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1.
BMC Genomics ; 13: 50, 2012 Jan 31.
Article in English | MEDLINE | ID: mdl-22292898

ABSTRACT

BACKGROUND: The liver X receptors (LXRs) are oxysterol sensing nuclear receptors with multiple effects on metabolism and immune cells. However, the complete genome-wide cistrome of LXR in cells of human origin has not yet been provided. RESULTS: We performed ChIP-seq in phorbol myristate acetate-differentiated THP-1 cells (macrophage-type) after stimulation with the potent synthetic LXR ligand T0901317 (T09). Microarray gene expression analysis was performed in the same cellular model. We identified 1357 genome-wide LXR locations (FDR < 1%), of which 526 were observed after T09 treatment. De novo analysis of LXR binding sequences identified a DR4-type element as the major motif. On mRNA level T09 up-regulated 1258 genes and repressed 455 genes. Our results show that LXR actions are focused on 112 genomic regions that contain up to 11 T09 target genes per region under the control of highly stringent LXR binding sites with individual constellations for each region. We could confirm that LXR controls lipid metabolism and transport and observed a strong association with apoptosis-related functions. CONCLUSIONS: This first report on genome-wide binding of LXR in a human cell line provides new insights into the transcriptional network of LXR and its target genes with their link to physiological processes, such as apoptosis.The gene expression microarray and sequence data have been submitted collectively to the NCBI Gene Expression Omnibus http://www.ncbi.nlm.nih.gov/geo under accession number GSE28319.


Subject(s)
Chromatin/metabolism , Gene Expression Regulation , Genome-Wide Association Study , Macrophages/metabolism , Orphan Nuclear Receptors/metabolism , Apoptosis , Binding Sites , Cell Line , Down-Regulation , Humans , Lipid Metabolism , Liver X Receptors , Oligonucleotide Array Sequence Analysis , Orphan Nuclear Receptors/antagonists & inhibitors , Protein Binding , Up-Regulation
2.
Biochim Biophys Acta ; 1788(5): 1176-87, 2009 May.
Article in English | MEDLINE | ID: mdl-19306841

ABSTRACT

The cervical epithelial cell line, HeLa, is one of the oldest and most commonly used cell lines in cell biology laboratories. Although a truncated P2X(7) receptor has recently been identified in HeLa cells, the expression of other purinergic receptors or the function of the P2X(7) protein has not been characterized. We here show that HeLa cells express transcripts for most P2X and P2Y purinergic receptors. Treatment of cells with ATP or other P2X(7) agonists does not stimulate cell death, but can induce atypical calcium fluxes and ion currents. Cervical epithelial cells represent an important target for sexually-transmitted pathogens and are commonly exposed to pro-inflammatory cytokines such as IFNgamma. Stimulation of HeLa cells with IFNgamma upregulates expression of P2X(7) mRNA and full-length protein, modifies ATP-dependent calcium fluxes, and renders the cells sensitive to ATP-induced apoptosis, which can be blocked by a P2X(7) antagonist. IFNgamma treatment also increased dramatically the sensitivity of the intestinal epithelial cell line, HCT8, to ATP-induced apoptosis. Significantly, IFNgamma also stimulated P2X(7) expression on human intestinal tissues. Responses to other purinergic receptor ligands suggest that HeLa cells may also express functional P2Y(1), P2Y(2) and P2Y(6) receptors, which could be relevant for modulating ion homeostasis in the cells.


