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1.
Sci Rep ; 10(1): 12479, 2020 07 27.
Article in English | MEDLINE | ID: mdl-32719358

ABSTRACT

The arrangement and composition of habitats within landscapes and fine-scale habitat characteristics influence community structure and ecological processes. These aspects can be altered by anthropogenic activities, thus influencing associated assemblages. Farming of macroalgae is a common practice in tropical settings and alters the natural composition of seascapes by introducing monoculture patches. The farmed macroalgae may also differ in palatability compared to naturally-occurring macroalgae, influencing herbivory. This study assessed how these farms may differ from natural macroalgal beds in terms of habitat heterogeneity, fish assemblages, and herbivory. We surveyed fish assemblages and deployed macroalgal assays within macroalgal beds, farms and at varying distances from these habitats near Mafia Island, Tanzania. Fish composition and herbivory differed between the habitats likely due to different macrophyte species richness, underlying hard substrate in natural macroalgal beds, and high abundance of browsers nearby the farms. Additionally, fish assemblage patterns and herbivory were not consistent across the seascapes and varied with distance from the focal habitats possibly due to the presence of other habitats. The results suggest alterations of seascapes by farming practices may have consequences on fish assemblages and the ecological functions performed, thus positioning of farms should be carefully considered in management and conservation plans.


Subject(s)
Ecosystem , Fishes/classification , Animals , Fishes/genetics , Fishes/growth & development , Indian Ocean , Seaweed/growth & development , Tanzania
2.
Acta Psychiatr Scand ; 137(5): 371-379, 2018 05.
Article in English | MEDLINE | ID: mdl-29603145

ABSTRACT

OBJECTIVE: To identify the determinants of natural cause mortality in a cohort of individuals with serious mental illness assessed prospectively. METHOD: Persons with schizophrenia (n = 789) and bipolar disorder (n = 498), mean age of 38 (s.d. 12.6) years, underwent an in-person clinical assessment. They also had a blood sample drawn from which infectious disease markers were measured. Mortality was subsequently determined utilizing data from the National Death Index following a period of up to 16.9 years. RESULTS: A total of 6.8% (87 of 1287) of persons died of natural causes. Mortality was predicted in a multivariate model by baseline cigarette smoking (RR = 6.29, 95% CI 1.41, 3.72, P = 0.00076); divorced or widowed status (RR = 1.90, CI 1.21, 2.99); reduced cognitive score (RR = 0.73, CI 0.61, 0.87); receipt of antidepressant medication (RR = 1.74, CI 1.12, 2.71); elevated levels of antibodies to Epstein-Barr virus (EBV) (RR = 1.29, CI 1.01, 1.66); and a genitourinary (RR = 1.82, CI 1.16, 2.86), respiratory (RR = 1.82, CI 1.16, 2.86), or cardiac (RR = 2.09, CI 1.33, 3.29) condition. There was an additive effect of smoking and both a cardiac and a respiratory condition but not elevated EBV antibody levels. CONCLUSION: Smoking is a modifiable behaviour which is associated with mortality in this population.


Subject(s)
Bipolar Disorder/epidemiology , Cause of Death , Cigarette Smoking/epidemiology , Heart Diseases/epidemiology , Lung Diseases/epidemiology , Psychotic Disorders/epidemiology , Schizophrenia/epidemiology , Adult , Comorbidity , Female , Follow-Up Studies , Humans , Male , Middle Aged , Risk
3.
Clin Microbiol Infect ; 24(9): 1019.e5-1019.e8, 2018 Sep.
Article in English | MEDLINE | ID: mdl-29496594

