Occurrence of trace elements in Mediterranean mussels (Mytilus galloprovincialis Lamarck, 1819) from an experimental pilot farm in the Calich Lagoon (Sardinia, Italy).

The present study aimed to determine trace elements in Mediterranean mussels (Mytilus galloprovincialis) from an experimental pilot farm of the Calich Lagoon, a typical Sardinian brackish area (Italy). Two sampling sessions were scheduled in February and May 2019 and the occurrence of 24 metals (Hg, Ag, Al, As, Be, Bi, Cd, Co, Cr, Cu, Fe, Ga, In, Mg, Mn, Mo, Ni, Pb, Rb, Se, Sn, Ti, V, Zn) in bivalves was considered. Environmental conditions of water (temperature, salinity, pH, dissolved oxygen, and chlorophyll a) were also measured in situ. A high significant (P<0.001) difference was reported for temperature, pH, and dissolved oxygen. Our results showed a significant sessional variation of Mo (P<0.001); Cd, V (P<0.01); Ni, Pb and Co (P<0.05) in examined M. galloprovincialis samples; as all values were higher in February than those for May session samples, meanwhile the highest levels were reported for Mg (mean±s.d. 1151±263 mg kg-1 wet weight), Al (mean±s.d. 341±192 mg kg-1 w.w.), and Fe (mean±s.d. 212 ±75 mg kg-1 w.w.) in February samples. The European Union uppermost values (EC Reg. 1881/2006) for Cd, Hg, and Pb were never overpassed. The results confirmed the role of M. galloprovincialis as one of the most appropriate biological indexes to track the presence of trace elements in brackish environments. It could be concluded that the current ecology of the Calich Lagoon suggests that compatibly with the transitional ecosystem, the classification as a bivalves' production area and the implementation of extensive shellfish farming can improve its production capacities. The knowledge of the lagoon ecology is an essential tool for its sustainable exploitation, preserving biodiversity, and mitigating the effects of anthropogenic activities on public health.

Seasonal accumulation of trace elements in native Mediterranean mussels (Mytilus galloprovincialis Lamarck, 1819) collected in the Calich Lagoon (Sardinia, Italy).

The occurrence of 21 trace elements in native Mediterranean mussel (Mytilus galloprovincialis) from the Calich Lagoon, a typical brackish area of the northwest of Sardinia (Italy), was investigated. The seasonal variation of metals in bivalves was considered, and the highest values were found in spring and summer; in particular, a high significant (P < 0.001) temporal variation was reported for silver (Ag) and mercury (Hg). The highest and similar concentrations were registered for aluminium (Al, mean 32 mg kg-1wet weight), iron (Fe, mean 32 mg kg-1 w. w.), and zinc (Zn, mean 25 mg kg-1 w. w.). The maximum limits set by European Regulations for cadmium (Cd), mercury (Hg), and lead (Pb) were never exceeded. Speciation analysis revealed negligible risk related to inorganic arsenic (iAs). Therefore, M. galloprovincialis confirmed its role as suitable bioindicator to monitor the contamination of coastal environments. Although the recommended tolerable weekly intake (TWI) was not exceeded, the levels of aluminium should be carefully evaluated in monitoring plans in the studied lagoon. Graphical abstract.

Distribution and bioaccumulation of trace elements and lanthanides in apples from Northwestern Italy.