Subject(s)
Interferon-gamma/pharmacology , Receptors, Purinergic P2/metabolism , Receptors, Purinergic/metabolism , Adenosine Triphosphate/pharmacology , Base Sequence , Calcium Signaling/drug effects , Cell Line , DNA Primers/genetics , Epithelial Cells/drug effects , Epithelial Cells/metabolism , HeLa Cells , Humans , Ion Transport/drug effects , Purinergic P2 Receptor Agonists , RNA, Messenger/genetics , RNA, Messenger/metabolism , Receptors, Purinergic/genetics , Receptors, Purinergic P2/genetics , Receptors, Purinergic P2X7 , Recombinant Proteins , Up-Regulation/drug effects , Uridine Triphosphate/pharmacology
3.
J Immunol ; 179(6): 3707-14, 2007 Sep 15.
Article in English | MEDLINE | ID: mdl-17785807

ABSTRACT

Ligation of the purinergic receptor, P2X7R, with its agonist ATP has been previously shown to inhibit intracellular infection by chlamydiae and mycobacteria in macrophages. The effect of P2X7R on chlamydial infection had never been investigated in the preferred target cells of chlamydiae, cervical epithelial cells, nor in vaginally infected mice. In this study, we show that treatment of epithelial cells with P2X7R agonists inhibits partially Chlamydia infection in epithelial cells. Chelation of ATP with magnesium or pretreatment with a P2X7R antagonist blocks the inhibitory effects of ATP. Similarly to previous results obtained with macrophages, ATP-mediated inhibition of infection in epithelial cells requires activation of host-cell phospholipase D. Vaginal infection was also more efficient in P2X7R-deficient mice, which also displayed a higher level of acute inflammation in the endocervix, oviduct, and mesosalpingeal tissues than in infected wild-type mice. However, secretion of IL-1beta, which requires P2X7R ligation during infection by other pathogens, was decreased mildly and only at short times of infection. Taken together, these results suggest that P2X7R affects Chlamydia infection by directly inhibiting infection in epithelial cells, rather than through the ability of P2X7R to modulate IL-1beta secretion.


Subject(s)
Cervix Uteri/metabolism , Chlamydia Infections/pathology , Chlamydia Infections/prevention & control , Genital Diseases, Female/prevention & control , Receptors, Purinergic P2/physiology , Adenosine Triphosphate/metabolism , Adenosine Triphosphate/physiology , Animals , Cell Line, Tumor , Cervix Uteri/immunology , Cervix Uteri/microbiology , Chlamydia Infections/genetics , Chlamydia Infections/immunology , Chlamydia muridarum/growth & development , Chlamydia muridarum/immunology , Chronic Disease , Epithelial Cells/immunology , Epithelial Cells/metabolism , Epithelial Cells/microbiology , Fallopian Tubes/metabolism , Fallopian Tubes/pathology , Female , Genital Diseases, Female/immunology , Genital Diseases, Female/metabolism , Genital Diseases, Female/microbiology , Genital Diseases, Female/pathology , HeLa Cells , Humans , Interleukin-1beta/antagonists & inhibitors , Interleukin-1beta/metabolism , Mice , Mice, Inbred C57BL , Mice, Inbred DBA , Mice, Knockout , Purinergic P2 Receptor Agonists , RNA, Messenger/biosynthesis , Receptors, Purinergic P2/deficiency , Receptors, Purinergic P2/metabolism , Receptors, Purinergic P2X7
4.
Cell Microbiol ; 8(6): 1047-57, 2006 Jun.
Article in English | MEDLINE | ID: mdl-16681844

ABSTRACT

Infection of epithelial cells by the intracellular pathogen, Chlamydia trachomatis, leads to activation of NF-kappaB and secretion of pro-inflammatory cytokines. We find that overexpression of a dominant-negative Nod1 or depletion of Nod1 by RNA interference inhibits partially the activation of NF-kappaB during chlamydial infection in vitro, suggesting that Nod1 can detect the presence of Chlamydia. In parallel, there is a larger increase in the expression of pro-inflammatory genes following Chlamydia infection when primary fibroblasts are isolated from wild-type mice than from Nod1-deficient mice. The Chlamydia genome encodes all the putative enzymes required for proteoglycan synthesis, but proteoglycan from Chlamydia has never been detected biochemically. Since Nod1 is a ubiquitous cytosolic receptor for peptidoglycan from Gram-negative bacteria, our results suggest that C. trachomatis and C. muridarum do in fact produce at least the rudimentary proteoglycan motif recognized by Nod1. Nonetheless, Nod1 deficiency has no effect on the efficiency of infection, the intensity of cytokine secretion, or pathology in vaginally infected mice, compared with wild-type controls. Similarly, Rip2, a downstream mediator of Nod1, Toll-like receptor (TLR)-2, and TLR4, increases only slightly the intensity of chlamydial infection in vivo and has a very mild effect on the immune response and pathology. Thus, Chlamydia may not produce sufficient peptidoglycan to stimulate Nod1-dependent pathways efficiently in infected animals, or other receptors of the innate immune system may compensate for the absence of Nod1 during Chlamydia infection in vivo.


