Photopatterning enzymes on polymer monoliths in microfluidic devices for steady-state kinetic analysis and spatially separated multi-enzyme reactions.

A method for photopatterning multiple enzymes on porous polymer monoliths within microfluidic devices has been developed and used to perform spatially separated multienzymatic reactions. To reduce nonspecific adsorption of enzymes on the monolith, its pore surface was modified by grafting poly(ethylene glycol), followed by surface photoactivation and enzyme immobilization in the presence of a nonionic surfactant. Characterization of bound horseradish peroxidase (HRP) was carried out using a reaction in which the steady-state profiles of the fluorescent reaction product could be measured in situ and then analyzed using a plug-flow bioreactor model to determine the observed maximum reaction rate and Michaelis constant. The Michaelis constant of 1.9 micro mol/L agrees with previously published values. Mass-transfer limitations were evident at relatively low flow rates but were absent at higher flow rates. Sequential multienzymatic reactions were demonstrated using the patternwise assembly of two- and three-enzyme systems. Glucose oxidase (GOX) and HRP were patterned in separate regions of a single channel, and product formation was analyzed as a function of flow direction. Significant product formation occurred only in the GOX to HRP direction. A three-enzyme sequential reaction was performed using invertase, GOX, and HRP. All possible arrangements of the three enzymes were tested, but significant product formation was only observed when the enzymes were in the correct sequential order. Photopatterning enzymes on polymer monoliths provides a simple technique for preparing spatially localized multiple-enzyme microreactors capable of directional synthesis.

Hydrophilic surface modification of cyclic olefin copolymer microfluidic chips using sequential photografting.

The plastic material known as cyclic olefin copolymer (COC) is a useful substrate material for fabricating microfluidic devices due to its low cost, ease of fabrication, excellent optical properties, and resistance to many solvents. However, the hydrophobicity of native COC limits its use in bioanalytical applications. To increase surface hydrophilicity and reduce protein adsorption, COC surfaces were photografted with poly(ethylene glycol) methacrylate (PEGMA) using a two-step sequential approach: covalently-bound surface initiators were formed in the first step and graft polymerization of PEGMA was then carried out from these sites in the second step. Contact angle measurements were used to monitor and quantify the changes in surface hydrophilicity as a function of grafting conditions. As water droplet contact angles decreased from 88 degrees for native COC to 45 degrees for PEGMA-grafted surfaces, protein adsorption was also reduced by 78% for the PEGMA-modified COC microchannels as determined by a fluorescence assay. This photografting technique should enable the use of COC microdevices in a variety of bioanalytical applications that require minimal nonspecific adsorption of biomolecules.

Chip electrochromatography.

Electrochromatography (EC) in microfluidic chips is emerging as an attractive alternative to capillary electrophoresis (CE) for on-chip separations. This review summarizes recent developments in the rapidly growing area of chip electrochromatography with a focus on "column" technologies. Relevant achievements are summarized according to the types of stationary phase used for the separations including open channels, microfabricated structures, and channels packed with beads or containing a porous monolith. The advantages and disadvantages of each, as well as practical aspects of their application, are discussed. The analytical performance of these devices is demonstrated with separations involving various families of compounds mostly in the reversed-phase chromatographic mode.

Fabrication of porous polymer monoliths covalently attached to the walls of channels in plastic microdevices.

UV-initiated grafting of plastic tubes and microfluidic chips with ethylene diacrylate followed by the preparation of porous polymer monoliths has been studied. The first step affords a thin grafted layer of polymer with a multiplicity of pendent double bonds that are then used in the second step for covalent attachment of the monolith to the wall. As clearly seen on scanning electron micrographs, this procedure prevents the formation of voids at the monolith-channel interface a problem that has always plagued approaches involving bulk polymerization in nontreated channels due to the shrinkage of the monolith during the polymerization process and its lack of compatibility with the material of the device. Irradiation with UV light through a photomask allows precise patterning specifying both the area subjected to surface modification and the location of the monolith within specific areas of the device.