BACKGROUND: Transfer of metals from soil to plant is a possible route of contamination for the food chain. This investigation focused on the occurrence of 40 elements in the "Red Apple of Cuneo", an Italian excellence and a Protected Geographical Indication (P.G.I.). Four cultivars were considered: Red Delicious (Jeromine) and Gala (Bukeye, Brookfield, Schniga). METHODS: Trace elements and rare earth elements (REEs) detection was performed by an Inductively Coupled Plasma-Mass Spectrometer (ICP-MS) previous homogenization of samples and microwave acid digestion. One-way analysis of variance (ANOVA) with Bonferroni correction was employed to for statistical analysis. RESULTS: Concentrations of metals in the four apples cultivars were quite comparable, Al and Rb were the most represented nonessential elements while Fe, Cu and Zn between the essential; REEs were found at negligible concentrations. Bioaccumulation factors demonstrated an extremely low capacity of bioaccumulation from soil to fruit. CONCLUSIONS: The analysis performed on the "Red Apple of Cuneo" has shown that this product is safe for human consumption since metals were recovered at concentrations of no concern and lower than those registered in apples from other countries. Since each production area is characterized by a typical elemental pattern the multielemental profile based on the analysis of 40 elements could be useful to relate products to their region of origin. Differences between the four apple cultivars were instead not significant to discriminate between them.

Trace and rare earth elements in monofloral and multifloral honeys from Northwestern Italy; A first attempt of characterization by a multi-elemental profile.

BACKGROUND: Metals are a minor constituent in honey, but they have been suggested to be a potential tool to characterize honeys according to their botanical or geographical origin. METHODS: A total of 40 metals were analyzed by Inductively Coupled Plasma-Mass Spectrometry in monofloral and multifloral honeys from Northwestern Italy. PCA and ANOVA were used to discriminate honeys according to the various floral types. RESULTS: The highest levels of trace elements and rare earth elements (REEs) were found in chestnut honey, while the lowest concentrations were recorded in acacia and rhododendron honeys. Rubidium and aluminum were the most represented nonessential elements, while manganese, iron, zinc and copper had the highest values of the essential elements. Potentially toxic elements arsenic, cadmium and lead were close to or below the limit of quantification. CONCLUSIONS: The present study showed significant differences in metal concentrations according to honey floral type, reflecting the strong influence of botanical origin on the chemical composition of this particular food.

Trace elements and rare earth elements in honeys from the Balkans, Kazakhstan, Italy, South America, and Tanzania.

Honey is a very unusual food - a natural substance produced by bees from the nectar or secretion of plants and elaborated by the bees themselves. Differences in trace elements and rare earth elements (REEs) are related to botanical and geographical origin, and, as such, honey could be a suitable indicator for metal occurrence in the environment within the bee forage area. A total of 40 metals were analyzed by inductively coupled plasma-mass spectrometry in multi-floral honey samples from different geographical areas. The highest levels of trace elements and REEs were found in honey from Tanzania, while lower concentrations were recorded in Italian, Kazakhstan, the Balkans, and South American honeys. Tanzanian honey is of interest due to its different content of metals, related to the peculiar lithology and the mineral resources of this country. Rubidium and aluminum were the most represented nonessential elements, while manganese, iron, zinc, and copper registered the highest values of the essential elements. Very low concentrations were found for the toxic elements lead, cadmium, and arsenic. The present study showed differences in metal concentrations in multi-floral honey from different countries, confirming the strong influence of the area of origin on the chemical composition of honeys.

Differential Bioaccumulation of Trace Elements and Rare Earth Elements in the Muscle, Kidneys, and Liver of the Invasive Indo-Pacific Lionfish (Pterois spp.) from Cuba.

The Indo-Pacific lionfish is a saltwater fish that inhabits the Red Sea waters and the Indian and Pacific oceans; it is an invasive species in the western Atlantic and was recently introduced into the local diet in the USA, Central and South America, and the Caribbean with the aim of controlling the invasion of this species. Due to its predatory nature, it tends to bioaccumulate metals and other contaminants via the marine food web and could thus constitute a suitable species for monitoring aquatic ecosystems. The presence and distribution of 23 trace elements and 16 rare earth elements (REEs) were investigated by inductively coupled plasma-mass spectrometry (ICP-MS) in the muscle, liver, and kidneys of lionfish from Cuba. Significant differences in metal concentrations were found in the different fish organs. The liver and kidneys registered the highest concentrations for most trace elements and for ΣREE, thus demonstrating that they are effective bioindicators of possible pollution on the environment in which fish live, and assuming great importance in the choice of early biomonitoring. Trace element concentrations in the muscle are instead of crucial interest for consumer safety. The limits set by EU regulations and Cuban guidelines for Cd and Pb in fish muscle were never exceeded, suggesting that lionfish from Cuba could therefore represent a good source of minerals and proteins for the local population. Graphical abstract.