Subject(s)
Adaptor Proteins, Signal Transducing/physiology , Chlamydia Infections/physiopathology , Chlamydia muridarum/physiology , Chlamydia muridarum/pathogenicity , Chlamydia trachomatis/physiology , Chlamydia trachomatis/pathogenicity , Receptors, Cell Surface/physiology , Adaptor Proteins, Signal Transducing/genetics , Animals , Chlamydia Infections/pathology , Chlamydia muridarum/chemistry , Chlamydia trachomatis/chemistry , DNA, Bacterial/analysis , DNA, Bacterial/genetics , Epithelium/chemistry , Epithelium/microbiology , Epithelium/pathology , Epithelium/physiology , Female , Fibroblasts/microbiology , Fibroblasts/physiology , Gene Expression Regulation, Bacterial/physiology , HeLa Cells , Humans , Male , Mice , Mice, Inbred NOD , NF-kappa B/analysis , NF-kappa B/physiology , Nod1 Signaling Adaptor Protein , Protein Serine-Threonine Kinases/physiology , RNA, Messenger/analysis , RNA, Messenger/genetics , Receptor-Interacting Protein Serine-Threonine Kinase 2 , Receptor-Interacting Protein Serine-Threonine Kinases , Receptors, Cell Surface/analysis , Toll-Like Receptor 2/genetics , Toll-Like Receptor 2/physiology , Toll-Like Receptor 4/genetics , Toll-Like Receptor 4/physiology , Tumor Necrosis Factor Receptor-Associated Peptides and Proteins/physiology , Vagina/microbiology
5.
PLoS Pathog ; 2(5): e45, 2006 May.
Article in English | MEDLINE | ID: mdl-16710454

ABSTRACT

Chlamydiae replicate intracellularly in a vacuole called an inclusion. Chlamydial-infected host cells are protected from mitochondrion-dependent apoptosis, partly due to degradation of BH3-only proteins. The host-cell adapter protein 14-3-3beta can interact with host-cell apoptotic signaling pathways in a phosphorylation-dependent manner. In Chlamydia trachomatis-infected cells, 14-3-3beta co-localizes to the inclusion via direct interaction with a C. trachomatis-encoded inclusion membrane protein. We therefore explored the possibility that the phosphatidylinositol-3 kinase (PI3K) pathway may contribute to resistance of infected cells to apoptosis. We found that inhibition of PI3K renders C. trachomatis-infected cells sensitive to staurosporine-induced apoptosis, which is accompanied by mitochondrial cytochrome c release. 14-3-3beta does not associate with the Chlamydia pneumoniae inclusion, and inhibition of PI3K does not affect protection against apoptosis of C. pneumoniae-infected cells. In C. trachomatis-infected cells, the PI3K pathway activates AKT/protein kinase B, which leads to maintenance of the pro-apoptotic protein BAD in a phosphorylated state. Phosphorylated BAD is sequestered via 14-3-3beta to the inclusion, but it is released when PI3K is inhibited. Depletion of AKT through short-interfering RNA reverses the resistance to apoptosis of C. trachomatis-infected cells. BAD phosphorylation is not maintained and it is not recruited to the inclusion of Chlamydia muridarum, which protects poorly against apoptosis. Thus, sequestration of BAD away from mitochondria provides C. trachomatis with a mechanism to protect the host cell from apoptosis via the interaction of a C. trachomatis-encoded inclusion protein with a host-cell phosphoserine-binding protein.