ABSTRACT

OBJECTIVES: A carbapenem-resistant Providencia rettgeri (PR1) isolate was recovered from a wound infection in Missouri, USA. This isolate possessed an EDTA-inhibitable carbapenemase that was unidentified using the Xpert CARBA-R assay. Our objective was to elucidate the molecular determinant of carbapenem resistance in this isolate. We then sought to test the transmissibility of blaIMP-27 loci in clinical P. rettgeri and Proteus mirabilis isolates. METHODS: In October 2016 the novel ambler Class B carbapenemase blaIMP-27, was reported in two different Proteus mirabilis (PM185 and PM187) isolates. Broth mating assays for transfer of carbapenemase activity were performed for the three clinical isolates with recipient sodium azide-resistant Escherichia coli J53. Antibiotic susceptibility testing and phenotypic carbapenemase activity testing were performed on the clinical isolates, J53 and transconjugants using the Kirby-Bauer disc diffusion method according to CLSI guidelines. Plasmid DNA from PM187, PR1 and their transconjugants were used as input for Nextera Illumina sequencing libraries and sequenced on a NextSeq platform. RESULTS: PR1 was resistant to both imipenem and meropenem. PM187 and PR1 could transfer resistance to E. coli through plasmid conjugation (pPM187 and pPR1). pPM187 had a virB/virD4 type IV secretion system whereas pPR1 had a traB/traD type IV secretion system. CONCLUSION: Two of three blaIMP-27-bearing clinical isolates tested could conjugate resistance into E. coli. The resulting transconjugants became positive for phenotypic carbapenemase production but did not pass clinical resistance breakpoints. blaIMP-27 can be transmitted on different plasmid replicon types that rely on distinct classes of type IV secretion system for horizontal transfer.


Subject(s)
Bacterial Proteins/genetics , Gene Transfer, Horizontal , Plasmids/genetics , Proteus mirabilis/genetics , Providencia/genetics , beta-Lactamases/genetics , Anti-Bacterial Agents/pharmacology , Disk Diffusion Antimicrobial Tests , High-Throughput Nucleotide Sequencing , Humans , Imipenem/pharmacology , Meropenem , Plasmids/metabolism , Proteus mirabilis/drug effects , Providencia/drug effects , Sequence Analysis, DNA , Thienamycins/pharmacology
4.
Transl Psychiatry ; 7(8): e1186, 2017 08 01.
Article in English | MEDLINE | ID: mdl-28763062

ABSTRACT

Autoantibodies that bind the N-methyl-D-aspartate receptor (NMDAR) may underlie glutamate receptor hypofunction and related cognitive impairment found in schizophrenia. Exposure to neurotropic pathogens can foster an autoimmune-prone environment and drive systemic inflammation leading to endothelial barrier defects. In mouse model cohorts, we demonstrate that infection with the protozoan parasite, Toxoplasma gondii, caused sustained elevations of IgG class antibodies to the NMDAR in conjunction with compromised blood-gut and blood-brain barriers. In human cohorts, NMDAR IgG and markers of barrier permeability were significantly associated with T. gondii exposure in schizophrenia compared with controls and independently of antipsychotic medication. Combined T. gondii and NMDAR antibody seropositivity in schizophrenia resulted in higher degrees of cognitive impairment as measured by tests of delayed memory. These data underscore the necessity of disentangling the heterogeneous pathophysiology of schizophrenia so that relevant subsets eligible for NMDAR-related treatment can be identified. Our data aid to reconcile conflicting reports regarding a role of pathological NMDAR autoantibodies in this disorder.


Subject(s)
Autoantibodies/immunology , Receptors, N-Methyl-D-Aspartate/immunology , Schizophrenia/immunology , Adult , Animals , Autoimmunity , Female , Humans , Male , Mice , Middle Aged , Toxoplasma/immunology , Young Adult
5.
J Fish Biol ; 90(2): 611-625, 2017 Feb.
Article in English | MEDLINE | ID: mdl-27680502

ABSTRACT

Chemical properties of fin rays were investigated in nine fish species to test whether life-history characteristics can be analysed using a non-lethal and minimally invasive methodology. Fish specimens from public aquariums were acquired after fishes died in captivity. Analyses concentrated on exploring the differences between the wild and captive life periods of each fish, which were known from aquarium records. Differences between the two life periods were observed in both the trace-element and stable-isotope compositions of the chemical matrix of the fin ray. Trace-element concentrations in fin rays were compared with those in otoliths using measures of resolved variance and cross-correlation to test the assumption of conserved matrices in the fin ray. Divalent ions and positively charged transition metals (i.e. Fe and Co) had strong associations between the two structures, suggesting conservation of material. Stable-isotope values of δ13 C and δ15 N differed between the wild and captive life periods in most of the fishes, also suggesting conserved matrices. δ13 C and δ15 N were derived from the organic matrix within the fin ray, which may present a stable-isotope chronology. Future studies can use these chronologies to study diet and movement trends on a temporal scale consistent with the entire lifetime of an individual.