Feathers of Humboldt penguin are suitable bioindicators of Rare Earth Elements.

Rare earth elements (REEs), also called lanthanides, are emerging contaminants worldwide, due to their unique physical and chemical characteristics that make them essential in a variety of industrial applications. However, there is still a gap in the knowledge of occurrence and accumulation of REEs in biota, and no investigations have yet been performed in penguin feathers, which have already been widely utilized as a non-invasive tool for the biomonitoring of trace elements. The concentrations of 16 REEs were investigated in a colony of Humboldt penguins (Spheniscus humboldti) housed at the Acquario di Cattolica (Italy). Multielement determination of REEs was performed by an Inductively Coupled Plasma-Mass Spectrometer after a microwave digestion of feathers. As this colony lives indoors in a controlled environment, it was the ideal choice for studying lanthanide occurrence in penguin feathers. Since there is a strict link between metal levels in feathers and the diet of penguins, their food (capelin) was also tested for REEs. Chondrite normalized values revealed the same pattern for REEs in feathers and fish, but REE concentrations were an order of magnitude higher in penguin feathers, demonstrating the suitability of this tissue as a bioindicator of REEs.

Rare earth elements in marine and terrestrial matrices of Northwestern Italy: Implications for food safety and human health.

Rare earth elements (REEs) are central in several critical technologies; their use is constantly increasing as is their release into the environment. For this reason, it is important to investigate REE concentrations in different matrices to evaluate human exposure and environmental risk of these emerging contaminants. REEs were measured by ICP-MS in matrices of terrestrial (plant feed, fruit, honey, wildlife livers) and marine origin (seaweeds, zooplankton, bivalves, fish) collected from Northwestern Italy. Highest REE concentrations were measured at low trophic levels, both in terrestrial and marine environments, such as plants (ΣREE 1.8 mg kg-1) and seaweed (ΣREE 12 mg kg-1), the major source of exposure and transfer of REEs to food webs. REE concentrations were several orders of magnitude lower in fruit, honey, and livers from terrestrial wildlife, suggesting a negligible risk of exposure by these matrices. Marine biota, such as bivalves (ΣREE 0.16 mg kg-1) and fish (ΣREE 0.21 mg kg-1) may constitute a pathway for human or animal dietary exposure. The study confirmed that REEs have low potential for biomagnification, but instead are subject to trophic dilution. However, given the numerous sources of dietary introduction of REEs, they should be monitored for a possible harmful cumulative effect. Owing to the scarcity of data regarding REEs worldwide, our results contribute to assessment of the occurrence of these emerging contaminants.

Trace elements, rare earth elements and inorganic arsenic in seaweeds from Giglio Island (Thyrrenian Sea) after the Costa Concordia shipwreck and removal.

The occurrence of trace elements, REE and iAs was investigated in macroalgae collected from Giglio Island (Grosseto, Italy), 3 years after the Costa Concordia shipwreck recovery operations. There was a high variability of metals and REE between species, even those belonging to the same phylum. Arsenic level was found within the range of the Tuscany marine environment; the inorganic fraction was from 9% to 31%. Al, Be, Pb and Zn levels in seaweeds from Giglio Island were found to be significantly higher than in macroalgae from other islands of the Tuscany archipelago. REE were double the values found in the Tuscan islands of Elba and Capraia. We suggest that concentrations of metals and REE were influenced by the 3-year-long naval operations carried out for the Costa Concordia shipwreck rescue, and that macroalgae could be suitable bioindicators of perturbations in metal concentrations in the marine environment. CAPSULE: Inorganic contaminants in seaweeds for Giglio Island.