1.
Chem Commun (Camb)
; 56(25): 3685-3688, 2020 Mar 26.
Article
in English
| MEDLINE
| ID: mdl-32119023
ABSTRACT
Fragment antigen-binding domains (Fabs) from anti-Frizzled and anti-LRP6 monoclonal antibodies were conjugated using SpyTag-SpyCatcher chemistry via a one-pot reaction. The resulting synthetic heterodimeric agonist outperformed the natural ligand, Wnt-3a, in activating canonical Wnt signaling in mammalian cells. This approach should be broadly applicable to activate receptor-mediated cellular signaling.