ABSTRACT
In experiments in vivo the effect of nanocerium (cerium oxide nanoparticles) on the stability of red blood cells to acid hemolysis, levels of both ROS and RNS generation and H2S pools in plasma and erythrocytes of old rats were investigated. In red blood cells of old rats the proton penetration into the matrix of erythrocytes showed a significant raising and the fate of labile "aging" erythrocytes in old animals compared with adult were up- regulated. These phenomena paralleled with significant up-regulation of ROS and RNS generation. Introduction for 14 days per os to old rats 0.1 mg/kg of nanocerium fully restored resistance of erythrocytes to acid hemolysis by ROS and RNS in both plasma and erythrocytes reduction. Nanocerium decreased the erythrocytes and, conversely, significantly increased the plasma's pools of H2S.
Subject(s)
Aging/blood , Cerium/administration & dosage , Erythrocytes/drug effects , Nanoparticles/administration & dosage , Reactive Nitrogen Species/antagonists & inhibitors , Reactive Oxygen Species/antagonists & inhibitors , Administration, Oral , Aging/pathology , Animals , Animals, Outbred Strains , Cells, Cultured , Cerium/metabolism , Erythrocytes/cytology , Erythrocytes/metabolism , Hemolysis/drug effects , Hydrogen Sulfide/blood , Nanoparticles/metabolism , Osmotic Fragility/drug effects , Oxidative Stress/drug effects , Rats , Reactive Nitrogen Species/blood , Reactive Oxygen Species/bloodABSTRACT
The results of research on changes in the level of apoptosis among the spleen cells during the development of intravaginal staphylococcal infection in experimental models of different genetic mice lines are given in the paper. Intravaginal injection of Staphylococcus aureus resulted in an increased number of cells with morphological characteristics of apoptosis in underbred and line BALB/c mice, while it decreased in the ICR line mice. The level of the apoptotic cells in the underbred and line BALB/c mice was accompanied by some level of microorganisms that were seeded out of kidneys--the higher was the number of microorganisms, the more apoptotic cells were indicated.