ABSTRACT
The paper shows it possible to use stained polymeric microspheres, 1.7 microm in diameter, that contain viruses onto the surface, in the latex agglutination test to detect antibodies to the bovine serum viruses of infective rhinotracheitis, parainfluenza-3, viral diarrhea, respiratory syncytial infection, and adenoviral infection.
Subject(s)
Adenoviridae Infections/veterinary , Antibodies, Viral/blood , Cattle Diseases/blood , Infectious Bovine Rhinotracheitis/diagnosis , Latex Fixation Tests/veterinary , Microspheres , Respirovirus Infections/veterinary , Virus Diseases/veterinary , Viruses/metabolism , Adenoviridae/immunology , Adenoviridae/metabolism , Adenoviridae Infections/blood , Adenoviridae Infections/diagnosis , Animals , Cattle , Cattle Diseases/diagnosis , Herpesviridae/immunology , Herpesviridae/metabolism , Infectious Bovine Rhinotracheitis/blood , Latex Fixation Tests/methods , Parainfluenza Virus 3, Bovine/immunology , Parainfluenza Virus 3, Bovine/metabolism , Polymers/metabolism , Respirovirus Infections/blood , Respirovirus Infections/diagnosis , Suspensions/metabolism , Virus Diseases/blood , Virus Diseases/diagnosis , Viruses/immunologyABSTRACT
Phagocytosis of polymer microspheres both modified and unmodified by bioligands (gelatin, fibronectin and A protein) by leukocytic blood cells of patients with acute pancreatitis. The presence of bioligands in the surface of polymer microspheres and their activity were monitored by the reaction of latex agglutination with an appropriate ligand being involved. The study resulted in designing a method of phagocytosis implementation that can be used to evaluate the content of certain receptors in cellular membranes and, subsequently, to define the changed receptor cell apparatus caused by a disease. The nature of polymer microspheric bioligands immobilized to the surface was shown to influence significantly the activity of leukocytic blood cells.
