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1.
J Biomech Eng ; 143(3)2021 03 01.
Article in English | MEDLINE | ID: mdl-33269788

ABSTRACT

Myocardial bridging (MB) and coronary atherosclerotic stenosis can impair coronary blood flow and may cause myocardial ischemia or even heart attack. It remains unclear how MB and stenosis are similar or different regarding their impacts on coronary hemodynamics. The purpose of this study was to compare the hemodynamic effects of coronary stenosis and MB using experimental and computational fluid dynamics (CFD) approaches. For CFD modeling, three MB patients with different levels of lumen obstruction, mild, moderate, and severe were selected. Patient-specific left anterior descending (LAD) coronary artery models were reconstructed from biplane angiograms. For each MB patient, the virtually healthy and stenotic models were also simulated for comparison. In addition, an in vitro flow-loop was developed, and the pressure drop was measured for comparison. The CFD simulations results demonstrated that the difference between MB and stenosis increased with increasing MB/stenosis severity and flowrate. Experimental results showed that increasing the MB length (by 140%) only had significant impact on the pressure drop in the severe MB (39% increase at the exercise), but increasing the stenosis length dramatically increased the pressure drop in both moderate and severe stenoses at all flow rates (31% and 93% increase at the exercise, respectively). Both CFD and experimental results confirmed that the MB had a higher maximum and a lower mean pressure drop in comparison with the stenosis, regardless of the degree of lumen obstruction. A better understanding of MB and atherosclerotic stenosis may improve the therapeutic strategies in coronary disease patients and prevent acute coronary syndromes.


Subject(s)
Myocardial Bridging
2.
Radiat Res ; 186(2): 175-88, 2016 08.
Article in English | MEDLINE | ID: mdl-27387860

ABSTRACT

Hemodynamic shear stress is defined as the physical force exerted by the continuous flow of blood in the vascular system. Endothelial cells, which line the inner layer of blood vessels, sense this physiological force through mechanotransduction signaling and adapt to maintain structural and functional homeostasis. Hemodynamic flow, shear stress and mechanotransduction signaling are, therefore, an integral part of endothelial pathophysiology. Although this is a well-established concept in the cardiovascular field, it is largely dismissed in studies aimed at understanding radiation injury to the endothelium and subsequent cardiovascular complications. We and others have reported on the differential response of the endothelium when the cells are under hemodynamic flow shear compared with static culture. Further, we have demonstrated significant differences in the gene expression of static versus shear-stressed irradiated cells in four key pathways, reinforcing the importance of shear stress in understanding radiation injury of the endothelium. This article further emphasizes the influence of hemodynamic shear stress and the associated mechanotransduction signaling on physiological functioning of the vascular endothelium and underscores its significance in understanding radiation injury to the vasculature and associated cardiac complications. Studies of radiation effect on endothelial biology and its implication on cardiotoxicity and vascular complications thus far have failed to highlight the significance of these factors. Factoring in these integral parts of the endothelium will enhance our understanding of the contribution of the endothelium to radiation biology. Without such information, the current approaches to studying radiation-induced injury to the endothelium and its consequences in health and disease are limited.


Subject(s)
Endothelium, Vascular/cytology , Endothelium, Vascular/radiation effects , Hemodynamics/radiation effects , Humans , Mechanotransduction, Cellular/radiation effects , Models, Biological , Stress, Mechanical
3.
Circ Cardiovasc Interv ; 5(4): 499-507, 2012 Aug 01.
Article in English | MEDLINE | ID: mdl-22763346

ABSTRACT

BACKGROUND: Stent luminal surface characteristics influence surface endothelialization. We hypothesize that luminal stent microgrooves created in the direction of coronary flow accelerate endothelial cell migration, resulting in lower levels of neointimal formation. METHODS AND RESULTS: Surface coverage efficiency was evaluated in vitro by allowing human aortic endothelial cells (HAEC) to migrate onto microgrooved (G) or smooth (NG) surfaces. HAEC functionality was assessed by proliferation rate, apoptosis rate, nitric oxide production, and inflammatory markers TNF-α and VCAM-1 expression. Early endothelialization and restenosis studies were performed using the porcine coronary injury model. Stainless steel stents of identical design with (GS) and without (NGS) luminal microgrooves were used. The commercially available Multi-Link Vision (MLVS) stent of identical design was used as a control. The degree of GS and NGS surface endothelialization was compared at 3 days. Biocompatibility and tissue response outcomes were evaluated at 28 days. The in vitro study demonstrated that at 7 days the presence of surface microgrooves increased HAEC migration distance >2-fold. Cell proliferation rate and nitric oxide production were increased and apoptosis rate was decreased. There was no difference in inflammatory marker expression. At 3 days, coronary artery stent endothelialization was significantly increased in GS compared with NGS (81.3% versus 67.5%, P=0.0002). At 28 days, GS exhibited lower neointimal thickness compared with either NGS (21.1%, P=0.011) or MLVS (40.8%, P=0.014). CONCLUSION: Parallel microgrooves on coronary stent luminal surfaces promote endothelial cell migration and positively influence endothelial cell function, resulting in decreased neointimal formation in the porcine coronary injury model.