Subject(s)
Animal Fins/chemistry , Fishes , Otolithic Membrane/chemistry , Animal Distribution , Animals , Carbon Isotopes/analysis , Diet , Feeding Behavior , Nitrogen Isotopes/analysis , Trace Elements/analysis
6.
Prog Mol Biol Transl Sci ; 143: 1-48, 2016.
Article in English | MEDLINE | ID: mdl-27697200

ABSTRACT

The pituitary gonadotrope is central to reproductive function. Gonadotropes develop in a systematic process dependent on signaling factors secreted from surrounding tissues and those produced within the pituitary gland itself. These signaling pathways are important for stimulating specific transcription factors that ultimately regulate the expression of genes and define gonadotrope identity. Proper gonadotrope development and ultimately gonadotrope function are essential for normal sexual maturation and fertility. Understanding the mechanisms governing differentiation programs of gonadotropes is important to improve treatment and molecular diagnoses for patients with gonadotrope abnormalities. Much of what is known about gonadotrope development has been elucidated from mouse models in which important factors contributing to gonadotrope development and function have been deleted, ectopically expressed, or modified. This chapter will focus on many of these mouse models and their contribution to our current understanding of gonadotrope development.


Subject(s)
Gonadotrophs/cytology , Animals , Gonadotrophs/metabolism , Integrases/metabolism , Mice , Models, Animal , Signal Transduction , Transcription Factors/metabolism
7.
Acta Psychiatr Scand ; 132(3): 204-10, 2015 Sep.
Article in English | MEDLINE | ID: mdl-26061032

ABSTRACT

OBJECTIVE: Immunologic abnormalities have been found in bipolar disorder and acute mania. However, there have been fewer studies of patients with acute bipolar depression. METHOD: Blood samples were obtained from individuals with acute bipolar depression, acute mania, and controls. These samples were evaluated for antibodies to human herpesviruses, gliadin, Toxoplasma gondii, and endogenous retroviruses as well as for C-reactive protein (CRP) and pentraxin-3 using immunoassay methods. Linear regression models were used to compare the levels of the markers controlling for demographic and clinical variables. A subset of the bipolar depressed group was evaluated at a 6-month follow-up. RESULTS: The sample consisted of 82 individuals with acute bipolar depression, 147 with acute mania, and 280 controls. The levels of CRP and IgG antibodies to an endogenous retrovirus, Mason-Pfizer monkey virus (MPMV), were significantly elevated in the bipolar depressed group. Levels of pentraxin-3 were reduced in both psychiatric groups. An evaluation of 32 individuals 6 months after hospitalization for bipolar depression showed a significant decrease in the levels of MPMV antibodies, but not a change in the other markers. CONCLUSION: Individuals with acute bipolar depression show immune alterations. Some of the alterations are similar to those found in acute mania.


Subject(s)
Bipolar Disorder/immunology , Acute Disease , Adult , Biomarkers/blood , Bipolar Disorder/blood , Bipolar Disorder/parasitology , Bipolar Disorder/virology , C-Reactive Protein/immunology , Female , Follow-Up Studies , Humans , Male , Middle Aged , Neuroimmunomodulation , Serum Amyloid P-Component/immunology
8.
Oecologia ; 179(3): 729-39, 2015 Nov.
Article in English | MEDLINE | ID: mdl-26093629

ABSTRACT

Many species have multi-stage life cycles in which the youngest stages (e.g., larvae) are small, dispersive, and abundant, whereas later stages are sessile or sedentary. Quantifying survival throughout such early stages is critical for understanding dispersal, population dynamics, and life history evolution. However, dispersive stages can be very difficult to sample in situ, and estimates of survival through the entire duration of these stages are typically poor. Here we describe how demographic information from juveniles and adults can be used to estimate survival throughout a dispersive larval stage that was not sampled directly. Using field measurements of demography, we show that detailed information on post-settlement growth, survival, and reproduction can be used to estimate average larval survivorship under the assumption that a typical individual replaces itself over its lifetime. Applying this approach to a common coral reef fish (bicolor damselfish, Stegastes partitus), we estimated average larval survivorship to be 0.108% (95% CI 0.025-0.484). We next compared this demography-based estimate to an expected value derived from published estimates of larval mortality rates. Our estimate of larval survivorship for bicolor damselfish was approximately two orders of magnitude greater than what would be expected if larval mortality of this species followed the average, size-dependent pattern of mortality inferred from a published sample of marine fishes. Our results highlight the importance of understanding mortality during the earliest phases of larval life, which are typically not sampled, as well as the need to understand the details of how larval mortality scales with body size.


