988, Aug. 2013. graf, tab
Article in Portuguese | LILACS | ID: lil-684451

ABSTRACT

Foram colhidas amostras de 100 carcaças em um frigorífico exportador, localizado no interior do estado de São Paulo, amostradas ao longo de um ano, por meio do método de esponjas, aplicado na região do peito do animal. As amostras foram colhidas em três pontos, denominados A, B e C, sendo cada carcaça amostrada nos três pontos, localizados nas etapas: pós-sangria (A); pós-esfola (B) e pós-lavagem (C). Foram realizadas pesquisas de Listeria sp., E. coli O157, Salmonella spp. e microrganismos indicadores (Petrifilms® AC, EC e EB). Não foram isolados Listeria ou E. coli O157 em nenhuma das 300 amostras. Salmonella spp. foi isolada em nove, sendo oito no ponto A e uma no ponto B. Para mesófilos, as contagens variaram de 0 a 6,8 log UFC/cm², para coliformes totais, de 0 a 4,57 log UFC/cm², e para E. coli, de 0 a 4,38 log UFC/cm². Diante dos resultados obtidos e em comparação com a literatura, conclui-se que o estabelecimento estudado apresenta qualidade, tanto sanitária (devido às baixas prevalências dos patógenos) quanto higiênica (devido à acentuada diminuição da carga microbiana de indicadores ao longo da linha).


Samples were collected from 100 carcasses in a slaughterhouse exporter, located within the State of São Paulo, sampled over a year through the sponge method, applied to the chest of the animal. Samples were taken at three points, denominated A, B and C, each carcass sampled at three points located in the following steps: after bleeding (A) after skinning (B) and after washing (C). Research was conducted for Listeria sp., E. coli O157, Salmonella spp. and Micro-organism (Petrifilms ® AC, EC and EB). Listeria or E. coli O157 were not isolated in any of the 300 samples. Salmonella spp. was isolated in nine, eight at point A and one at point B. For Mesophiles, scores ranged from 0 to 6.8 log UFC/cm²; for Total coliforms, 0 to 4.57 log UFC/cm² and E. coli from 0 to 4.38 log UFC/cm². With the results obtained and compared with the literature, it is concluded that the establishment in this study has both sanitary quality (due to the low prevalence of pathogens) and hygienic quality (due to the sharp decrease in the microbial load of indicators along the line.


Subject(s)
Animals , Cattle , Food Preservatives , Meat/analysis , Listeria monocytogenes/pathogenicity , Salmonella/pathogenicity , Cattle/classification
2.
J Food Prot ; 75(5): 973-5, 2012 May.
Article in English | MEDLINE | ID: mdl-22564950

ABSTRACT

Since 2004, Brazil has been the leading exporter of chicken. Because of the importance of this sector in the Brazilian economy, food safety must be ensured by control and monitoring of the production stages susceptible to contamination, such as the chilling process. The goal of this study was to evaluate changes in microbial levels on chicken carcasses and in chilling water after immersion in a chilling system for 8 and 16 h during commercial processing. An objective of the study was to encourage discussion regarding the Brazilian Ministry of Agriculture Livestock and Food Supply regulation that requires chicken processors to completely empty, clean, and disinfect each tank of the chilling system after every 8-h shift. Before and after immersion chilling, carcasses were collected and analyzed for mesophilic bacteria, Enterobacteriaceae, coliforms, and Escherichia coli. Samples of water from the chilling system were also analyzed for residual free chlorine. The results do not support required emptying of the chiller tank after 8 h; these tanks could be emptied after 16 h. The results for all carcasses tested at the 8- and 16-h time points indicated no significant differences in the microbiological indicators evaluated. These data provide both technical and scientific support for discussing changes in federal law regarding the management of immersion chilling water systems used as part of the poultry processing line.


