ABSTRACT
The staphylococcal bi-component leukotoxins constitute a family included in the super-family of the beta-sheet-structured pore-forming toxins. They may be produced by Staphylococcus aureus and by Staphylococcus intermedius and their target cells vary according to the molecules. The mode of action proceeds by the sequential binding of the class S proteins, then by that of the class F proteins at the surface of the membranes. Then, the activation of cellular calcium-channels precedes the pore formation which seems to be sensitive to several monovalent cations. The cell response is inflammatory and includes the neosynthesis as well as the secretion of leukotriene B4, interleukin -8, histamine. The injection of leukotoxins to rabbits generates cell chemotaxis , vasodilatation, and tissue necrosis. The association of the production of leukotoxins with clinical syndromes concerns several aspects of the pathology of S. aureus, and confers to these leukotoxins an important role of virulence factors.
Subject(s)
Bacterial Proteins/pharmacology , Bacterial Toxins/pharmacology , Cell Membrane Permeability/drug effects , Erythrocytes/drug effects , Hemolysin Proteins , Leukocidins/pharmacology , Neutrophils/drug effects , Staphylococcus aureus/metabolism , T-Lymphocytes/drug effects , Animals , Bacterial Proteins/metabolism , Bacterial Toxins/metabolism , Calcium Channels/metabolism , Cations, Divalent/metabolism , Cattle , Chemotaxis, Leukocyte/drug effects , Cross Infection/pathology , Cross Infection/physiopathology , Exotoxins , Female , Histamine Release/drug effects , Humans , Interleukin-8/metabolism , Ion Transport , Leukocidins/metabolism , Leukotriene B4/metabolism , Male , Mastitis, Bovine/physiopathology , Models, Biological , Necrosis , Rabbits , Staphylococcal Infections/pathology , Staphylococcal Infections/physiopathology , Staphylococcal Infections/veterinary , Vasodilation/drug effects , Virulence , Vitreous BodyABSTRACT
The ability of leukotoxins secreted by Staphylococcus aureus to modify the permeability of the membrane of human polymorphonuclear neutrophils has been studied by spectrofluorometry and appropriate fluorescent probes. This family of bicomponent leukotoxins is constituted by, at least, three pairs of proteins: LukS-PV/LukF-PV, HlgA/HlgB, HlgC/HlgB. After binding of both components to the membrane, each pair induces influxes of divalent cations and ethidium in polymorphonuclear neutrophils, although with different intensities. The influx of divalent cations appears sooner than the influx of ethidium. The pathway for divalent cations is not permeable to monovalent cations (Na+, K+, ethidium+) and is blocked by Ca2+ channel inhibitors that do not block the fluxes of ethidium and monovalent cations. It is concluded that the leukotoxins bind to a receptor linked to a divalent cation-selective channel or to the channel itself which is activated. Then, the leukotoxins open a second pathway by insertion into the membrane and subsequent formation of aspecific pores allowing an influx of ethidium.