ABSTRACT
Polycythemia vera (PV) is a chronic myeloproliferative disease. The use of recombinant alpha 2a Interferon (IFN) therapy in this disease is a novel approach. We applied Fourier-transform infrared microspectroscopy (FT-IR-M) to investigate the behavior and therapeutic responsiveness of PV patients treated with IFN. A spectroscopic parameter (A1/A2) was used, corresponding to the ratio of the integrated areas of the bands at 1080 cm-1 and at 1540 cm-1 due to nucleic acids and proteic components, respectively, calculated on the spectra of single megakaryocytes (MKs). In previous studies, we have pointed out that MKs in PV have a surprisingly strong myeloproliferative impulse when compared to MKs from other chronic myeloproliferative diseases. Nine patients out of the 11 studied exhibited a satisfactory responsiveness to the IFN treatment. Ten patients were evaluated by the A1/A2 parameter. In 8 of these, a good agreement was seen between this parameter and the laboratory data commonly used for the assessment of this disease. The infrared parameter, which we propose, proves to be an original, reliable method for the evaluation of recombinant alpha 2a IFN responsiveness in this disease.
Subject(s)
Interferon-alpha/therapeutic use , Megakaryocytes/chemistry , Polycythemia Vera/blood , Polycythemia Vera/therapy , Blood Cell Count , Hematocrit , Hemoglobins/analysis , Humans , Interferon alpha-2 , L-Lactate Dehydrogenase/blood , Recombinant Proteins , Spectroscopy, Fourier Transform Infrared/methodsSubject(s)
Adenocarcinoma , Leukemia, Myelogenous, Chronic, BCR-ABL Positive , Leukemia, Myelogenous, Chronic, BCR-ABL Positive/complications , Neoplasms, Multiple Primary , Prostatic Neoplasms , Adenocarcinoma/drug therapy , Aged , Androgen Antagonists/therapeutic use , Busulfan/therapeutic use , Cyproterone/analogs & derivatives , Cyproterone/therapeutic use , Cyproterone Acetate , Humans , Leukemia, Myelogenous, Chronic, BCR-ABL Positive/drug therapy , Male , Prostatic Neoplasms/drug therapy , Remission InductionSubject(s)
Fourier Analysis , Leukemia, Lymphoid/blood , Spectrophotometry, Infrared , Child , Child, Preschool , HumansSubject(s)
Leukemia, Myeloid/genetics , Adult , Humans , Karyotyping , Leukemia, Myeloid/diagnosis , Male , Middle Aged , Philadelphia ChromosomeSubject(s)
Primary Myelofibrosis/pathology , Aged , Fixatives , Flow Cytometry , Humans , Male , Peroxidases/analysis , Primary Myelofibrosis/enzymologyABSTRACT
Ten samples of lymphocytes coming from patients affected by chronic lymphatic leukemia and ten samples from normal subjects were studied by FT-IR spectroscopy. Spectral differences observed between the two kinds of cells correspond to an increase of the intensities, in the leukemic samples with respect to the normal ones, of the bands corresponding mainly to PO2- symmetrical and asymmetrical stretching vibrations of DNA. The ratios of the integrated areas of the band at 1080 cm-1 mainly involving the symmetrical stretching vibration of the O-P-O linkages of DNA, and of the band at 1540 cm-1, due to the proteic components of the lymphocytes, assume different values for the two kinds of cells. These ratios can constitute an additional marker to diagnose chronic lymphatic leukemia and may be usefully employed to evidence the early phases of the disease.
Subject(s)
Leukemia/blood , Lymphocytes/pathology , Chronic Disease , DNA, Neoplasm/analysis , Fourier Analysis , Humans , Leukemia/genetics , RNA, Neoplasm/analysis , Spectrophotometry, InfraredABSTRACT
Two autopsy cases of angioimmunoblastic lymphadenopathy with dysproteinemia were examined. Clinical and morphological data did not differ greatly from those in other cases of the literature: patients died two and three months after the onset of the symptoms. The autopsy findings included moderately enlarged generalized lymph nodes, hepatosplenomegaly and lungs involvement. Microscopically the most prominent change was the vascular proliferation with pleiomorphic cellular infiltration.
Subject(s)
Blood Protein Disorders/pathology , Immunoblastic Lymphadenopathy/pathology , Aged , Hepatomegaly/pathology , Humans , Immunoblastic Lymphadenopathy/blood , Liver/pathology , Lymph Nodes/pathology , Male , Spleen/pathology , Splenomegaly/pathologyABSTRACT
Two series of normal and leukaemic lymphocytes were examined by infrared spectroscopy in order to try to find spectral differences connected with chemical and biological modifications. The bands at 965 and 530 cm-1 present only in the spectra of leukaemic lymphocytes, assume particular significance. The C-H stretching region furnishes useful indications about the different ratios of the methyl groups compared with the methylene ones in the two cases. The infrared bands characteristic of the leukaemic lymphocytes seem to be due to chemical modifications not involving the DNA chain.
Subject(s)
Leukemia, Lymphoid/pathology , Lymphocytes/cytology , Humans , Reference Values , Spectrophotometry, Infrared/methodsABSTRACT
Eighteen months after the occurrence of Waldenström's macroglobulinemia, Philadelphia (Ph1) chromosome-positive chronic myeloid leukemia developed in a 69-year-old woman. The coexistence of the two disorders was characterized by an initial reduction of paraproteinemia at the time that leukemia occurred, a long-lasting remission of the two disorders, and a final parallel increase of paraproteins and WBCs. Since leukemia occurred 15 months after the interruption of melphalan therapy, the potential mutagenic role of chemotherapy was considered irrelevant. Therefore, Waldenström's macroglobulinemia might favor the occurrence of chronic myeloid leukemia.