Subject(s)
Coronary Vessels/cytology , Coronary Vessels/injuries , Endothelial Cells/cytology , Prosthesis Design/methods , Stents , Wound Healing/physiology , Animals , Aorta/cytology , Apoptosis , Cell Movement/physiology , Cell Proliferation , Cells, Cultured , Chromium Alloys , Coronary Artery Disease/pathology , Coronary Artery Disease/therapy , Coronary Circulation/physiology , Coronary Restenosis/pathology , Coronary Restenosis/prevention & control , Disease Models, Animal , Endothelial Cells/physiology , Humans , Neointima/pathology , Neointima/prevention & control , Stainless Steel , Sus scrofa
4.
Cell Mol Bioeng ; 3(3): 286-295, 2010 Sep 01.
Article in English | MEDLINE | ID: mdl-21116478

ABSTRACT

Intimal hyperplasia (IH) remains the major cause of intermediate and long-term failure of vascular grafts and endovascular interventions. Arteries are subjected to a significant longitudinal stress in addition to the shear stress and tensile stress from the blood flow. The aim of this study was to determine the effect of axial stretch on cell proliferation and IH in arteries. Porcine carotid arteries, intact or endothelial cell (EC) denudated, were maintained ex vivo at different stretch ratios (1.3, 1.5, and 1.8) and flow rates (16 or 160 mL/min) while remaining at physiologic pressure for 7 days. The viability of the arteries was verified with norepinephrine, carbachol, and sodium nitroprusside stimulations, and the cell proliferation was detected using bromodeoxyuridine labeling and immunostaining. Our results showed that the axial stretch ratio did not significantly affect intimal thickness and cell proliferation in normal arteries. However, axial stretch increased the neointimal thickness in EC denudated arteries cultured under low flow conditions. The cell proliferation increased significantly in the intima and inner half of the media of the EC denudated arteries under normal or elevated axial stretch in comparison to intact arteries at the same stretch ratio. These results demonstrated that axial stretch with EC denudation and low flow increases neointimal formation and cell proliferation in the arteries.

5.
Wound Repair Regen ; 18(2): 159-67, 2010.
Article in English | MEDLINE | ID: mdl-20230600

ABSTRACT

We describe the fabrication and use of an in vitro wounding device that denudes cultured epithelium in patterns designed to leave behind strips or islands of cells sufficiently narrow or small to ensure that all the remaining cells become rapidly activated and then migrate, dedifferentiate, and proliferate in near synchrony. The design ensures that signals specific to regenerating cells do not become diluted by quiescent differentiated cells that are not affected by wound-induced activation. The device consists of a flat circular disk of rubber, engraved to produce alternating ridges and grooves in patterns of concentric circles or parallel lines. The disk is mounted at the end of a pneumatically controlled piston assembly. Application of controlled pressure and circular or linear movement of the disk on cultures produced highly reproducible wounding patterns. The near-synchronous regenerative activity of cell bands or islands allowed the collection of samples large enough for biochemical studies to sensitively detect alterations involving mRNA for several early response genes and protein phosphorylation in major signaling pathways. The method is versatile, easy to use and reproducible, and should facilitate biochemical, proteomic, and genomic studies of wound-induced regeneration of cultured epithelium.


