Subject(s)
Cats/metabolism , Copper/analysis , Hair/chemistry , Zinc/analysis , Animals , Castration/veterinary , Cats/blood , Copper/blood , Female , Male , Reference Values , Zinc/bloodABSTRACT
Low capacity, high affinity [3H] dexamethasone binding receptors were identified in cytosolic preparations of liver (mean number 45 +/- 10.1 fmol mg-1 protein, apparent dissociation constant 0.4 +/- 0.1 nM) and skin (mean number 46.4 +/- 23.8 fmol mg-1 protein, apparent dissociation constant 1 +/- 0.2 nM) of clinically normal dogs. For clinically normal cats, approximately half these numbers of receptors with a lower affinity, were detected in liver (mean number 23.1 +/- 10.4 fmol mg-1 protein, apparent dissociation constant 3.2 +/- 0.9 nM) and skin (mean number 23.90 +/- 10.9 fmol mg-1 protein, apparent dissociation constant 2.2 +/- 1.5 nM). This difference between dogs and cats in [3H] dexamethasone binding receptors may contribute to the relative glucocorticoid resistance observed in cats.
Subject(s)
Cats/metabolism , Dogs/metabolism , Epidermis/metabolism , Liver/metabolism , Receptors, Glucocorticoid/analysis , Animals , Dexamethasone/metabolism , Epidermis/chemistry , Liver/chemistry , RatsABSTRACT
Low capacity, high affinity [3H] dexamethasone binding receptors were identified in cytosolic preparations of the skin (mean number 42.0 +/- 25.2 fmol mg-1 protein, apparent dissociation constant (1 nM +/- 0.23) of clinically normal dogs. No [3H] dexamethasone binding was observed in the skin of nine out of 10 dogs with confirmed spontaneous hyperadrenocorticism or in the skin of three out of six dogs with undiagnosed hormonal alopecia. A reduction was detected in the number of [3H] dexamethasone binding receptors in the skin of one dog with confirmed hypothyroidism. This study provides evidence for the susceptibility of canine glucocorticoid receptors to down regulation by imbalances of endogenous hormones, particularly increased glucocorticoid concentrations.
Subject(s)
Dexamethasone/metabolism , Dog Diseases/metabolism , Endocrine System Diseases/veterinary , Receptors, Glucocorticoid/analysis , Skin/chemistry , Adrenocortical Hyperfunction/metabolism , Adrenocortical Hyperfunction/veterinary , Alopecia/metabolism , Alopecia/veterinary , Animals , Dogs , Endocrine System Diseases/metabolism , Hypothyroidism/metabolism , Hypothyroidism/veterinaryABSTRACT
The concentrations of zinc in serum, leucocytes and hair of normal dogs, dogs with zinc-responsive dermatosis and dogs with dermatitis not associated with zinc deficiency was determined. The mean concentration of zinc in serum and hair of dogs with zinc-responsive dermatosis was significantly lower than in other dogs but the range of zinc concentrations overlapped that of the other dogs. The mean leucocyte zinc concentration was similar for each group of dogs. It is concluded that low zinc concentrations in serum and hair have only a corroborative value in the diagnosis of zinc-responsive dermatosis in dogs.
Subject(s)
Dog Diseases/etiology , Hair/analysis , Leukocytes/analysis , Skin Diseases/veterinary , Zinc/analysis , Animals , Dog Diseases/blood , Dogs , Skin Diseases/blood , Skin Diseases/etiology , Zinc/bloodABSTRACT
The triacylglycerols and cholesterol in the sera of a group of Nigerian diabetics were found to be higher than in a corresponding control group, but did not exceed the range normal to Caucasians. The total cholesterol and HDL-cholesterol in the normal Nigerians were both lower than the levels reported for Caucasians although the value for the ratio total cholesterol: HDL-cholesterol did not differ. A low level of HDL-cholesterol in the blood is not in itself an indicator of atheroma. If the total cholesterol is also low, as occurs in the African, the distribution of the cholesterol between the lipoprotein classes is not disturbed.
