Changes in the Circulatory and Lymphatic Systems of Internal Genitals in Female Rats after Intravenous and Lymphotropic Administration of Multipotent Mesenchymal Stem Cells and Products Secreted by These Cells.

We studied the effects of intravenous and lymphotropic administration of bone marrow multipotent mesenchymal stromal cells and products secreted by these cells into conditioned medium on the blood and lymph circulation in the uterus and ovaries, as well as on folliculogenesis in female Wistar rats. It was shown that stromal cells and conditioned media of these cells administered via both routes lead to an increase in the number and diameter of blood vessels in the uterine wall and in the cortical layer of the ovaries. Neither mesenchymal stromal cells, not conditioned media affected the ovarian follicular apparatus.

Effects of Bone Marrow Multipotent Mesenchymal Stromal Cells and Their Secretory Products on Microcirculation in the Broad Ligament of the Uterus of Wistar Rats during Experimental Chronic Genital Inflammation.

Effects of bone marrow multipotent mesenchymal stromal cells and their secretory products released into the conditioned medium on microcirculatory bed in the broad ligament of the uterus were studied in Wistar rats with chronic genital inflammation. Opposite changes in the parameters of microcirculation and lymphatic drainage in the broad ligament of the uterus were observed after administration of cells and conditioned medium via different routes, which should be taken into account during the treatment of inflammatory and degenerative processes in the pelvic organs.

Cell Composition of Central and Peripheral Lymphoid Organs of Wistar Rats Treated with a Biomedical Cell Product.

We studied the effect of a biomedical cell product (bone marrow multipotent mesenchymal stromal cells and products secreted by these cells in conditioned medium) on subpopulation composition of lymphoid cells of central and peripheral lymphoid organs of Wistar rats under normal conditions. Changes in the subpopulation composition of lymphoid organs depended on the route of administration of biomedical cell product.

Content of bioelements in the lungs and liver in rats with alimentary obesity.

The synchrotron radiation X-ray fluorescence technique (SRXRF) was applied to the determination of K, Ca, Mn, Fe, Cu, Zn, Se, Br, Rb, and Sr concentrations in the liver and lungs in Wistar rats. The animals in the experiment included (1) healthy rats, (2) rats with alimentary obesity (AO), and (3) rats with alimentary obesity that were being given zinc sulphate with water for a long time (АО+Zn). Each group was divided into two subgroups. The experiment with the first subgroup was terminated with the animals in the state of physiological hunger and subsequent retrieval of liver and lung tissue, while the animals of the second subgroup were sacrificed two hours after ingestion of lard. The rats in physiological hunger manifested intergroup differences in the content of the bioelements (BEs) neither in the liver nor in the lungs. The rats with AO, as compared with the healthy animals, demonstrated in physiological hunger redistribution of inter-element correlations (IECs), which is an indirect reflection of sustained metabolic disorder. Additional zinc in the rats' ration did not affect their body weight and the concentration of the BEs (including zinc) in the liver and the lungs. However, the IECs in the tissues of these animals in physiological hunger also changed. This redistribution differed from that in the rats with AO. The IECs soon after ingestion of lard also changed, which also reflects sustained changes in the metabolism in the animals.

Parameters of Microcirculation in the Broad Ligament of the Uterus in Wistar Rats after Injection of Autologous Biomedical Cell Product.

We studied the effects of autologous biomedical cell product (bone marrow multipotent mesenchymal stromal cells and their conditioned media) on the parameters of the microcirculatory bed in the broad ligament of the uterus of normal Wistar rats were studied. The parameters of microcirculation and lymph drainage in the broad ligament changed in opposite directions in response to injection of autologous biomedical cell product via different routes. This fact should be taken into consideration when prescribing cell therapy for inflammatory degenerative processes in the pelvic organs.

[Interelement correlations in the lungs and the liver in alimentary obesity].

Using a high-fat diet in experiment on rats received model alimentary obesity (AO). Adding zinc to the diet did not affect the severity of the AO. The concentration of K, Ca, Mn, Fe, Cu, Zn, Se, Br, Rb, Sr in the liver and lungs of rats with obesity in both groups did not differ from that of healthy animals. As in the liver and lungs in rats of both groups with obesity has been a redistribution of interelement correlation (COP) and during physiological hunger and soon after consumption of pork fat. Redistribution of interelement COP in a state of physiological hunger reflects the strong changes of metabolic processes of the body. The nature of rearrangements of the COP in the liver and lungs were significantly different in each group of rats. Soon after intake of fatty foods in control rats and rats AO not receiving zinc, found an increase in the total number interelement COP in the lungs and major decline in the liver, which indirectly indicates the active participation of the lungs in the metabolism of dietary fat, over-expressed in control animals. In rats receiving more zinc, the increase in the total number of correlations after eating found in both bodies.

[Essential elements (Fe, Cu, Mn, Zn, Se, As) in the liver and lungs with alimentary obesity in the experiment].

The purpose - to establish the role of lungs in the metabolic processes in normal and alimentary obesity. The method of X-ray fluorescence analysis using synchrotron radiation, the determination of concentrations Fe, Cu, Mn, Zn, Se, As in the tissue of the lungs and liver in 19 control rats and 19 rats with alimentary obesity. On the basis of many strengths and very strong positive correlation between the content of chemical elements shows that in the control rats, fasting metabolism in the lung more intense than the liver, which in case of obesity is significantly reduced. After receiving the lard in the lungs decreases the number of correlations between the concentrations of microelements in the control animals. Obesity changing their character, indicating a switch of metabolism in connection with the utilization of triglycerides in the body of the control rats, and a malfunction of this process in obesity.

