1.
Ukr Biokhim Zh (1999)
; 82(6): 14-21, 2010.
Article
in Ukrainian
| MEDLINE
| ID: mdl-21805858
ABSTRACT
Purified human arginase I preparations homogeneous in SDS-PAAG test were obtained by the affinity chromatography on the synthesized sorbent L-arginine-macroporous glass. Some physico-chemical characteristics of the isolated arginase preparation have been estimated: thermo- and pH-stability, temperature- and pH-optima of the enzyme. The influence of some bivalent metal ions and other additives on enzymatic activity for stabilization of the enzyme and optimization of its storage conditions was studied.