ABSTRACT
The vast majority of recent efforts to synthesize effective antisense preparations for antiviral therapy have been made by using oligonucleotides, agents able to inhibit only a relatively small portion of the viral genome. That is the main reason why this approach is far from optimal. The optimal approach, however, is the accomplishment of the total inhibition of the viral genome. Here, a general protocol for preparation of the antisense antiviral agents with optimal properties for in vivo application is proposed, irrespective of the viral genome nucleotide sequence.
Subject(s)
Antiviral Agents/chemical synthesis , Oligonucleotides, Antisense/chemical synthesis , Antiviral Agents/pharmacology , DNA, Complementary , Genome, Viral , Oligonucleotides, Antisense/pharmacology , Reverse Transcriptase Polymerase Chain ReactionABSTRACT
An analysis of anticancer agents' selection based on the effect on tumor cell colony formation inhibition after exposure to the agent under investigation is performed. The real significance of such a method, as well as the probable negative clinical consequences of its use for screening of agents for cancer therapy are pointed out. Also, principles of overcoming the inadequacy of tumor cell clones' expansion inhibition tests and their equivalents are proposed.
