Sputum versus bronchoscopy for diagnosis of Pseudomonas aeruginosa biofilms in cystic fibrosis.

The present authors hypothesised that bronchoscopy with protected specimen brush may sample biofilm-forming bacteria adherent to the airway wall, whereas traditional sputum collection may not. Pseudomonas aeruginosa obtained from sputum, bronchoalveolar lavage and protected brush, taken from the right upper lung bronchus of 12 adult patients with cystic fibrosis, were compared. Retrieved bacteria were genotyped, and grown in planktonic cultures and as biofilms, and susceptibilities to individual antibiotics and to antibiotic combinations were determined. Bacterial cultures obtained using bronchoscopy did not yield any new strains of bacteria that were not also found in sputum. A total of 10 patients (83%) had a single strain of P. aeruginosa found using sputum, bronchoalveolar lavage and protected brush techniques, and two patients (17%) had two strains recovered in sputum, but only one strain was recovered using bronchoscopic techniques. Susceptibility to single antibiotics and to antibiotic combinations were not different between planktonically or biofilm-grown bacteria derived from sputum, as compared to those obtained by bronchoalveolar lavage and protected brush. In conclusion, sputum collection provides as much information as bronchoscopy for characterising the genotype and antibiotic susceptibility of chronic Pseudomonas aeruginosa infection in patients with stable cystic fibrosis.

Socio-environmental factors and postnatal depressive symptomatology: a longitudinal study.

This study analyses the relationships between stressful life conditions and postnatal depressive symptomatology in a group of women of low socioeconomic status (SES) and a group of women of high SES from the third to the ninth week postpartum. Nulliparous pregnant women were recruited from the prenatal care clinics of four hospitals. Multiple linear regression analyses demonstrated that after accounting for SES group membership and depressive symptomatology during pregnancy, early postnatal chronic stressors (frequent conflictual episodes with network members, maternal health problems) and social support were linked to later postnatal depressive symptomatology.

Depressive symptoms in the late postpartum among low socioeconomic status women.

BACKGROUND: Postpartum depression has been the focus of much research in the past 15 years, but little is known about factors associated with depression of longer duration or later onset. The purpose of this longitudinal study was to analyze the relationship between stressful life conditions and postnatal depression in a group of women of low socioeconomic status from the third week to the sixth month postpartum. METHODS: Nulliparas who met criteria for low socioeconomic status were recruited from the prenatal care clinics of four Montreal hospitals. Questionnaires were verbally administered in the home at 30 weeks' gestation, at 3 and 9 weeks postpartum, and at 6 months postpartum. Blockwise multiple linear regression analyses were performed by entering predictor variables that included sociodemographic characteristics, chronic stressors, life events, and social support network. RESULTS: Sixty-eight women participated in the study. At 6 months postpartum, 38.2 percent of the mothers had a Beck Depression Inventory score of 10 or more. After accounting for previous depression, analyses indicated that chronic stressors (maternal health problems, infant difficulty, lack of money for basic needs, frequent conflicts with network members) and poor social support (informational and emotional) were associated with postnatal depressive symptoms. CONCLUSIONS: Health practitioners should recognize that high depressive symptomatology frequently occurs among low socioeconomic status first-time mothers at six months postpartum. Chronic stressors and inadequate social support are the most important factors associated with this problem.

Contingent tolerance, compensatory responses, and physical dependence in diazepam-treated amygdala-kindled rats.

Three groups of amygdala-kindled rats received 10 bidaily treatment trials: On each trial, the drug-before group received a diazepam (2.5 mg/kg i.p.) injection 1 hr before a convulsive stimulation, the drug-after group received a diazepam injection 1 hr after a stimulation, and the vehicle control group received a vehicle injection either 1 hr before or 1 hr after a stimulation. After treatment, only the drug-before group displayed significantly longer forelimb clonus under the influence of diazepam (that is, they displayed contingent tolerance to diazepam's anticonvulsant effect) and significantly longer forelimb clonus while drug free. Following a 14-day retention period, the rats in the drug-before group retained significant levels of contingent tolerance but did not display significant increases when tested drug free. These data suggest that compensatory responses do not play a causal role in the expression of contingent tolerance.

[Social support network evolution after the birth of their first baby: comparison between lower and middle class mothers]. / Evolution du reseau de soutien social lors d'une premiere naissance : comparaison entre des meres defavorisees et des meres de classe moyenne.

The social support networks of a group of low socio-economic status (S.E.S.) mothers (n = 87) and a group of higher S.E.S. mothers (n = 44) are described and compared at the 30th week of pregnancy and at the third week after the birth of their first baby. The evolution of the support networks for these two groups of mothers during this period is also examined. A modified form of the ASSIS from Barrera has been used to measure the social support network. Results show that, during pregnancy, the social support network of low-S.E.S. mothers is more restricted than that of higher S.E.S. mothers. Although the actual number of people around them after the birth of the baby did not increase, low-S.E.S. mothers said they felt a slight increase in the number of people available to give support in some way. They also reported that conflicts were more frequent with some of them. For higher S.E.S. mothers, all social network variables remained stable from pregnancy through the first postpartum month.

Chronic stressors, social support, and depression during pregnancy.

OBJECTIVES: To analyze the relationships between stressful life conditions, social support, and depressive symptomatology during pregnancy in women of low socioeconomic status and a comparison group of women of higher socioeconomic status. METHODS: Study participants were recruited from four hospital prenatal care clinics. Low socioeconomic status was defined as no more than 11 years of education and a household income below the poverty level. Higher socioeconomic status was defined as at least 12 years of education and a household income at least one and one-half times the poverty level. All subjects were nulliparous, over 18 years of age, and French-speaking. Questionnaires were administered verbally at the participants' homes during the 30th week of pregnancy, approximately. The Beck Depression Inventory was used to measure depressive symptomatology during the preceding 7 days. RESULTS: Approximately 47% of the low socioeconomic status women and 20% of the higher socioeconomic status women scored 10 or more on the Beck Depression Inventory, indicating a depressive state. Multiple regression analysis demonstrated that chronic stressors (eg, financial and housing problems), negative life events, and inadequate social support were all linked to high depressive symptomatology during pregnancy. CONCLUSION: During pregnancy, depressive symptoms are common, especially in women of low socioeconomic status, and are strongly related to socioenvironmental factors.