Phytochemical Analysis and Antioxidant Evaluation of the Ethanolic Extract of the Leaves of Abutilon indicum.

BACKGROUND: In Indian traditional medicine, the Abutilon indicum plant, colloquially known as "Country mallow" or "Thuthi", has been vouched for its efficacy in treating conditions such as bronchitis and diabetes. The study aimed to explore the chemical constituents and antioxidant strength of the ethanolic extracts derived from the leaves of this plant (ELEAI). OBJECTIVES: To qualitatively pinpoint the phytochemicals in the ethanolic extract of abutilon indicum leaves (ELEAI), utilize high-performance thin layer chromatography (HPTLC) to quantitatively analyze the identified compounds within the ELEAI, and gauge its antioxidant capability through the DPPH method, benchmarking the outcomes against the recognized standard, ascorbic acid. METHODS: Abutilon indicum leaves, originating from Telangana, were authenticated by taxonomists at Osmania University. After cleaning and drying, the leaves were powdered. A mixture of ethanol and water (70:30 ratio) was then used to extract the compounds in a Soxhlet extractor for a duration of 72 hours at a temperature of 60°C. The liquid extract was subsequently evaporated to form a light-brown powder, which was stored at 20°C under shade for later use. RESULTS: Preliminary analyses indicated that ELEAI was rich in both primary and secondary metabolites. Luteolin, a known phytochemical, was quantitatively confirmed in the extract using HPTLC. Impressively, the DPPH assay highlighted ELEAI's remarkable antioxidant capabilities. CONCLUSION: Abutilon indicum showcases notable therapeutic potential with its rich phytochemical content and strong antioxidant activity, making it a promising candidate for further pharmacological research and natural drug formulation.

A Comprehensive Analysis of Phytochemical Composition, Acute Toxicity Assessment, and Antioxidant Potential of Ethanolic Extract of Carica Papaya Seeds.

BACKGROUND: Carica papaya seeds are rich in phytochemicals with potential health benefits, warranting safety and antioxidant assessments. This study comprehensively examined the ethanolic extract of Carica papaya seeds (EECPS) to elucidate its phytochemical composition, acute toxicity profile, and antioxidant activity. METHODS: Phytochemical analysis of EECPS revealed the presence of various bioactive compounds, including flavonoids, tannins, phenols, alkaloids, proteins, glycosides, and saponins. Additionally, the presence of sulfuric acid was confirmed. Acute toxicity assessment involved oral administration of EECPS at 2000mg/kg body weight to Wistar rats, with a 14-day observation period. General parameters, body weight changes, and histopathological examination of kidney and liver tissues were evaluated. Antioxidant activity was assessed using the 2,2-Diphenyl-1-picrylhydrazyl (DPPH) assay, and the half-maximal inhibitory concentration (IC50) value of EECPS was compared to that of gallic acid. RESULTS: Phytochemical analysis confirmed the diverse composition of EECPS, suggesting its potential health benefits and biological activity. Acute toxicity assessment revealed no adverse effects, with rats exhibiting normal behavior, weight stability, and no histopathological abnormalities in vital organs. The gallic acid IC50 value was determined to be 5.73±0.02 µg/mL, indicating its antioxidant potency. EECPS exhibited antioxidant properties in a dose-dependent manner, with higher concentrations demonstrating increased DPPH free radical quenching capacity. The IC50 value for EECPS was calculated from the dose-response curve to be 39.41±1.61 µg/mL (expressed as mean ± standard error of the mean (SEM)). CONCLUSION: The phytochemical analysis of EECPS highlights its diverse composition and potential health benefits. Acute toxicity studies in rats confirm its safety for oral administration, with no adverse effects observed. EECPS exhibits significant antioxidant activity, as indicated by its IC50 value. These findings suggest that EECPS holds promise for therapeutic use and health applications. However, further research is needed to determine its precise antioxidant potential. Subchronic and chronic toxicity studies are recommended to establish its safety profile definitively and unlock its full potential for healthcare and nutrition.

Evaluation of the Ethanolic Leaf Extract of Abutilon indicum on Isonicotinic Acid Hydrazide-Induced Proinflammatory Marker Gene Expression Changes.

