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Bone Marrow Transplant ; 25 Suppl 2: S83-7, 2000 May.
Article in English | MEDLINE | ID: mdl-10933197

ABSTRACT

An allogeneic tumor cell vaccine should display a natural immunogenicity that allows the stimulation of tumor-reactive effector cells in patients. Furthermore, the vaccine should express antigens that are shared by many tumors to which patients are not tolerant. A variety of tumor peptides should be presented by different HLA-molecules due to limited MHC matching with recipients and last but not least, the vaccine should have a strong growth potential in vitro to allow adequate amounts of vaccine to be generated for long-term usage. In vitro and in situ studies with the renal cell carcinoma cell line RCC-26 demonstrate: (1) RCC-26 can induce complex allospecific responses through direct priming; (2) RCC-26 can not only reactivate cytotoxic T lymphocytes (CTL) of a memory phenotype but they also can induce de novo tumor-antigen associated responses in normal donors; (3) these cells present epitopes restricted by several MHC molecules, allowing the vaccination of patients matched for different HLA alleles; and (4) they stimulate HLA-A*0201-restricted T cells bearing characteristic T cell receptors (TCR). Thus, in addition to using limiting dilution killer and ELISPOT assays, molecular tracking of a tumor-specific TCR can be used to judge the development of antitumor reactivity and vaccine efficiency.


Subject(s)
Cancer Vaccines/therapeutic use , Carcinoma, Renal Cell/therapy , Kidney Neoplasms/therapy , Carcinoma, Renal Cell/genetics , Carcinoma, Renal Cell/immunology , Cytotoxicity Tests, Immunologic , Humans , In Vitro Techniques , Kidney Neoplasms/genetics , Kidney Neoplasms/immunology , Metals, Rare Earth , Monitoring, Immunologic , Neoplasm Transplantation , Receptors, Antigen, T-Cell/genetics , Reverse Transcriptase Polymerase Chain Reaction , T-Lymphocytes, Cytotoxic/immunology , Transplantation, Homologous , Tumor Cells, Cultured , Vaccination
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