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1.
Biochemistry ; 44(19): 7369-77, 2005 May 17.
Article in English | MEDLINE | ID: mdl-15882076

ABSTRACT

Tryptophan residues may play several roles in integral membrane proteins including direct interaction with substrates. In this work we studied the contribution of tryptophan residues to substrate binding in EmrE, a small multidrug transporter of Escherichia coli that extrudes various positively charged drugs across the plasma membrane in exchange with protons. Each of the four tryptophan residues was replaced by site-directed mutagenesis. The only single substitutions that affected the protein's activity were those in position 63. While cysteine and tyrosine replacements yielded a completely inactive protein, the replacement of Trp63 with phenylalanine brought about a protein that, although it could not confer any resistance against the toxicants tested, could bind substrate with an affinity 2 orders of magnitude lower than that of the wild-type protein. Double or multiple cysteine replacements at the other positions generate proteins that are inactive in vivo but regain their activity upon solubilization and reconstitution. The findings suggest a possible role of the tryptophan residues in folding and/or insertion. Substrate binding to the wild-type protein and to a mutant with a single tryptophan residue in position 63 induced a very substantial fluorescence quenching that is not observed in inactive mutants or chemically modified protein. The reaction is dependent on the concentration of the substrate and saturates at a concentration of 2.57 microM with the protein concentration of 5 microM supporting the contention that the functional unit is a dimer. These findings strongly suggest the existence of an interaction between Trp63 and substrate, and the nature of this interaction can now be studied in more detail with the tools developed in this work.


Subject(s)
Antiporters/chemistry , Antiporters/metabolism , Drug Resistance, Multiple, Bacterial , Escherichia coli Proteins/chemistry , Escherichia coli Proteins/metabolism , Membrane Proteins/chemistry , Membrane Proteins/metabolism , Tryptophan/chemistry , Amino Acid Sequence , Amino Acid Substitution/genetics , Antiporters/genetics , Biological Transport/genetics , Drug Resistance, Multiple, Bacterial/genetics , Escherichia coli Proteins/genetics , Membrane Proteins/genetics , Molecular Sequence Data , Mutagenesis, Site-Directed , Onium Compounds/metabolism , Organophosphorus Compounds/metabolism , Phenotype , Phenylalanine/genetics , Protein Binding/genetics , Protein Conformation , Spectrometry, Fluorescence/methods , Tryptophan/genetics , Tyrosine/genetics
2.
Biochemistry ; 43(23): 7491-502, 2004 Jun 15.
Article in English | MEDLINE | ID: mdl-15182191

ABSTRACT

The involvement of transporters in multidrug resistance of bacteria is an increasingly challenging problem, and most of the pumps identified so far use the protonmotive gradient as the energy source. A new member of the ATP-binding cassette (ABC) family, known in Bacillus subtilis as YvcC and homologous to each half of mammalian P-glycoprotein and to LmrA of Lactococcus lactis, has been studied here. The yvcC gene was constitutively expressed in B. subtilis throughout its growth, and a knockout mutant showed a lower rate of ethidium efflux than the wild-type strain. Overexpression of yvcC in Escherichia coli allowed the preparation of highly enriched inverted-membrane vesicles that exhibited high transport activities of three fluorescent drugs, namely, Hoechst 33342, doxorubicin, and 7-aminoactinomycin D. After solubilization with n-dodecyl beta-D-maltoside, the hexahistidine-tagged YvcC was purified by a one-step affinity chromatography, and its ability to bind many P-glycoprotein effectors was evidenced by fluorescence spectroscopy experiments. Collectively, these results showed that YvcC is a multidrug ABC transporter functionally active in wild-type B. subtilis, and YvcC was therefore renamed BmrA for Bacillus multidrug resistance ATP. Besides, reconstitution of YvcC into liposomes led to the highest, vanadate-sensitive, ATPase activity reported so far for an ABC transporter. Interestingly, such a high ATP hydrolysis proceeds with a positive cooperativity mechanism, a property only found so far with ABC importers.


