Merging molecular catalysts and metal-organic frameworks for photocatalytic fuel production.

In the effort to generate sustainable clean energy from abundant resources such as water and carbon dioxide, solar fuel production-the combination of solar-light harvesting and the generation of efficient chemical energy carriers-by artificial molecular photosystems is very attractive. Molecular constituents that display attractive features for chemical energy conversion (such as high product selectivity and atom economy) have been developed, and their interfacing with host materials has enabled recyclability, controlled site positioning, as well as access to fundamental insights into the catalytic mechanism and environment-governed selectivity. Among the wide variety of supports, metal-organic frameworks (MOFs) possess valuable characteristics (such as their porosity and versatility) that can influence the reaction environment and material architecture in a unique fashion. Here we highlight the various existing synthetic strategies to graft molecular complexes such as catalysts and photosensitizers onto MOFs for solar fuel production. The opportunities and limitations of one-pot and stepwise approaches are critically assessed, and the resulting materials are discussed based on their photocatalytic performances and the practical applicability of selected examples.

Efficacy of peroxygen compounds against glutaraldehyde-resistant mycobacteria.

BACKGROUND: To prevent cross-infection of patients, semicritical devices must be high-level disinfected by a product capable of destroying mycobacteria. Glutaraldehyde is commonly used; however, recent studies showed that glutaraldehyde-resistant mycobacteria could survive treatment with this chemical for extended exposure times. Our study tested hydrogen peroxide, peracetic acid, and a germicide (Cidex PA, Advanced Sterilization Products, Irvine, Calif) containing a mixture of the 2 peroxygen compounds for their ability to kill both glutaraldehyde-resistant and nonresistant (control) mycobacteria. METHODS: Bacterial suspensions were exposed to the test chemicals for various periods followed by neutralization and enumeration of survivors. RESULTS: Hydrogen peroxide at 10% and acidified hydrogen peroxide at 6% had low activity (<4 log reduction in 60 minutes exposure) against glutaraldehyde-resistant strains and slightly higher activity (4 log to 6 log reduction in 60 minutes) against the control strains. Peracetic acid at 0.07% had low to moderate activity (0.6 log to 6 log reduction in 60 minutes) against the resistant organisms and moderate to high activity (4 log to 6 log reduction in 10 minutes) against the control strains. Cidex PA, which contains a mixture of 0.07% peracetic acid and 1% hydrogen peroxide, had high activity (6 log reduction in 10 minutes) against all organisms. Efficacy of 0.8% phenol, a standard reference solution, did not correlate with efficacy of the peroxygen compounds. CONCLUSIONS: Hydrogen peroxide and peracetic acid had much higher activity toward mycobacteria when combined as a synergistic mixture than when evaluated individually.

Factors affecting the irreversible attachment of Pseudomonas aeruginosa to stainless steel.

To better understand the interaction between bacteria and surfaces, we studied the irreversible attachment of Pseudomonas aeruginosa to a common surfacing material. When brought into contact with the steel, cells began to attach in less than 1 min and the number adhering increased with time. An important physiological variable in attachment was cell motility since adherence decreased at least 90% when flagella were removed by blending. This treatment was shown to be effective because it caused motility loss and not because it removed a structure necessary for adherence. Cell viability was less important since adherence decreased only 50% when the number of viable cells was reduced 4.7 logs by heating or formaldehyde treatment. Significant environmental variables included turbulence and ionic strength. Attachment of motile cells was reduced 90% by agitation, although agitation had little effect on adherence of nonmotile cells. Both motile and nonmotile cells adhered poorly in distilled water with attachment increasing as CaCl2 or NaCl concentration increased to 10 mM. At 100 mM, attachment decreased. Viable cells, both motile and nonmotile, adhered best at a pH of 7 to 8, whereas nonviable cells attached most rapidly at a low pH.

Serological Diversity of Nitrobacter spp. from Soil and Aquatic Habitats.

Serotypic diversity among Nitrobacter spp. isolates is greater than previously reported. Typing with fluorescent antibodies prepared against 11 Nitrobacter spp. cultures isolated from soil and water placed the isolates into six serogroups. When these fluorescent antibodies were applied to a group of 16 additional isolates, 8 were identifiable by cross-reaction to 3 of the 11 fluorescent antibodies. Some nitrite-oxidizing enrichment cultures from different habitats contained cross-reacting strains of Nitrobacter spp.

High numbers of prosthecate bacteria in pulp mill waste aeration lagoons.

Prosthecate bacteria comprised 0.6 to 10.5% of the bacterial community in samples from 11 pulp mill waste aeration lagoons. Because of their distinct morphology, the genera Ancalomicrobium, Caulobacter, Prosthecobacter, Prosthecomicrobium, Stella, and Hyphomicrobium or Hyphomonas could be identified and enumerated by direct microscopic examination. Monthly samples from one lagoon showed that several genera varied from undetectable to predominant among the appendaged organisms. Temperature (season), type of wood pulped, and pulping process did not significantly affect the density of prosthecate bacteria.