BACKGROUND: Abutilon indicum, widely found in India, Sri Lanka, and parts of America and Malaysia, is renowned for its rich bioactive compounds including alkaloids, flavonoids, and sesquiterpene lactones. Due to its diverse pharmacological potential, it has garnered significant attention in traditional medicine. In particular, the ethanolic leaf extract of Abutilon indicum (ELEAI) has demonstrated anti-inflammatory effects, notably targeting the 5-lipoxygenase enzyme pivotal in inflammatory responses. OBJECTIVE: This study aimed to elucidate the impact of the ELEAI on proinflammatory marker gene expression induced by isoniazid (INH). METHODS: A total of 36 rats were systematically divided into six experimental groups. The control group received DMSO orally for the initial 30 days followed by distilled water for the subsequent 30 days. The INH group received a daily dose of INH (30 mg/kg b.w., i.p.) for 30 days and the rats were then sacrificed on day 30. The ELEAI (250 mg/kg) group was administered INH daily for 30 days, followed by daily post-treatment with ELEAI (250 mg/kg) for another 30 days. Similarly, the ELEAI (500 mg/kg) group received INH daily for 30 days, followed by daily post-treatment with ELEAI (500 mg/kg) for another 30 days. The silymarin (SIL) group was given INH daily for 30 days, followed by post-treatment with SIL at a dose of 100 mg/kg body weight daily for the subsequent 30 days. Finally, the ELEAI (500 mg/kg) alone group was administered distilled water orally for the first 30 days and then received ELEAI at a dose of 500 mg/kg b.w. orally once daily for the next 30 days. RESULTS: Continuous INH exposure for a month led to a pronounced increase in proinflammatory genes like TNF-α, TGF-ß, and NF-kB and a decrease in the IkB gene in rat liver tissues. Subsequent treatment with SIL (100 mg/kg) and ELEAI (250 and 500 mg/kg) post-INH exposure resulted in a marked decrease in proinflammatory genes and a surge in IkB expression. CONCLUSION: The findings suggest that the ELEAI exerts a dose-responsive influence on proinflammatory activities. Notably, A. indicum counteracts inflammation, especially that triggered by bradykinin and prostaglandins. The ELEAI showcases promising therapeutic potential, exhibiting both pro and anti-inflammatory properties and antiproliferative characteristics.

Membrane Assisted Palatal Fistula Closure in a Cleft Palate Patient: A Novel Technique.

Palatal fistula following cleft palate repair, is one of the considerable complications and remains a challenging problem to the surgeons. The reported recurrence rate of the fistula is between 33% to 37%. Due to fibrosis and poor vascularity of adjacent tissues, high recurrence rates are typical. Closure of palatal fistulas can be achieved by different surgical techniques like local, regional and distant flaps, local turnover flaps, pedicled flaps from oral mucosa, buccal fat pad flaps, inter-positional cartilage grafts can be utilized for management of small fistulas. For larger fistualas, tongue flaps, temporalis muscle flaps, musculomucosal flaps, nasal septal flaps and free flaps can be used. These procedures are often cumbersome and leave a raw nasal or oral surface, which may increase the incidence of postoperative problems or some flaps can be bulky and may require a second-stage procedure. Different synthetic materials such as alloderm, Poly-D and L-Lactic Acid or "PdLLA" and collagen membrane are used in multilayer repair represented by the nasal mucosa, the inter-positional graft and oral mucosa. These interpositional grafts provide a scaffold for in growth of tissues, revascularization and mucosal epithelialization. We present a case of closure of an oronasal fistula, using resorbable collagen membrane in three layered repair to avoid recurrence.

Intralesional Sclerotherapy - A Novel Approach for The Treatment of Intraoral Haemangiomas.

In infancy and childhood haemangiomas are the most common neoplasms in head and neck region with an occurrence of about 60%. Approximately 40%-50% of all haemangiomas resolve incompletely, leaving permanent changes in the skin, such as telangiectases, epidermal atrophy, hypopigmentation or redundant skin with fibro-fatty residue but a few stubborn, problematic haemangiomas may result in serious disfigurement and dysfunction, and even become life-threatening. Most haemangiomas are managed by conservative methods that include corticosteroids (either systemic or local injection), sclerotherapy, interferon-alpha, laser therapy, embolization, cryotherapy, and radiation. Due to risk of haemorrhaging, surgical removal for small vascular lesions is not considered and it is more invasive than sclerotherapy. This case report presents the efficacy of intralesional sclerotherapy for the management of haemangiomas on dorsum and lateral border of tongue.