Subject(s)
Epithelium/physiology , Regeneration , Wound Healing/physiology , Animals , Blotting, Western , Cell Movement , Cell Proliferation , Cells, Cultured , Genes, fos , Genes, jun , Microscopy , Polymerase Chain Reaction , Proto-Oncogene Proteins c-jun/genetics , RNA, Messenger/metabolism
6.
J Mater Sci Mater Med ; 20(10): 2105-16, 2009 Oct.
Article in English | MEDLINE | ID: mdl-19466532

ABSTRACT

The role of metal microstructure (e.g. grain sizes) in modulating cell adherence behavior is not well understood. This study investigates the effect of varying grain sizes of 316L stainless steel (SS) on the attachment and spreading of human aortic endothelial cells (HAECs). Four different grain size samples; from 16 to 66 microm (ASTM 9.0-4.9) were sectioned from sheets. Grain structure was revealed by polishing and etching with glycergia. Contact angle measurement was done to assess the hydrophilicity of the specimens. Atomic force microscopy (AFM) and X-ray photoelectron spectroscopy (XPS) were used to characterize the roughness and surface chemistry of the specimens. Cells were seeded on mechanically polished and chemically etched specimens followed by identification of activated focal adhesion sites using fluorescently tagged anti-pFAK (phosphorylated focal adhesion kinase). The 16 microm grain size etched specimens had significantly (P < 0.01) higher number of cells attached per cm(2) than other specimens, which may be attributed to the greater grain boundary area and associated higher surface free energy. This study shows that the underlying material microstructure may influence the HAEC behavior and may have important implications in endothelialization.


Subject(s)
Endothelial Cells/drug effects , Endothelial Cells/physiology , Metal Nanoparticles , Stainless Steel/chemistry , Stainless Steel/pharmacology , Adhesiveness , Aorta/cytology , Cell Adhesion , Cell Count , Cell Proliferation/drug effects , Cells, Cultured , Endothelial Cells/cytology , Focal Adhesions/drug effects , Focal Adhesions/physiology , Humans , Materials Testing , Metal Nanoparticles/chemistry , Microscopy, Atomic Force , Particle Size , Photoelectron Spectroscopy
8.
Curr Opin Investig Drugs ; 8(3): 219-25, 2007 Mar.
Article in English | MEDLINE | ID: mdl-17408117

ABSTRACT

Percutaneous intervention using balloon angioplasty accompanied by stent implantation has become the predominant procedure to treat occlusive coronary and peripheral vascular disease. Unfortunately, restenosis associated with intimal hyperplasia and arterial remodeling at the stented site occurs within 6 months in 20 to 30% of cases. To address this problem, the concept of utilizing a stent as the vehicle to deliver agents locally and limit the overexuberant tissue response related to its placement has been developed. Targeting excess arterial wall smooth muscle cell proliferation, preclinical studies have demonstrated the efficacy of two drugs, paclitaxel and rapamycin, in both in vitro and in vivo animal studies. Early, as well as large, randomized clinical studies using polymer-coated, drug-eluting stents have clearly demonstrated a significant and dramatic efficacy in reducing restenosis rates and improving clinical outcomes compared with the use of the bare stent for revascularization procedures. Despite the low incidence of late thrombosis associated with the rapamycin- and paclitaxel-eluting stents, some concerns remain (such as the need for sustained anticoagulant therapy), providing the impetus for developing coated stents that promote rather than inhibit endothelial healing in order to limit the restenotic response.


Subject(s)
Cardiovascular Agents/therapeutic use , Cardiovascular Diseases/drug therapy , Drug Design , Stents , Animals , Cardiovascular Agents/pharmacology , Cardiovascular Diseases/complications , Cardiovascular Diseases/metabolism , Coronary Restenosis/complications , Coronary Restenosis/prevention & control , Drug Evaluation, Preclinical , Humans , Models, Biological , Randomized Controlled Trials as Topic
9.
Am J Physiol Cell Physiol ; 290(5): C1321-33, 2006 May.
Article in English | MEDLINE | ID: mdl-16601147

ABSTRACT

In a variety of disorders, overaccumulation of lipid in nonadipose tissues, including the heart, skeletal muscle, kidney, and liver, is associated with deterioration of normal organ function, and is accompanied by excessive plasma and cellular levels of free fatty acids (FA). Increased concentrations of FA may lead to defects in mitochondrial function found in diverse diseases. One of the most important regulators of mitochondrial function is mitochondrial Ca(2+) ([Ca(2+)](m)), which fluctuates in coordination with intracellular Ca(2+) ([Ca(2+)](i)). Polyunsaturated FA (PUFA) have been shown to cause [Ca(2+)](i) mobilization albeit by unknown mechanisms. We have found that PUFA but not monounsaturated or saturated FA cause [Ca(2+)](i) mobilization in NT2 human teratocarcinoma cells. Unlike the [Ca(2+)](i) response to the muscarinic G protein-coupled receptor agonist carbachol, PUFA-mediated [Ca(2+)](i) mobilization in NT2 cells is independent of phospholipase C and inositol-1,4,5-trisphospate (IP(3)) receptor activation, as well as IP(3)-sensitive internal Ca(2+) stores. Furthermore, PUFA-mediated [Ca(2+)](i) mobilization is inhibited by the mitochondria uncoupler carboxyl cyanide m-chlorophenylhydrozone. Direct measurements of [Ca(2+)](m) with X-rhod-1 and (45)Ca(2+) indicate that PUFA induce Ca(2+) efflux from mitochondria. Further studies show that ruthenium red, an inhibitor of the mitochondrial Ca(2+) uniporter, blocks PUFA-induced Ca(2+) efflux from mitochondria, whereas inhibitors of the mitochondrial permeability transition pore cyclosporin A and bongkrekic acid have no effect. Thus PUFA-gated Ca(2+) release from mitochondria, possibly via the Ca(2+) uniporter, appears to be the underlying mechanism for PUFA-induced [Ca(2+)](i) mobilization in NT2 cells.


