Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 3 de 3
Filter
Add more filters










Database
Language
Publication year range
1.
Biomolecules ; 11(10)2021 09 30.
Article in English | MEDLINE | ID: mdl-34680064

ABSTRACT

Hyaluronan (HA) is widely used for eye drops as lubricant to counteract dry eye disease. High and low molecular weight HA are currently used in ophthalmology. However, a large portion of the current literature on friction and lubrication addresses articular (joint) cartilage. Therefore, eye drops compositions based on HA and its derivatized forms are extensively characterized providing data on the tribological and mucoadhesive properties. The physiochemical properties are investigated in buffers used commonly in eye drops formulations. The tribological investigation reveals that amphiphilic HA-C12 decreases the friction coefficient. At the same time, the combination of trehalose/HA or HAC12 enhances up to eighty-fold the mucoadhesiveness. Thus, it is predicted a prolonged residence time on the surface of the eye. The incorporation of trehalose enhances the protection of human keratinocytes (HaCaT) cells, as demonstrated in an in-vitro cell-desiccation model. The presence of trehalose increases the friction coefficient. Medium molecular weight HA shows significantly lower friction coefficient than high molecular weight HA. This research represents a first, wide array of features of diverse HA forms for eye drops contributing to increase the knowledge of these preparations. The results here presented also provide valuable information for the design of highly performing HA-formulations addressing specific needs before preclinic.


Subject(s)
Drug Delivery Systems , Eye/drug effects , Hyaluronic Acid/pharmacology , Lubrication , Adhesiveness , Animals , Desiccation , Filtration , Friction , HaCaT Cells , Humans , Hyaluronic Acid/chemical synthesis , Hyaluronic Acid/chemistry , Mucus/drug effects , Nephelometry and Turbidimetry , Ophthalmic Solutions/pharmacology , Proton Magnetic Resonance Spectroscopy , Rheology , Sterilization , Viscosity
2.
Carbohydr Polym ; 231: 115733, 2020 Mar 01.
Article in English | MEDLINE | ID: mdl-31888823

ABSTRACT

All-trans retinoic acid (ATRA) was grafted to hyaluronan (HA) via esterification. The reaction was mediated by mixed anhydrides. A perfect control of the degree of substitution (0.5-7.5%) was obtained by varying the molar ratio of retinoic acid in the feed. The degree of substitution plays a significant role in the long-term stability. The photodegradation of HA-ATRA upon UVA irradiation resulted in ß-ionone, ß-cyclocitral and 5,6-epoxy-(E)-retinoic acid. The photostability of the conjugate had increased with the combination with morin. The chemical structure of HA-ATRA and its degradation products was elucidated using NMR spectroscopy, SEC-MALLS, and gas chromatography-mass spectrometry (GC-MS). ATRA did not loss its biological activity after conjugation, as demonstrated by gene expression. The derivative was able to penetrate across the stratum corneum. Besides, HA-ATRA downregulated the expression of anti-inflammatory interleukins 6 and 8. HA-ATRA would be expected to be used for transdermal drug delivery or cosmetics.


Subject(s)
Antioxidants/pharmacology , Hyaluronic Acid/chemistry , Skin/drug effects , Tretinoin/chemistry , Administration, Cutaneous , Anhydrides/chemistry , Animals , Antioxidants/chemistry , Esterification , Flavonoids/chemistry , Hyaluronic Acid/chemical synthesis , Hyaluronic Acid/pharmacology , Mice , NIH 3T3 Cells , Norisoprenoids/chemistry , Norisoprenoids/pharmacology , Photolysis/drug effects , Skin/radiation effects , Tretinoin/chemical synthesis , Tretinoin/pharmacology , Ultraviolet Rays
3.
Microsc Res Tech ; 79(6): 512-7, 2016 Jun.
Article in English | MEDLINE | ID: mdl-27030160

ABSTRACT

Low voltage electron microscopes working in transmission mode, like LVEM5 (Delong Instruments, Czech Republic) working at accelerating voltage 5 kV or scanning electron microscope working in transmission mode with accelerating voltage below 1 kV, require ultrathin sections with the thickness below 20 nm. Decreasing of the primary electron energy leads to enhancement of image contrast, which is especially useful in the case of biological samples composed of elements with low atomic numbers. As a result treatments with heavy metals, like post-fixation with osmium tetroxide or ultrathin section staining, can by omitted. The disadvantage is reduced penetration ability of incident electrons influencing the usable thickness of the specimen resulting in the need of ultrathin sections of under 20 nm thickness. In this study we want to answer basic questions concerning the cutting of extremely ultrathin sections: Is it possible routinely and reproducibly to cut extremely thin sections of biological specimens embedded in commonly used resins with contemporary ultramicrotome techniques and under what conditions? Microsc. Res. Tech. 79:512-517, 2016. © 2016 Wiley Periodicals, Inc.


Subject(s)
Microscopy, Electron/methods , Microtomy/methods , Plastic Embedding/methods , Animals , Epoxy Resins/chemistry , Equipment Design , Heart/diagnostic imaging , Mice , Microscopy, Electron/instrumentation , Myocardium/ultrastructure , Polymers/chemistry
SELECTION OF CITATIONS
SEARCH DETAIL
...