ABSTRACT
Isolated human peripheral blood lymphocytes were exposed to 254 nm UV light and stimulated with PHA. Chromosome analysis revealed significant increases in sister chromatid exchanges (SCE's) and micronuclei, and also in gross chromosomal aberrations, but not in breaks and gaps on the chromatid level. By means of flow cytometry it was shown that UV exposure perturbed the cell cycle in that the S and G2/M fraction decreased when UV was given before stimulation with PHA. Labelling the cells with fluorescein-conjugated antibodies did not reveal any changes in the relative frequencies of lymphocyte subsets after exposure, but an overall diminished ability to bind antibody was observed.
Subject(s)
Antigens, Surface/radiation effects , Chromosome Aberrations , Lymphocytes/radiation effects , Antibodies, Monoclonal , Cell Cycle/radiation effects , Humans , In Vitro Techniques , Lymphocytes/cytology , Lymphocytes/immunology , Phytohemagglutinins/pharmacology , Sister Chromatid Exchange/radiation effects , Ultraviolet Rays/adverse effectsABSTRACT
Chromosome analysis was performed on five persons before and after urographic examination. Despite a small increase in chromosome aberration frequency after the examination, no statistically significant increase was demonstrated. Whole body lymphocytes were analyzed for chromosome aberrations after exposure to low dose radiation, contrast agents and radiation together with a contrast medium. A significant increase in aberration frequencies at the 2.5% level was observed in irradiated blood samples containing the iodized contrast agent metrizoate.
Subject(s)
Chromosomes/diagnostic imaging , Iodobenzoates/adverse effects , Metrizoic Acid/adverse effects , Adult , Chromosome Aberrations , Female , Humans , Lymphocytes/drug effects , Lymphocytes/radiation effects , Male , Metrizoic Acid/pharmacology , Middle Aged , UrographySubject(s)
Background Radiation , Chromosome Aberrations , Lymphocytes/radiation effects , Radiation, Ionizing , Water Supply , Adolescent , Adult , Age Factors , Aged , Environmental Exposure , Epidemiologic Methods , Female , Finland , Humans , Male , Middle Aged , Radon , Time FactorsABSTRACT
Thoracic duct drainage and re-infusion of the irradiated lymph was carried out as immunosuppressive treatment in 2 patients with progressive, therapy-resistant rheumatoid arthritis. In both patients, a marked clinical improvement was achieved even during the first days of treatment. A reduced number of T cells in the blood was seen 3 days after onset of drainage, whereas no significant change in the number of B cells was observed. No recirculation of the infused cells could be detected, nor was the radiation removal of T cells accompanied by rapid proliferation of "new" T cells. As clinical improvement and reduction in T cells occurred simultaneously, there is probably a connection between these two events. The beneficial clinical response and the achievement of T cell suppression by thoracic duct drainage--the result of irradiation and re-infusion of irradiated lymph--encourage further clinical trials with this type of treatment in severe therapy-resistant rheumatoid arthritis.