Subject(s)
Chickens/microbiology , Food Contamination/analysis , Food Handling/methods , Food-Processing Industry/methods , Animals , Brazil , Chlorine/analysis , Chlorine/pharmacology , Cold Temperature , Enterobacteriaceae/isolation & purification , Escherichia coli/isolation & purification , Food Contamination/prevention & control , Food-Processing Industry/standards , Humans , Time Factors
3.
Dermatol Surg ; 37(10): 1486-98, 2011 Oct.
Article in English | MEDLINE | ID: mdl-21883649

ABSTRACT

BACKGROUND: Endovenous laser ablation (EVLA) was performed in the treatment of great and small saphenous veins (GSVs, SSVs), perforating veins (PVs), and varicose collaterals (VCs). OBJECTIVE To verify the outcome in PVs and VCs. MATERIALS AND METHODS: Four hundred eighty-two limbs of 306 patients were studied. EVLA was performed on 167 GSVs, 52 SSVs, and 534 PVs of 303 limbs and on VCs of 467 limbs; 133 GSVs were stripped, 300 of saphenofemoral junctions (SFJs) and 45 saphenopopliteal junctions (SPJs) were interrupted. Limbs were selected using duplex ultrasound examination and photographs; PVs-VCs diameter (<4 mm) and VC length were measured. EVLA was performed using a 808-nm diode laser, 0.6-mm fibers, continuous emission, 4 to 10 W, and 10 to 20 J/cm. Follow-up on 467 limbs occurred over a mean 27.5 months (range 3 months to 6 years); 98 limbs were followed up for longer than 4 years. RESULTS: Operating time range from 10 to 30 minutes per limb. Blood vaporization, thrombosis, fibrosis, and atrophy prevailed in PVs and in the large VCs (>4 mm) and massive coagulation in the smaller (<4 mm). High rate of occlusion was seen, with different rates of patent PV-VC mainly in diameter >6 mm. Thirty-nine out of 511 patent PVs (7.6%) and 96 out of 778 VCs (12-13%) were re-treated using EVLA or foam sclerotherapy. Minor complications occurred in 88 of the 778 (11%). CONCLUSIONS: EVLA of PVs and VCs is effective and faster than surgery in 2- to 6-mm PVs and VCs using an 808-nm diode laser.


Subject(s)
Lasers, Semiconductor/therapeutic use , Low-Level Light Therapy , Varicose Veins/radiotherapy , Venous Insufficiency/radiotherapy , Adolescent , Adult , Aged , Aged, 80 and over , Female , Humans , Leg/blood supply , Male , Middle Aged , Ultrasonography, Doppler, Duplex , Varicose Veins/diagnostic imaging , Venous Insufficiency/diagnostic imaging , Young Adult
4.
Minerva Cardioangiol ; 51(4): 395-404, 2003 Aug.
Article in English | MEDLINE | ID: mdl-12900721

ABSTRACT

Various surgical techniques have been proposed for the treatment of chronic venous insufficiency of post-thrombotic recanalized deep veins of the lower limbs. The preferable method seems to be represented by intravenous valvuloplasty except for the cases affected by extensive valvular damage. For this reason some experimental autologous, heterologous and prosthetic venous valves have been proposed. Such a problem emerged for 1 patient (male, aged 78 years, right limb, leg dystrophy, multiple ulcerations at the ankle) which was selected by duplex, Doppler venous pressure index, photoplethysmography and ascending phlebography. An iliac-femoral and popliteal post-thrombotic, recanalized, decompensated venous insufficiency and one Cockett's perforator incompetence were diagnosed (CEAP classification: C6s Es As2d14 Pr). A bicuspid apparently repairable popliteal valve was detected by phlebography. A traditional intravenous valvuloplasty was planned but the valve was not found at surgical exploration. A monocuspid valve reconstruction by intimal flap vein was performed. The following results were obtained and controlled after one year: stable ulceration healing, dystrophy reduction, improvement in the quality of life, normalization of the hemodynamic parameters and of the radiological morphology of the new valve. It can be concluded that monocuspid valvular repair by intimal flap can be successfully performed in cases affected by secondary valveless deep venous insufficiency of the lower limbs.