Subject(s)
Calcium Signaling/drug effects , Calcium/metabolism , Fatty Acids, Unsaturated/administration & dosage , Mitochondria/metabolism , Teratocarcinoma/metabolism , Cell Line, Tumor , Dose-Response Relationship, Drug , Humans , Intracellular Fluid/metabolism , Mitochondria/drug effects
10.
J Endovasc Ther ; 12(5): 594-604, 2005 Oct.
Article in English | MEDLINE | ID: mdl-16212461

ABSTRACT

PURPOSE: To establish a reproducible laboratory test to evaluate prospective vascular biomaterials with respect to their thromboinflammatory properties by examining fibrinogen, platelet, and monocyte binding. Endothelial migration onto these surfaces was used as an index of vascular healing. METHODS: To evaluate biomaterials for potential thrombogenicity and inflammation, binding assays of radiolabeled human fibrinogen, platelets, and monocytes were performed on standard pieces of vascular biomaterials, including metals and polymeric and ceramic-coated materials. Using an established in vitro endothelial cell migration model, the relative migration rate of cultured human aortic endothelial cells onto these vascular biomaterials was measured and compared. The fibrinogen, platelet, and monocyte binding results were combined along with the migration results to create an overall score of biocompatibility. RESULTS: A significant direct relation of platelet and monocyte binding to the amount of adsorbed fibrinogen was observed. In contrast, migration rates of cultured human aortic endothelial cells onto the same biomaterial surfaces were found to be inversely related the amount of bound fibrinogen. Among the materials tested, stainless steel received the highest score of biocompatibility, while turbostratic carbon scored the lowest. CONCLUSIONS: Fibrinogen, platelet, and monocyte binding levels, as well as endothelial migration rates onto vascular material surfaces, provide a basis for evaluating thrombogenicity, inflammatory potential, and endothelialization in the laboratory prior to in vivo testing.


Subject(s)
Biocompatible Materials/pharmacology , Endothelium, Vascular/drug effects , Alloys/metabolism , Alloys/pharmacology , Aorta/cytology , Aorta/drug effects , Binding, Competitive/drug effects , Biocompatible Materials/metabolism , Blood Platelets/metabolism , Cell Adhesion/drug effects , Cell Movement/drug effects , Chromium Alloys/metabolism , Chromium Alloys/pharmacology , Cobalt/metabolism , Cobalt/pharmacology , Endothelial Cells/drug effects , Endothelium, Vascular/cytology , Fibrinogen/metabolism , Humans , Materials Testing/methods , Materials Testing/standards , Monocytes/metabolism , Polyhydroxyethyl Methacrylate/metabolism , Polyhydroxyethyl Methacrylate/pharmacology , Polytetrafluoroethylene/metabolism , Polytetrafluoroethylene/pharmacology , Polyurethanes/metabolism , Polyurethanes/pharmacology , Reproducibility of Results , Thrombosis/metabolism , Thrombosis/physiopathology
11.
Am J Physiol Cell Physiol ; 284(4): C1039-47, 2003 Apr.
Article in English | MEDLINE | ID: mdl-12620896

