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1.
Article in Russian | MEDLINE | ID: mdl-11569280

ABSTRACT

The population and subpopulation composition of peripheral blood lymphocytes of 33 women was studied in the dynamics of non-complicated pregnancy by the method of flow cytofluorometry with the use of equipment and reagents supplied by Becton Dickinson, Inc. This study revealed that the non-complicated course of pregnancy was characterized by the adequate reaction of the mother's immune system ensuring the physiological development of the fetus during pregnancy and timely labor.


Subject(s)
Lymphocytes/cytology , Pregnancy/immunology , Adult , Antigens, CD19/analysis , CD3 Complex/analysis , CD4-Positive T-Lymphocytes/cytology , CD56 Antigen/analysis , CD8-Positive T-Lymphocytes/cytology , Female , Flow Cytometry , Humans , Lymphocyte Count , Lymphocyte Subsets/cytology , Receptors, IgG/analysis
2.
Article in Russian | MEDLINE | ID: mdl-3521160

ABSTRACT

To control the effectiveness of vaccination against influenza, the optimum conditions for making the enzyme-linked immunosorbent assay (ELISA) with a view to determine the level of anti-influenza antibodies in human blood sera have been established. The kinetics of influenza virus adsorption in the wells of ELISA polystyrene plates and the kinetics of the interaction between the immobilized antigen and species-specific peroxidase-labeled antibodies have been studied. The method has been shown to be more sensitive than the hemagglutination inhibition test in the determination of seroconversion in persons immunized with influenza vaccine.


Subject(s)
Antibodies, Viral/analysis , Influenza Vaccines/immunology , Influenza, Human/immunology , Vaccination , Adsorption , Antibody Specificity , Antigens, Viral/immunology , Humans , Immunoenzyme Techniques , Influenza A virus/immunology , Quality Control , Time Factors
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