Subject(s)
Popliteal Vein/surgery , Surgical Flaps , Venous Insufficiency/surgery , Aged , Humans , Leg Ulcer/etiology , Male , Plethysmography , Popliteal Vein/diagnostic imaging , Popliteal Vein/pathology , Ultrasonography , Venous Insufficiency/diagnosis
5.
Minerva Anestesiol ; 68(7-8): 631-4, 2002.
Article in Italian | MEDLINE | ID: mdl-12244296

ABSTRACT

Oncologic diseases frequently need a central venous catheterization to improve pharmacological administration safety and patient's comfort. We report a case of a woman affected by acute myelocytic leukemia with a bilateral stenosis of the innominate veins, likely of thrombotic nature, diagnosed during central venous catheterization. These events, as that occurred to our patient, are usually caused by hypercoagulability inducted by oncologic diseases, sepsis, antithrombin III deficiency, catheters materials and repeated catheterizations. Although the treatment, based on local thrombolysis, systemic heparinization, and surgery to repair venous obstruction, is effective, the prevention of such events is fundamental. It can be achieved with catheters of particular characteristics and appropriate management techniques. Finally it is underlined that in oncology patients, before catheterization, especially when the objective examination is negative, radiological methodologies and in particular ultrasonography are an important aid to establish the presence or absence of thrombosis in internal jugular, subclavian and innominate veins.


Subject(s)
Brachiocephalic Veins , Leukemia, Myeloid, Acute/complications , Vascular Diseases/physiopathology , Adult , Catheterization, Central Venous , Humans , Male , Thrombolytic Therapy , Vascular Diseases/etiology , Vascular Diseases/therapy
6.
Minerva Anestesiol ; 68(11): 861-5, 2002 Nov.
Article in English | MEDLINE | ID: mdl-12538969

ABSTRACT

The stiff-person syndrome is a rare and disabling disorder, characterized by muscle rigidity with superimposed painful spasms involving axial and limb musculature. The clinical symptoms are continuous contraction of agonist and antagonist muscles caused by involuntary motor-unit firing at rest and the spasms that are precipitated by tactile stimuli, passive strach, volitional movement of affected or unaffected muscles, startling noises and emotional stimuli. Both the rigidity and the spasms are relieved by sleep, general anaesthesia, myoneural blockade and peripheral nerve blockade. The cause of the stiff-person syndrome is unknown but an autoimmune pathogenesis is suspected because 1) the presence in the cerebrospinal fluid (CSF) of antibodies against glutamic acid decarboxylase (GAD), the rate-limiting enzyme for the synthesis of the inhibitory neurotrasmitter gamma-aminobutyric acid (GABA), 2) the association of the disease with other autoimmune disorders, 3) the presence of various autoantibodies and 4) a strong immunogenetic association. The stiff-person syndrome is clinically elusive but potentially treatable and should be considered in patients with unexplained stiffness and spasms. Drugs that enhance GABA neurotransmission, such as diazepam, vigabatrin and baclofen, provide modest relief of clinical symptoms. Immunomodulatory agents such as steroids, plasmapheresis and intravenous immunoglobulin, seem to offer substantial improvement.


Subject(s)
Stiff-Person Syndrome/therapy , Aged , Aged, 80 and over , Diazepam/therapeutic use , Female , Glutamate Decarboxylase/cerebrospinal fluid , Glutamate Decarboxylase/metabolism , Humans , Muscle Relaxants, Central/therapeutic use , Stiff-Person Syndrome/diagnosis , Stiff-Person Syndrome/physiopathology
7.
Minerva Anestesiol ; 60(1-2): 71-5, 1994.
Article in Italian | MEDLINE | ID: mdl-8208456

ABSTRACT

The author reports an unusual case of voluntary poisoning caused by 60 mg of colchicine. He describes the physiopathological mechanism of poisoning, the clinical evolution and the treatment. In particular, he examines the ARDS, typical complication, analyzing the responsible mechanism of this event and some of its peculiarities. The author pays particular attention to the blood monitoring levels of HHb as a precious diagnostic index of diffuse infiltrative lung disease.


Subject(s)
Colchicine/poisoning , Suicide, Attempted , Adult , Humans , Male
8.
Minerva Anestesiol ; 58(12): 1339-42, 1992 Dec.
Article in Italian | MEDLINE | ID: mdl-1294922

ABSTRACT

The Authors present a case of a young man who drowned in sea water after the shock caused by a lightening that fell close to him as the swam. The Authors discuss and analyze the effects of the two events (drowning and lightening) which developed similar cerebral injuries but with a different origin.


Subject(s)
Brain Injuries/etiology , Lightning Injuries/complications , Near Drowning/complications , Adolescent , Humans , Male
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