Subject(s)
ATP-Binding Cassette Transporters/genetics , ATP-Binding Cassette Transporters/metabolism , Bacillus subtilis/metabolism , Bacterial Proteins/metabolism , Dactinomycin/analogs & derivatives , Membrane Transport Proteins/metabolism , ATP-Binding Cassette Transporters/chemistry , ATP-Binding Cassette Transporters/isolation & purification , Adenosine Triphosphate/metabolism , Amino Acid Sequence , Bacillus subtilis/drug effects , Bacillus subtilis/genetics , Bacterial Proteins/chemistry , Bacterial Proteins/genetics , Bacterial Proteins/isolation & purification , Benzimidazoles/metabolism , Biological Transport/drug effects , Cell Membrane/drug effects , Dactinomycin/metabolism , Doxorubicin/metabolism , Drug Resistance, Bacterial , Escherichia coli/drug effects , Ethidium/metabolism , Liposomes/chemistry , Liposomes/metabolism , Membrane Transport Proteins/chemistry , Membrane Transport Proteins/genetics , Membrane Transport Proteins/isolation & purification , Molecular Sequence Data , RNA, Messenger/genetics , RNA, Messenger/metabolism , Reserpine/pharmacology , Sequence Alignment , Vanadates/pharmacology
3.
Biochim Biophys Acta ; 1565(1): 1-5, 2002 Sep 20.
Article in English | MEDLINE | ID: mdl-12225846

ABSTRACT

ATP-binding cassette (ABC) transporters have often been refractory to over-expression. Using the C41(DE3) E. coli as a host strain, membrane vesicles highly enriched (>50%) in YvcC, a previously uncharacterized ABC transporter from Bacillus subtilis homologous to P-glycoprotein multidrug transporters, were obtained. The functionality of YvcC was assessed by its high vanadate-sensitive ATPase activity and its ability to transport a fluorescent drug, the Hoechst 33342.


Subject(s)
ATP-Binding Cassette Transporters/biosynthesis , Bacillus subtilis/genetics , Bacterial Outer Membrane Proteins/biosynthesis , Escherichia coli/metabolism , ATP Binding Cassette Transporter, Subfamily B, Member 1/biosynthesis , Benzimidazoles/metabolism , Escherichia coli/genetics , Fluorescent Dyes/metabolism , Vanadates
4.
J Mol Biol ; 315(5): 1075-85, 2002 Feb 01.
Article in English | MEDLINE | ID: mdl-11827477

ABSTRACT

YvcC, a multidrug transporter from Bacillus subtilis, is a member of the ATP-binding cassette superfamily, highly homologous to each half of human multidrug-resistance P-glycoprotein and to several other bacterial half-ABC transporters. Here, the purified recombinant histidine-tagged YvcC has been reconstituted into a lipid bilayer. Controlled and partial detergent removal from YvcC-lipid micelles allowed the production of particularly interesting lipid-detergent-YvcC ring-shaped particles, about 40 nm in diameter, well suited for single particle analysis by cryo-electron microscopy. Furthermore, binding of these histidine-tagged ring-shaped particles to lipid layers functionalized with a Ni(2+)-chelating head group generated a preferential perpendicular orientation, eliminating the missing cone in the final three-dimensional reconstruction. From such analysis, a computed volume has been determined to 2.5 nm resolution giving a detailed insight into the structural organization of this half-ABC transporter within a membrane. The repetitive unit in the ring-shaped particles is consistent with a homodimeric organization of YvcC. Each subunit was composed of three domains: a 5 nm height transmembrane region, a stalk of about 4 nm in height and 2 nm in diameter, and a cytoplasmic lobe of about 5-6 nm in diameter. The latest domain, which fitted with the reported X-ray structure of HisP, was identified as the nucleotide-binding domain (NBD). The 3D reconstruction of the YvcC homodimer well compared with the very recent X-ray crystallographic data on the MsbA homodimer from Escherichia coli, supporting the existence of a central open chamber between the two subunits constituting the homodimer. In addition, the 3D reconstruction of YvcC embedded in a membrane revealed an asymmetric organization of the two NBDs sites within the homodimer, as well as a dimeric interaction between two homodimers.


Subject(s)
ATP-Binding Cassette Transporters/chemistry , ATP-Binding Cassette Transporters/ultrastructure , Bacillus subtilis/chemistry , Bacterial Proteins/chemistry , Bacterial Proteins/ultrastructure , Cryoelectron Microscopy , ATP-Binding Cassette Transporters/genetics , ATP-Binding Cassette Transporters/metabolism , Amino Acid Transport Systems, Basic/chemistry , Bacillus subtilis/genetics , Bacterial Proteins/genetics , Bacterial Proteins/metabolism , Crystallography, X-Ray , Detergents/metabolism , Dimerization , Escherichia coli Proteins/chemistry , Lipid Bilayers/metabolism , Lipid Metabolism , Membrane Proteins/chemistry , Micelles , Models, Molecular , Nickel/metabolism , Protein Binding , Protein Structure, Quaternary , Protein Structure, Tertiary , Protein Subunits , Recombinant Fusion Proteins/chemistry , Recombinant Fusion Proteins/metabolism , Recombinant Fusion Proteins/ultrastructure
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