Subject(s)
Coral Reefs , Fishes/growth & development , Animal Distribution , Animals , Biological Evolution , Body Size , Conservation of Natural Resources , Fishes/physiology , Larva/growth & development , Larva/physiology , Life Cycle Stages , Population Density , Population Dynamics , Reproduction
9.
J Dairy Sci ; 95(12): 7261-8, 2012 Dec.
Article in English | MEDLINE | ID: mdl-23040023

ABSTRACT

Milk urea nitrogen (MUN) is correlated with N balance, N intake, and dietary N content, and thus is a good indicator of proper feeding management with respect to protein. It is commonly used to monitor feeding programs to achieve environmental goals; however, genetic diversity also exists among cows. It was hypothesized that phenotypic diversity among cows could bias feed management decisions when monitoring tools do not consider genetic diversity associated with MUN. The objective of the work was to evaluate the effect of cow and herd variation on MUN. Data from 2 previously published research trials and a field trial were subjected to multivariate regression analyses using a mixed model. Analyses of the research trial data showed that MUN concentrations could be predicted equally well from diet composition, milk yield, and milk components regardless of whether dry matter intake was included in the regression model. This indicated that cow and herd variation could be accurately estimated from field trial data when feed intake was not known. Milk urea N was correlated with dietary protein and neutral detergent fiber content, milk yield, milk protein content, and days in milk for both data sets. Cow was a highly significant determinant of MUN regardless of the data set used, and herd trended to significance for the field trial data. When all other variables were held constant, a percentage unit change in dietary protein concentration resulted in a 1.1mg/dL change in MUN. Least squares means estimates of MUN concentrations across herds ranged from a low of 13.6 mg/dL to a high of 17.3 mg/dL. If the observed MUN for the high herd were caused solely by high crude protein feeding, then the herd would have to reduce dietary protein to a concentration of 12.8% of dry matter to achieve a MUN concentration of 12 mg/dL, likely resulting in lost milk production. If the observed phenotypic variation is due to genetic differences among cows, genetic choices could result in herds that exceed target values for MUN when adhering to best management practices, which is consistent with the trend for differences in MUN among herds.


Subject(s)
Cattle/metabolism , Milk/chemistry , Urea/analysis , Animals , Diet/veterinary , Eating , Female , Lactation/metabolism , Nitrogen/analysis
10.
Environ Sci Technol ; 35(5): 845-55, 2001 Mar 01.
Article in English | MEDLINE | ID: mdl-11351526

ABSTRACT

Despite tremendous efforts toward regulating and controlling tropospheric ozone (O3) formation, a large portion of the U.S. population presently lives in environments where air quality exceeds both 1- and 8-h National Ambient Air Quality Standards (NAAQS) set for O3. High O3 concentrations annually cost the United States billions of dollars in excessive human health costs, reduced crop yields, and ecological damage. This paper describes a regional networking of O3 monitoring sites, operated by the public, that used simplified passive sampling devices (PSDs). In collaboration with EPA Region 6, a lay network (i.e., Passive Ozone Network of Dallas, acronym POND), consisting of 30 PSD sites in the Dallas-Fort Worth (DFW) Metroplex, a region representing 16 counties, successfully measured daily ozone during 8 weeks of the 1998 high ozone season. It was demonstrated that the concerned public, when properly trained, could successfully operate a large PSD network that requires daily sample handling and weekly mailing procedures, even from remote sites. Data treatment of the 2880 POND measurements included (i) high correlations with collocated continuous monitoring data [r range = 0.95-0.97], (ii) daily O3 contour mapping of the 24,000 km2 area, and (iii) a ranking of O3 severity in 12 peri-urban counties for guidance in sitting additional monitors. With a new 8-h NAAQS standard now in place, a cost-effective network such as POND could aid regional airshed models in generating meaningful guidance for O3 state implementation plans (SIPs) by providing input that is representative of both rural and urban sites.