Subject(s)
Chlamydia trachomatis/metabolism , Lymphogranuloma Venereum/microbiology , Lymphogranuloma Venereum/physiopathology , Vacuoles/metabolism , bcl-Associated Death Protein/metabolism , Apoptosis/drug effects , Cell Survival , Chlamydia trachomatis/physiology , Chromones/pharmacology , Cytochromes c/metabolism , Enzyme Activation , Enzyme Inhibitors/pharmacology , Epithelial Cells/metabolism , HeLa Cells , Humans , Lymphogranuloma Venereum/metabolism , Morpholines/pharmacology , Phosphatidylinositol 3-Kinases/metabolism , Phosphorylation , Proto-Oncogene Proteins c-akt/metabolism , Staurosporine/pharmacology , Tissue Distribution
6.
J Immunol ; 175(1): 450-60, 2005 Jul 01.
Article in English | MEDLINE | ID: mdl-15972679

ABSTRACT

IFN-gamma-inducible protein 10 (IP-10) is a chemokine important in the attraction of T cells, which are essential for resolution of chlamydial genital tract infection. During infections with Gram-negative bacteria, the IP-10 response mediated through type I IFNs usually occurs as a result of TLR4 stimulation by bacterial LPS. However, we found that levels of IP-10 in genital tract secretions of Chlamydia trachomatis-infected female wild-type mice were similar to those of infected TLR2- and TLR4-deficient mice but significantly greater than those of infected MyD88-deficient mice. We investigated the mechanism of IP-10 and IFN-beta induction during chlamydial infection using mouse macrophages and fibroblasts infected ex vivo. The induction of IP-10 and IFN-beta was unchanged in Chlamydia-infected TLR2- and TLR4-deficient cells compared with wild-type cells. However, infection of MyD88-deficient cells resulted in significantly decreased responses. These results suggest a role for MyD88-dependent pathways in induction of IP-10 and IFN-beta during chlamydial infection. Furthermore, treatment of infected macrophages with an endosomal maturation inhibitor significantly reduced chlamydial-induced IFN-beta. Because endosomal maturation is required for MyD88-dependent intracellular pathogen recognition receptors to function, our data suggest a role for the intracellular pathogen recognition receptor(s) in induction of IFN-beta and IP-10 during chlamydial infection. Furthermore, the intracellular pathways that lead to chlamydial-induced IFN-beta function through TANK-binding kinase mediated phosphorylation and nuclear translocation of IFN regulatory factor-3.


Subject(s)
Antigens, Differentiation/metabolism , Chemokines, CXC/biosynthesis , Chemokines, CXC/genetics , Chlamydia trachomatis/immunology , Chlamydia trachomatis/pathogenicity , Interferon-beta/biosynthesis , Interferon-beta/genetics , Receptors, Immunologic/metabolism , Adaptor Proteins, Signal Transducing , Animals , Antigens, Differentiation/genetics , Base Sequence , Chemokine CXCL10 , Chlamydia Infections/genetics , Chlamydia Infections/immunology , DNA/genetics , Endosomes/immunology , Female , Gene Expression , In Vitro Techniques , Interferon-gamma/metabolism , Macrophages/immunology , Mice , Mice, Inbred C57BL , Mice, Knockout , Myeloid Differentiation Factor 88 , RNA, Messenger/genetics , RNA, Messenger/metabolism , Receptors, Immunologic/deficiency , Receptors, Immunologic/genetics , Toll-Like Receptor 2 , Toll-Like Receptor 4
7.
Am J Physiol Gastrointest Liver Physiol ; 288(5): G1024-35, 2005 May.
Article in English | MEDLINE | ID: mdl-15662049

