ABSTRACT
In this paper, we describe an electrochemical sensing platform-ElectroSens-for the detection of Zn based on self-assembled polyelectrolyte multilayers on the carbon fiber (CF) electrode surface. The CF-extended surface facilitates the usage of a small volume electrochemical cell (1 mL) without stirring. This approach allows making a low-cost three-electrode platform. Working electrode modification with layer-by-layer assembly of polyethyleneimine (PEI), poly(sodium 4-styrenesulfonate) (PSS), and mercury nitrate layers eliminates solution toxicity and provides stable stripping voltammetry measurements. The stable, robust, sustainable, and even reusable Ag/AgCl reference electrode consists of adsorbed 32 PEI-KCl/PSS-KCl bilayers on the CF/silver paste separated from the outer solution by a polyvinyl chloride membrane. The polyelectrolyte-based sensor interface prevents adsorption of protein molecules from biological liquids on the CF surface that leads to a sensitivity increase of up to 2.2 µA/M for Zn2+ detection and provides a low limit of detection of 4.6 × 10-8 M. The linear range for Zn detection is 1 × 10-7 to 1 × 10-5 M. A portable potentiostat connected via wireless to a smartphone with an android-based software is also provided. The ElectroSens demonstrates reproducibility and repeatability of data for the detection of Zn in blood and urine without the digestion step.
ABSTRACT
This work depicts an electrochemical hydrogel-eutectic gallium indium alloy interface for the detection of tick-borne encephalitis (TBE) virus. This interface allows recording of nonlinear current-voltage responses, depending on the composition of the hydrogel. The current-voltage data for the machine learning model are trained by a multilayer perceptron. This model accurately recognizes the TBE antibody, antigen, and an antibody-antigen complex in mixture with interfering bovine serum albumin with 93% accuracy. Thus, this interface can be used as a convenient method for expressed viruses and pathogens detection.
ABSTRACT
A novel flexible ion-selective sensor for potassium and sodium detection was proposed. Flexible ion-selective electrodes with pseudo-liquid internal solution on contrary to the system with a solid contact provided a more stable analytical signal. Such advantages were achieved because of polyelectrolyte (PEI/PSS) layers adsorption on the conduct substrate with a layer-by-layer technique. Such an approach demonstrated that ion-selective electrodes save sensitivity with Nernstian dependence: 56.2 ± 1.4 mV/dec a Na+ and 56.3 ± 1.9 mV/dec a K+ , as well as a fast time of response for potassium (5 s) and sodium (8 s) was shown. The sensing platform proposed demonstrates a better time of response and is close to the Nernstian value of sensitivity with a sensor low cost. The results proposed confirm a pseudo-liquid junction for the ion-selective electrode. Biocompatibility of an ion-selective sensing platform was demonstrated at potassium potentiometric measurements in Escherichia coli biofilms. Potassium levels in a biofilm were measured with potentiometry and showed agreement with the previous results.