Subject(s)
Community Participation , Environmental Monitoring/methods , Oxidants, Photochemical/analysis , Ozone/analysis , Adult , Child , Cities , Education , Humans , Texas , Volunteers
11.
Environ Sci Technol ; 35(22): 4426-35, 2001 Nov 15.
Article in English | MEDLINE | ID: mdl-11757597

ABSTRACT

Attaining the current lower tropospheric U.S. ozone standards continues to be a difficult task for many areas in the U.S. Concentrations of ozone above the standards negatively affects human health, agricultural crops, forests, and other ecosystem elements. This paper describes year two (1999) of a regional networking of passive and continuous ozone monitoring sites in the Dallas-Fort Worth (DFW) Metroplex region. The objectives of the second year of study were to (1) validate conclusions of the 1998 Passive Ozone Network of Dallas (POND) I study, (2) define the value of taking 12-h diurnal samples in addition to 24-h samples, and (3) add to the scientific knowledge base of rural/urban ozone comparison studies. Results of the POND II (1999) study demonstrated that ozone concentrations exceeding the new 8-h ozone standard could be recorded at least 130 km, or 80 miles, from the DFW Metroplex core in more rural areas. In addition, results of the POND II study indicated that ozone concentrations exceeding the 8-h standard probably occurred in areas recording a 12-h daytime ozone concentration above 60 parts per billion (ppb). The 12-h passive ozone data from POND II also suggests the relative magnitude of anthropogenic pollution influence could be assessed for rural passive ozone sites. The data from the POND II study provide modelers a rich database for future photochemical subgrid development for the DFW ozone nonattainment area. Indeed, the POND database provides a great amount of additional ozone ambient data covering 26 8-h and 13 1-h ozone standard exceedance days over an approximate 25000 km2 region. These data should help decrease uncertainties derived from future DFW ozone model exercises.


Subject(s)
Air Pollutants/analysis , Databases, Factual , Environmental Monitoring/methods , Models, Theoretical , Oxidants, Photochemical/analysis , Ozone/analysis , Cities , Community-Institutional Relations , Geography , Humans , Public Health , Reference Values , Texas
12.
Am J Vet Res ; 61(9): 1052-6, 2000 Sep.
Article in English | MEDLINE | ID: mdl-10976735

ABSTRACT

OBJECTIVE: To determine effects of breed and supplemental administration of vitamin E and selenium (Se) during late gestation on circulating concentrations of these micronutrients in periparturient Jerseys and Holsteins. DESIGN: Randomized controlled clinical study. ANIMALS: 16 Jersey and 36 Holstein cows. PROCEDURE: Cows were allotted to blocks on the basis of breed and expected parturition date. Cows within blocks were randomly assigned to be given vitamin E or Se parenterally 3 to 4 weeks prior to anticipated parturition in a 2 x 2 factorial design. RESULTS: Results of ANOVA indicated Jerseys had higher blood concentrations of Se and lower serum concentrations of vitamin E than Holsteins at the end of lactation. Jerseys had higher blood concentrations of Se than Holsteins 3 to 4 weeks prior to parturition and at parturition. Selenium administration increased blood concentrations of Se at parturition. Administration of nutrients did not affect serum concentrations of vitamin E at parturition or 2 to 3 weeks after parturition or blood concentrations of Se 2 to 3 weeks after parturition. CONCLUSIONS AND CLINICAL RELEVANCE: Jerseys and Holsteins consuming rations of comparable Se content differ in blood concentrations of Se during the nonlactating period, suggesting breed-related differences in Se metabolism during late lactation and the nonlactating period. Parenteral administration of Se 3 to 4 weeks prior to anticipated parturition increased blood concentrations of Se at parturition; however, Se concentrations of both groups at parturition were considered within the reference range for clinically normal cattle.