ABSTRACT

Extracellular nucleotides interact with purinergic receptors, which regulate ion transport in a variety of epithelia. With the use of two different human epithelial carcinoma cell lines (HCT8 and Caco-2), we have shown by RT-PCR that the cells express mRNA for P2X1, P2X3, P2X4, P2X5, P2X6, P2X7, P2Y1, P2Y2, P2Y4, P2Y6, P2Y11, and P2Y12 receptors. Protein expression for P2Y1 and P2Y2 receptors was also demonstrated immunohistochemically, and P2X receptor subtype protein was present in the following decreasing order: P2X4 > P2X7 > P2X1 > P2X3 > P2X6 > P2X5 >> P2X2. The functional presence of P2X7, P2Y1, P2Y2, and P2Y4 receptors was shown based on the effect of extracellular nucleotides on apoptosis or cell proliferation, and measurement of nucleotide-dependent calcium fluxes using a fluorometric imaging plate reader in the presence of different selective agonists and antagonists. ATP, at high concentrations, induced apoptosis through ligation of P2X7 and P2Y1 receptors; conversely, ATP, at lower concentrations, and UTP stimulated proliferation, probably acting via P2Y2 receptors. We therefore propose that stimulation or dysfunction of purinergic receptors may contribute at least partially to modulation of epithelial carcinoma cell proliferation and apoptosis.


Subject(s)
Adenosine Triphosphate/analogs & derivatives , Apoptosis/physiology , Carcinoma/pathology , Cell Proliferation , Intestinal Neoplasms/pathology , Nucleotides/physiology , Receptors, Purinergic P2/physiology , Adenosine Triphosphate/pharmacology , Apoptosis/drug effects , Calcium/pharmacology , Cell Line, Tumor , Cell Proliferation/drug effects , Gene Expression/drug effects , Humans , RNA, Messenger/metabolism , Time Factors , Zinc/pharmacology
8.
J Immunol ; 171(11): 6187-97, 2003 Dec 01.
Article in English | MEDLINE | ID: mdl-14634135

ABSTRACT

The roles of Toll-like receptor (TLR) 2 and TLR4 in the host inflammatory response to infection caused by Chlamydia trachomatis have not been elucidated. We examined production of TNF-alpha and IL-6 in wild-type TLR2 knockout (KO), and TLR4 KO murine peritoneal macrophages infected with the mouse pneumonitis strain of C. trachomatis. Furthermore, we compared the outcomes of genital tract infection in control, TLR2 KO, and TLR4 KO mice. Macrophages lacking TLR2 produced significantly less TNF-alpha and IL6 in response to active infection. In contrast, macrophages from TLR4 KO mice consistently produced higher TNF-alpha and IL-6 responses than those from normal mice on in vitro infection. Infected TLR2-deficient fibroblasts had less mRNA for IL-1, IL-6, and macrophage-inflammatory protein-2, but TLR4-deficient cells had increased mRNA levels for these cytokines compared with controls, suggesting that ligation of TLR4 by whole chlamydiae may down-modulate signaling by other TLRs. In TLR2 KO mice, although the course of genital tract infection was not different from that of controls, significantly lower levels of TNF-alpha and macrophage-inflammatory protein-2 were detected in genital tract secretions during the first week of infection, and there was a significant reduction in oviduct and mesosalpinx pathology at late time points. TLR4 KO mice responded to in vivo infection similarly to wild-type controls and developed similar pathology. TLR2 is an important mediator in the innate immune response to C. trachomatis infection and appears to play a role in both early production of inflammatory mediators and development of chronic inflammatory pathology.