ABSTRACT

We have investigated the role of inhibitor kappaBalpha (IkappaBalpha) in the activation of nuclear factor kappaB (NF-kappaB) observed in human aortic endothelial cells (HAEC) undergoing a low shear stress of 2 dynes/cm(2). Low shear for 6 h resulted in a reduction of IkappaBalpha levels, an activation of NF-kappaB, and an increase in kappaB-dependent vascular cell adhesion molecule 1 (VCAM-1) mRNA expression and endothelial-monocyte adhesion. Overexpression of IkappaBalpha in HAEC attenuated all of these shear-induced responses. These results suggest that downregulation of IkappaBalpha is the major factor in the low shear-induced activation of NF-kappaB in HAEC. We then investigated the role of nitric oxide (NO) in the regulation of IkappaBalpha/NF-kappaB. Overexpression of endothelial nitric oxide synthase (eNOS) inhibited NF-kappaB activation in HAEC exposed to 6 h of low shear stress. Addition of the structurally unrelated NO donors S-nitrosoglutathione (300 microM) or sodium nitroprusside (1 mM) before low shear stress significantly increased cytoplasmic IkappaBalpha and concomitantly reduced NF-kappaB binding activity and kappaB-dependent VCAM-1 promoter activity. Together, these data suggest that NO may play a major role in the regulation of IkappaBalpha levels in HAEC and that the application of low shear flow increases NF-kappaB activity by attenuating NO generation and thus IkappaBalpha levels.


Subject(s)
Endothelium, Vascular/physiology , Glutathione/analogs & derivatives , I-kappa B Proteins/physiology , NF-kappa B/metabolism , Nitric Oxide/physiology , Aorta/cytology , Aorta/physiology , Cells, Cultured , Down-Regulation , Endothelium, Vascular/cytology , Glutathione/pharmacology , Humans , Nitric Oxide/antagonists & inhibitors , Nitric Oxide Donors/pharmacology , Nitric Oxide Synthase/physiology , Nitric Oxide Synthase Type III , Nitro Compounds/pharmacology , Nitroprusside/pharmacology , Promoter Regions, Genetic/drug effects , RNA, Messenger/metabolism , Stress, Mechanical , Vascular Cell Adhesion Molecule-1/genetics
12.
Oncogene ; 21(22): 3541-51, 2002 May 16.
Article in English | MEDLINE | ID: mdl-12032856

ABSTRACT

TGFbeta overexpression in human cancer cells has been shown to promote tumor progression. In the present study, we sought to determine whether sequestration of endogenous TGFbeta by the expression of a soluble TGFbeta type III receptor (sRIII), can reduce malignancy in human carcinoma cells and whether the tumor-suppressive activity of sRIII is associated with the inhibition of angiogenesis. Ectopic expression of sRIII significantly inhibited the growth of tumors formed by human colon carcinoma HCT116 and breast carcinoma MDA-MB-435 cells in nude mice. It also reduced the metastatic potential of the MDA-MB-435 cells. Thus, endogenous TGFbeta appears to be necessary for the progression of these two carcinomas. Furthermore, when the tumor cells were mixed with Matrigel and embedded subcutaneously in nude mice, the blood volume in Matrigel plugs containing sRIII-expressing cells as indicated by hemoglobin levels was significantly lower than that in Matrigel plugs containing the respective control cells. Blood vessel counts in paraffin sections of the Matrigel plugs containing sRIII-expressing cells were also significantly lower than those in paraffin sections of the Matrigel plugs containing control cells. Treatment of human endothelial cells with a recombinant sRIII significantly inhibited their ability to form a capillary web structure on Matrigel. These results for the first time indicate that the sRIII-induced tumor suppression appears to be in part due to the inhibition of angiogenesis.


Subject(s)
Angiogenesis Inhibitors/genetics , Carcinoma/therapy , Neovascularization, Pathologic/therapy , Proteoglycans/chemistry , Proteoglycans/genetics , Receptors, Transforming Growth Factor beta/chemistry , Receptors, Transforming Growth Factor beta/genetics , Angiogenesis Inhibitors/chemistry , Angiogenesis Inhibitors/metabolism , Angiogenesis Inhibitors/pharmacology , Animals , Antineoplastic Agents/chemistry , Antineoplastic Agents/metabolism , Antineoplastic Agents/pharmacology , Breast Neoplasms/blood supply , Breast Neoplasms/pathology , Breast Neoplasms/therapy , Carcinoma/blood supply , Carcinoma/pathology , Cell Division , Cell Line , Cells, Cultured , Colonic Neoplasms/blood supply , Colonic Neoplasms/pathology , Colonic Neoplasms/therapy , Endothelium, Vascular/cytology , Endothelium, Vascular/drug effects , Female , Humans , Kinetics , Mice , Mice, Nude , Neoplasm Metastasis , Protein Structure, Tertiary , Proteoglycans/metabolism , Receptors, Transforming Growth Factor beta/metabolism , Transfection , Tumor Cells, Cultured
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