ABSTRACT
Lymphocytes isolated from the blood of healthy individuals (analyzed on Parmoquant-2 apparatus) showed mean electrophoretic mobility (EM) of 1.00 +/- 0.05 um sec-1 V-1 cm and the histogram appeared to be an asymmetric curve - sum of B and T lymphocytes subpopulation. In 11 patients with chronic lymphatic leukemia (CLL) and in 4 with acute lymphatic leukemia (ALL) the histogram showed a domination of one of the population. On the other hand, in patients with chronic myeloid leukemia (CML) the histograms seem to be a sum of various populations of different mobility which was illustrated by curves of a long base and several peaks.
Subject(s)
B-Lymphocytes/cytology , Leukemia/pathology , T-Lymphocytes/cytology , Electrophoresis/methods , Humans , Leukapheresis/methods , Leukemia, Lymphoid/pathology , Leukemia, Myeloid, Acute/pathology , Reference ValuesABSTRACT
In a prospective study 100 AML cases were randomized in two groups and underwent remission induction treatment in 4 departments with "Ara-C c. inf. from the first to the eight day plus Adr. from the first to the third day" or "Ara-C + 6-TG + Adr., 6 + 6 + 2 days". The CR patients were divided into two subgroups. Subgroup A obtained maintenance therapy with 5 day course of Ara-C s.c. in addition to 6-TG, Vincristine plus Prednisone and Adriamycin sequentially, with each cycle repeated at 6 week intervals. Subgroup B obtained the calf thymus extract (TFX-Polfa) between the cycles additionally. The current results of remission induction treatment are similar to "8 + 3" and "6 + 6" regimens as measured by the CR rate (45% and 44%), survival curves (survival after 24 months - 23% and 28% respectively) and relapse rates (3.5%/pt.mo.obs. and 5.3%/pt.mo.obs.). It is still too early to evaluate the efficacy of this maintenance treatment, because the programme is still open to patient entries. At present 13 patients received maintenance regimen A and 12 patients received maintenance regimen B, with an average survival being equal to 14 and 13.5 months respectively.
Subject(s)
Leukemia, Myeloid, Acute/therapy , Thioguanine/therapeutic use , Tissue Extracts/therapeutic use , Adult , Animals , Blood Transfusion , Cattle/immunology , Cytarabine/therapeutic use , Doxorubicin/therapeutic use , Granulocytes/transplantation , Humans , Leukemia, Myeloid, Acute/drug therapy , Middle Aged , Platelet Transfusion , Poland , Prospective Studies , Random Allocation , Thymus Gland/immunologyABSTRACT
To determine the influence of low temperature (-196 degrees C) during preservation upon the electrophoretic mobility (EM) of lymphocytes ten healthy donors were examined. Lymphocytes were isolated from venous blood by Ficoll-Uropolinum (Polfa) density gradient centrifugation and iron powder technique was used to eliminate macrophages. EM of lymphocytes was analyzed on Parmoquant 2 computer system (Carl-Zeiss, Jena) before and ten days after cryopreservation. There was no significant difference between the mean EM before freezing (1.046 um.sec-1.v-1.cm) and after it (1.034 um.sec.-1v-1 cm). No significant changes in histograms showing slow (lymphocytes B) and fast (lymphocytes T) fractions were observed. It indicates, that cryopreservation does not change EM of lymphocytes and thus can be used in situations, when immediate examination cannot be done.