Subject(s)
Chlamydia Infections/immunology , Chlamydia trachomatis , Fallopian Tubes/pathology , Genital Diseases, Female/immunology , Membrane Glycoproteins/physiology , Receptors, Cell Surface/physiology , Animals , Antibodies, Bacterial/blood , Cells, Cultured , Chemokine CXCL2 , Chemokines/genetics , Chemokines/metabolism , Chlamydia Infections/genetics , Chlamydia Infections/pathology , Down-Regulation/genetics , Down-Regulation/immunology , Fallopian Tubes/immunology , Female , Fibroblasts/immunology , Fibroblasts/microbiology , Fibroblasts/pathology , Genital Diseases, Female/genetics , Genital Diseases, Female/pathology , Immunoglobulin G/blood , Interleukin-1/genetics , Interleukin-1/metabolism , Interleukin-6/genetics , Interleukin-6/metabolism , Lung/immunology , Lung/microbiology , Lung/pathology , Macrophages, Peritoneal/immunology , Macrophages, Peritoneal/metabolism , Membrane Glycoproteins/deficiency , Membrane Glycoproteins/genetics , Mice , Mice, Inbred C57BL , Mice, Knockout , Pneumonia/genetics , Pneumonia/immunology , RNA, Messenger/antagonists & inhibitors , RNA, Messenger/metabolism , Receptors, Cell Surface/deficiency , Receptors, Cell Surface/genetics , Toll-Like Receptor 2 , Toll-Like Receptor 4 , Toll-Like Receptors , Tumor Necrosis Factor-alpha/metabolism
9.
Biochimie ; 85(8): 763-9, 2003 Aug.
Article in English | MEDLINE | ID: mdl-14585543

ABSTRACT

Infections by Chlamydia are followed by a strong inflammatory response, which is necessary to eliminate the infection, but at the same time is responsible for the pathology of infection. Resistance of infected cells against apoptosis induced by external ligands, together with the effects of IFNgamma secreted during infection, would be expected to contribute to persistence of infection. Secretion of TNFalpha plays an important role during clearance of the chlamydiae, but also triggers apoptosis of uninfected cells in infected tissues. Apoptosis of infected host-cells towards the end of the infection cycle is thought to participate in the release of chlamydiae from infected cells and propagation of the infection. Dysregulation of the apoptotic program during infection leads to a less efficient infection, but paradoxically, results in a higher inflammatory response and more severe pathology.


Subject(s)
Chlamydia Infections/pathology , Chlamydia/pathogenicity , Inflammation/etiology , Proto-Oncogene Proteins c-bcl-2 , Apoptosis/physiology , Cell Death/physiology , Chlamydia Infections/complications , Chlamydia Infections/microbiology , Cytokines/metabolism , Humans , Inflammation/microbiology , Inflammation/pathology , Interferon-gamma/metabolism , Proto-Oncogene Proteins/genetics , Proto-Oncogene Proteins/metabolism , Tumor Necrosis Factor-alpha/metabolism , bcl-2-Associated X Protein
10.
Immunity ; 19(3): 403-12, 2003 Sep.
Article in English | MEDLINE | ID: mdl-14499115

ABSTRACT

Chlamydia trachomatis survives within host cells by inhibiting fusion between Chlamydia vacuoles and lysosomes. We show here that treatment of infected macrophages with ATP leads to killing of chlamydiae through ligation of the purinergic receptor, P2X(7)R. Chlamydial killing required phospholipase D (PLD) activation, as PLD inhibition led to rescue of chlamydiae in ATP-treated macrophages. However, there was no PLD activation nor chlamydial killing in ATP-treated P2X(7)R-deficient macrophages. P2X(7)R ligation exerts its effects by promoting fusion between Chlamydia vacuoles and lysosomes. P2X(7)R stimulation also resulted in macrophage death, but fusion with lysosomes preceded macrophage death and PLD inhibition did not prevent macrophage death. These results suggest that P2X(7)R ligation leads to PLD activation, which is directly responsible for inhibition of infection.


Subject(s)
Chlamydia Infections/metabolism , Phospholipase D/metabolism , Receptors, Purinergic P2/metabolism , Adenosine Triphosphate/metabolism , Animals , Chlamydia Infections/immunology , Chlamydia trachomatis/immunology , Lysosomes/metabolism , Macrophages/immunology , Macrophages/microbiology , Mice , Receptors, Purinergic P2X7
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