[From the history of the Kirov Evacuation Hospital N 1322 during the war].

During the Great Patriotic War in Kirov Red Army soldiers provided specialized psychiatric care. 24 June 1942 on the basis of evacohospital N 1322 was organized by the psychiatric ward. This department carries out all necessary psychiatric care until November 1945.

[Role of the lungs in utilization of triglycerides from blood plasma after administration of fat in norm and experimental obesity].

The experiment was carried out on 72 rats (36 control and 36 with alimentary adiposity). There were established the absence of distinctions in concentration of triglyceride in plasma of blood from the right auricle and a carotid at rats with adiposity in 2 hours after the unitary use of lard in comparison with control rats. Plasma of blood from the right auricle of control animals contained more triglyceride and this facts indirectly testifies to decrease of their recycling in lungs at adiposity.

[The state of the lymphatic system in inflammation of the internal sexual organs]. / Sostoianie limfaticheskoi sistemy pri vospalenii vnutrennikh polovykh organov.

In conditions of experimental inflammation of internal sexual organs in female rats fast and adequate reorganization of its lymphatic bed was found. In the nidus of inflammation sufficient drainage of tissues was retained. Loose and widened sinuses of ileal lymph nodes provided sufficient intranodal lymphodynamics. Formation of additional barrier composed of lymphoid cells was detected in paravaginal fat. Reduction of the speed of lymph flow within lymphangions promoted adequate conveyance of toxic lymph to nodes and its further processing. Mobilization of all links of lymphatic barrier resulted in its maximum activation and self recovery by d 14.

[Morphological anomalies in "tailed" lymphocyte nuclei and their connection with dicentric chromosomes in irradiated patients]. / Morfologicheskie anomalii iader tipa "khvostov" v limfotsitakh i ikh sviaz' s ditsentricheskimi khormosomami u obluchennykh patsientov.

Lymphocyte nuclei with a narrow outgrowth into the cytoplasm ("tail") were detected on peripheral blood smears of patients exposed to ionizing radiation during the clean up after the Chernobyl meltdown (liquidators). On average, lymphocytes with tailed nuclei occurred at a frequency of 0.43% and 0.15% in exposed patients (n = 101) and healthy donors (n = 50), respectively; the difference between the samples was significant, P < 0.001. The coefficient of correlation between frequencies of lymphocytes with tailed nuclei and those with dicentric chromosomes was 0.74 (n = 27, P < 0.001). The frequency of lymphocytes with dicentric chromosomes was increased in 9 out of 10 patients, in which lymphocytes with tailed nuclei occurred at a frequency larger than 0.8%. A double-color FISH was used to localize centromeric (not more than two signals) and telomeric (not more than one signal) regions in nuclear tails. Abnormal tailed lymphocyte nuclei were assumed to result from breaking the chromosome bridges formed by the dicentric chromosomes.

[The use of lyophilized plasma as a reference sample for determining factor VIII]. / Ispol'zovanie liofilizirovannoi plazmy kak referentnogo obraztsa dlia opredeleniia faktora VIII.

The activity of lyophilized Soviet reference plasma Factor VIII was measured by a single-stage Papayan and Khrolova's method. This factor is referent in respect of the prothrombin complex factors, stored for 18-43.5 months. Factor VIII activity was found to be within 55-135 percent. Differences in the values of antihemophilic globulin activities obtained in parallel studies in different days may be explained by the fact that the native control was not standard. The results are promising as regards the possibility of using lyophilized donor reference plasma as a reference reagent for estimation of Factor VIII activity.

[A lyophilized reagent for standardization of the activated partial thromboplastin time]. / Liofilizirovannyi reagent dlia standartizatsii aktivirovannogo partsial'nogo tromboplastinovogo vremeni.

Native partial thromboplastin, prepared from cadaver brain thromboplastin (a reagent), is a cephalin analog used to detect disorders in the first phase of coagulation. It was lyophilized with the aim of its stabilization, potential commercial manufacture, and introduction into practical clinical laboratories. Native emulsion was distributed into 0.2 ml penicillin flasks and lyophilized for 18-20 hrs in IZ-9 (CSR) or similar equipment at the maximum temperature of the product 5 degrees C. Lyophilized reagent is a white or slightly cream-colored powder. It retains its activity for at least a year as evidenced by the activated partial thromboplastin time test. The range of activities within one lot is less if commercial lyophilized donor reference plasma is used in the test. The reagent is stored in a refrigerator at 4-8 degrees C.

[Evaluation of single-stage methods of studying the activity of factor VIII]. / Otsenka odnostadiinykh metodov issledovaniia aktivnosti faktora VIII.

Accurate diagnosis of coagulopathies related to changed factor VIII activity, studies of specific activities of antihemophilic cryoprecipitate globulin used for hemostatic correction in patients, and monitoring of therapy efficacy are impossible without a reproducible, sensitive, standard, and available method. The authors have compared two single-stage methods developed by Soviet scientists: that by V. P. Baluda et al. and that by L. P. Papayan and P. V. Khrolova. Both methods have proved sufficiently sensitive and capable to detect factor VIII deep deficiency, but L. P. Papayan and P. V. Khrolova's modification was found higher reproducible, and therefore chosen for further investigation in order to standardize it. Besides the conditions of reproducing the method, reference plasma, substrate plasma deficient for factor VIII, and partial thromboplastin are to be standardized.