Subject(s)
Cattle/metabolism , Labor, Obstetric/drug effects , Lactation/drug effects , Selenium/pharmacology , Vitamin E/pharmacology , Animals , Cattle/classification , Dietary Supplements , Female , Infusions, Parenteral/veterinary , Pregnancy , Selenium/administration & dosage , Selenium/blood , Vitamin E/administration & dosage , Vitamin E/blood
13.
J Dairy Sci ; 81(5): 1353-7, 1998 May.
Article in English | MEDLINE | ID: mdl-9621238

ABSTRACT

A microbial and enzyme supplement fed at 21.2 g/d per cow to 46 Virginia dairy herds increased the milk yield of 31 herds (17 significantly) and decreased the milk yield of 15 herds (7 significantly). Effects of season were important but consistent with overall results. Herds began receiving the supplement, which contained dried fermentation products of Aspergillus oryzae, Bacillus subtilis, Lactobacillus acidophilus, and yeast culture, midway between the first and second monthly Dairy Herd Improvement tests and continued on the supplement through the 3rd mo. Entry of herds was staggered over 8 mo to reduce the influence of season. The trial involved 3417 cows with 5 test mo between 60 and 365 d in milk. Milk yield during mo 3 averaged 0.64 kg/d per cow more (+0.73 kg/d for first lactation cows and +0.56 kg/d for later lactation cows) than the mean milk yield during mo 1 and 5. Herds completing the study before summer responded similarly to all other herds, which included herds that were fed the product during summer and those that finished the study during summer. Fat and protein yields and protein percentage differed little with or without the supplement. Fat percentage decreased (0.10%). Twenty-one herds that were fed a yeast product prior to and during the study responded similarly to the 17 herds that were not fed a yeast product.


Subject(s)
Bacteria , Cattle/physiology , Lactation/physiology , Probiotics , Saccharomyces cerevisiae , Animals , Aspergillus oryzae , Bacillus subtilis , Cellulase/administration & dosage , Female , Fermentation , Glucan Endo-1,3-beta-D-Glucosidase/administration & dosage , Glycoside Hydrolases/administration & dosage , Lactobacillus acidophilus , alpha-Amylases/administration & dosage
14.
J Dairy Sci ; 80(2): 353-63, 1997 Feb.
Article in English | MEDLINE | ID: mdl-9058278

ABSTRACT

Twelve Holstein cows and 12 Jersey cows were used in six 4 x 4 Latin squares to investigate the effects of the degradability of dietary protein and supplemental dietary fat on milk components. Dietary dry matter contained 16% crude protein with two concentrations of ruminally undegradable protein (RUP) obtained by substituting blood meal for a portion of the soybean meal. Treatments were 1) 29% RUP, 0% added fat; 2) 29% RUP, 2.7% added fat (Ca soaps of fatty acids); 3) 41% RUP, 0% added fat; and 4) 41% RUP, 2.7% added fat. The dry matter of the total mixed ration fed at 1000 and 1400 h consisted of 30% corn silage, 29% alfalfa haylage, and 41% concentrate. Supplemental dietary fat depressed dry matter intake by 6.2%. Plasma urea N was greater at 0700 and 1600 h for Jerseys fed diets containing added fat and greater at 0700 h for Holsteins fed diets containing 41% RUP than for Holsteins fed 0% added fat and 29% RUP. When averaged across both breeds, milk production increased 7.1%, and production of 4% fat-corrected milk by Jerseys increased 8.4%, in response to added dietary fat. Milk protein was reduced when Holstein diets contained 41% RUP. Milk protein content was reduced 7.1 and 3.9%, and milk urea N was increased 4.9 and 8.5%, by added fat and 41% RUP in both breeds, respectively. Added fat reduced the concentration, but not the yield, of milk components. Substitution of blood meal decreased the concentration and yield of milk protein and casein N.


Subject(s)
Cattle/physiology , Dietary Fats/metabolism , Dietary Proteins/metabolism , Milk/metabolism , Rumen/metabolism , Animals , Blood , Blood Urea Nitrogen , Caseins/metabolism , Diet , Dietary Fats/administration & dosage , Dietary Proteins/administration & dosage , Eating , Female , Hydrogen-Ion Concentration , Lactation , Milk Proteins/metabolism , Nitrogen/metabolism , Glycine max , Species Specificity , Urea/metabolism
15.
J Dairy Sci ; 80(12): 3368-76, 1997 Dec.
Article in English | MEDLINE | ID: mdl-9436119

