Screening and GC-MS profiling of ethanolic extract of Tylophora pauciflora.

Medicinal plants are boundless source of raw materials for the pharmaceutical. Identification of natural compounds from medicinal plant is helpful in the discovery of novel therapeutic agents. Tylophora pauciflora is a medicinal plant, which possess many biological activities such as antioxidant activity, anti-inflammatory activity and anti cancer activity. There is no GC-MS analysis reported on this plant. Thus, the present study is aimed to identify the present of phyto-chemical compounds from ethanolic extract of Tylophora pauciflora using GC-MS analysis. Results, the extract possess totally 14 bioactive compounds among that natural compound of n-hexadecanoic acid has highest % peak area and it have the variety of biological activities such as; anti-oxidant, 5-alpha-reductase-inhibitor, anti-fibrinolytic, hemolytic, antimicrobial activity, hypo-cholesterolemic, nematicide, pesticide, anti-androgenic flavor and hemolytic. It is concluded that the ethanolic extract of Tylophora pauciflora have biologically active compounds. In future by isolating and identifying, these compounds can be considered to treat the human disorders.

Evaluation of in vitro enzymatic and non-enzymatic antioxidant properites of leaf extract from Alpinia Purpurata (Vieill.) K. Schum.

OBJECTIVE: To evaluate the enzymatic and non-enzymatic antioxidants of leaf extract from Alpinia purpurata. METHODS: One gram of fresh leaf of Alpinia purpurata was grinded in 2 mL of 50% ethanol and centrifuged at 10,000×g at 4°C for 10 min. The supernatant obtained was used within 4 h for various enzymatic antioxidants assays like superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione S-transferase (GST), ascorbate oxidase, peroxidase, polyphenol oxidase (PPO) and non-enzymatic antioxidants such as vitamin C, total reduced glutathione (TRG) and lipid peroxidation (LPO). RESULTS: The leaf extract of Alpinia purpurata possess antioxidants like vitamin C 472.92±6.80 µg/mg protein, GST 372.11±5.70 µmol of 1-chloro 2,4 dinitrobenzene (CDNB)-reduced glutathione (GSH) conjugate formed/min/mg protein, GPx 281.69±6.43 µg of glutathione oxidized/min/mg protein, peroxidases 173.12±9.40 µmol/g tissue, TRG 75.27±3.55 µg/mg protein, SOD 58.03±2.11 U/mg protein, CAT 46.70±2.35 µmol of H2O2 consumed/min/mg protein in high amount whereas ascorbate oxidase 17.41±2.46 U/g tissue, LPO 2.71±0.14 nmol/L of malondialdehyde formed/min/mg protein and PPO 1.14±0.11 µmol/g tissue in moderate amount. CONCLUSION: Alpinia purpurata has the potential to scavenge the free radicals and protect against oxidative stress causing diseases. In future, Alpinia purpurata may serve as a good pharmacotherapeutic agent.

Isolation, structural characterization and in silico drug-like properties prediction of a natural compound from the ethanolic extract of Cayratia trifolia (L.).

BACKGROUND: Natural products have continually played an important role in drug discovery because it serves as active principles in drugs as well as templates for synthesis of new drugs. Cayratia trifolia (L.) is a medicinal plant, which has been reported to have antiviral, antibacterial, antiprotozoal, hypoglycemic, anticancer and diuretic activities. OBJECTIVE: Therefore, the objective of this study is to isolate and identify the natural compound from the ethanolic extract of Cayratia trifolia (L.) and to predict the Absorption, Distribution, Metabolism and Excretion (ADME) properties of isolated natural compound. MATERIALS AND METHODS: Column chromatography and thin layer chromatography were used to isolate the natural compound and Fourier-transform infrared (FTIR) spectroscopy was used to predict the functional groups present in the isolated natural compound. The structural characterization studies were functionally carried out using (1)H, (13)C, two-dimensional nuclear magnetic resonance (NMR) and mass spectrometry methods. RESULTS: FTIR showed that, the groups of OH, C-H, C = C may be present in the isolated natural compound. (1)H, (13)C, two-dimensional NMR and mass spectrometry data suggests that the isolated natural compound probably like linoleic acid. In silico ADME properties, prediction of the compound was under acceptable range. CONCLUSION: Based on the results, it can be concluded that, the isolated natural compound of linoleic acid that has been exhibited good medicinal properties.

Leaf extract of Alpinia purpurata (Vieill.) K. Schum screened for its phytochemical constituents and antibacterial and anticancer activities.

OBJECTIVE: The study was formulated with the objective to assess phytochemical constituents, antibacterial activity and anticancer activity of Alpinia purpurata. METHODS: The leaves of A. purpurata were washed thoroughly by tap water, shade dried and powdered. The plant powder was extracted with successive solvent system. Phytochemical constituents were evaluated, antibacterial activity was carried out by disc diffusion method and anticancer activity of the ethyl acetate leaf extract was evaluated by using 3-(4,5 dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. RESULTS: The ethyl acetate extract of A. purpurata showed most of the phytochemicals. The extract exhibited antibacterial activity with a zone of inhibition from 5 to 14 mm at various concentrations and the extract showed potential anticancer activity against PA1 ovarian cancer cell line at the 48 h with half maximal inhibitory concentration value of 110.25 µg/mL and exhibited a dose-dependent decrease in cell count for all the concentrations tested. CONCLUSION: The present study scientifically proved that ethyl acetate leaf extract of A. purpurata is a good source of phytoconstituents, showing antibacterial and anticancer activities.

Leaf extract of Alpinia purpurata (Vieill.) K. Schum screened for its phytochemical constituents and antibacterial and anticancer activities / 中西医结合学报

The study was formulated with the objective to assess phytochemical constituents, antibacterial activity and anticancer activity of Alpinia purpurata.