ABSTRACT

Four Holstein and four Jersey cows fitted with ruminal and duodenal cannulas were used in two 4 x 4 Latin squares to investigate the effects of varying protein degradability and supplemental fat on diurnal changes in plasma and milk urea N. Dietary dry matter contained 16.2% crude protein with two concentrations of ruminally undegradable protein (RUP) that were obtained by substituting blood meal for a portion of soybean meal. Treatments were 1) 29% RUP and 0% added fat, 2) 29% RUP and 2.7% added fat (Ca soaps of fatty acids), 3) 41% RUP and 0% added fat, and 4) 41% RUP and 2.7% added fat. Dry matter of the total mixed diet fed at 1000 and 1400 h consisted of 30% corn silage, 29% alfalfa haylage, and 41% concentrate. Ruminal ammonia, plasma urea N, and milk urea N were measured every 4 h over a 24-h period. Dry matter intake was depressed 6.7% by added fat. Ruminal ammonia was 25 to 45% lower when the 41% RUP diets were fed. Overall, the concentration of plasma urea N and milk components were not influenced by diet. However, milk urea N was higher in Holsteins than in Jerseys. Both plasma and milk urea N increased within 2 h after the 1000-h feeding followed by a decline at 6 h after the 1400-h feeding. In this short-term study, fat supplementation had no effect on milk production or yields of milk components. The inclusion of blood meal, however, increased the yields of milk components. Plasma and milk urea N did not differ among dietary treatments but varied throughout the day in relation to the time of feeding.


Subject(s)
Blood Urea Nitrogen , Cattle/physiology , Circadian Rhythm , Milk/metabolism , Nitrogen/metabolism , Urea/metabolism , Ammonia/metabolism , Animal Feed , Animals , Dietary Fats/administration & dosage , Dietary Proteins/administration & dosage , Female , Hydrogen-Ion Concentration , Lactation , Medicago sativa , Rumen/metabolism , Silage , Zea mays
16.
J Dairy Sci ; 76(4): 1074-82, 1993 Apr.
Article in English | MEDLINE | ID: mdl-8387550

ABSTRACT

A trial was designed to study the relationships of dietary fiber and protein percentage and source to fecal consistency in lactating cattle. Thirty Holstein cows were assigned randomly to one of six TMR through four 21-d periods. The TMR were formulated to contain 17 or 25% ADF and CP of 15 or 22% with soybean meal supplementation or 22% with a combination of corn gluten and soybean meals. Two forage combinations were corn silage with or without alfalfa. Fecal consistency was evaluated using a four-point visual observation scale. Lower dietary fiber reduced fecal pH, score, NDF, and ADF but increased fecal DM and starch. A higher percentage of soybean meal lowered fecal DM and fecal score. Forage source affected fecal DM, NDF, ADF, and starch, but not pH or score. Prediction of fecal score from dietary components and cow parameters resulted in dietary DM percentage and 4% FCM as the most related variables. Accurate prediction of fecal consistency score from dietary and cow parameters was not possible.


Subject(s)
Cattle , Diet , Feces/chemistry , Lactation , Animals , Dietary Fiber/administration & dosage , Dietary Proteins/administration & dosage , Female , Hydrogen-Ion Concentration , Medicago sativa , Milk Proteins/analysis , Silage , Starch/analysis , Zea mays
18.
J Dairy Sci ; 67(4): 902-7, 1984 Apr.
Article in English | MEDLINE | ID: mdl-6547154

ABSTRACT

Lead factors are used in computerized ration formulation programs developed at Virginia Tech to increase milk production above a herd or group average for which total mixed rations are formulated for group feeding. These lead factors theoretically increase milk production for which the ration is formulated so 83% of the cows in a group will receive adequate or more than adequate nutrients from the ration. Two methods of calculating lead factors produced similar results. When test-day Dairy Herd Improvement records within a herd were not grouped by production, the average lead factor was 1.31 for one method [lead factor = (mean milk yield + one standard deviation)/mean milk yield] and 1.32 for the other [lead factor = (milk yield of 83rd percentile cow)/mean milk yield]. Grouping test-day records in each herd by milk production resulted in smaller lead factors for each group compared with lead factors for ungrouped herds. Changes of percentage of cows in each group in a herd resulted in different lead factors. Generally, groups with greater proportion of cows had larger lead factors. Season, herd size, and herd milk production slightly affected lead factors, but little improvement of estimation was gained by considering these variables.


Subject(s)
Animal Feed , Cattle/metabolism , Milk/metabolism , Animals , Female , Lactation , Seasons , Software
19.
N C Med J ; 45(1): 47-51, 1984 Jan.
Article in English | MEDLINE | ID: mdl-6366580
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