Comprehensive agrobiological assessment and analysis of genetic relationships of promising walnut varieties of the Nikitsky Botanical Gardens.

Walnut is an important horticultural crop, the production of which ranks second among all nut crops. Despite the significant demand in the domestic market in Russia, the industrial production of walnut fruits in Russia is currently underdeveloped. At the same time, there is a need to update the assortment with new highly productive varieties adapted to local agro-climatic conditions and having high quality nuts that are competitive at the world level. An important issue for the successful implementation of breeding programs is a comprehensive study of the gene pool. In this regard, within the framework of the study, the task was to evaluate promising varieties from the collection of the walnut gene pool of the Nikitsky Botanical Gardens and analyze genetic relationships based on microsatellite genotyping. On the basis of the performed phenotypic assessment, the study sample, which included 31 varieties, was divided into several groups according to the main phenotypic traits, such as frost and drought resistance, the start of the growing season, the ripening period, the weight and type of flowering, the weight of the fruit, and the thickness of the endocarp. Varieties with economically valuable traits that can be recommended as promising as initial parental forms in breeding work for resistance to abiotic stress factors have been identified, as well as varieties with increased productivity and large fruit sizes. Based on the analysis of eight SSR markers (WGA001, WGA376, WGA069, WGA276, WGA009, WGA202, WGA089 and WGA054), an analysis of the level of genetic diversity was performed and genetic relationships were established in the studied sample of varieties. Six (for WGA089) to eleven (for WGA276) alleles per locus have been identified. A total of 70 alleles were identified for the eight DNA markers used, with an average value of 8.75. Analysis of SSR genotyping data using Bayesian analysis established the presence of two main groups of genotypes. Taking into account the fact that all the studied varieties are selections from local seed populations in different regions of the Crimean Peninsula, the revealed level of polymorphism may indirectly reflect the level of genetic diversity of the local walnut populations. Furthermore, the presence of two genetically distant groups indicates the presence of two independently formed pools of the autochthonous gene pool of the species Juglans regia L. on the Crimean Peninsula.

Genetic structure of the population of wild-growing vines of the Utrish Nature Reserve.

Grapes are one of the most common agricultural crops in the world. Currently, the analysis of genotypes directly at the DNA level is considered to be the most accurate method for studying the plant gene pool. The study of wild vines and ancient varieties in various regions of viticulture is an important direction of research in this field. The purpose of this work was to study the population of wild grapes growing on the territory of the Utrish Nature Reserve on the Black Sea coast of Krasnodar Region. The territory of the reserve is of interest as it is a site of ancient settlements, and the environmental conditions are suitable for the growth of wild grapes. During the survey of the territory, 24 samples of wild grapes were found, which were described according to the main morphological characteristics and analyzed by the molecular genetic method. The found vines were genotyped using 15 DNA markers, including nine commonly used for DNA fingerprinting (VVS2, VVMD5, VVMD7, VVMD25, VVMD27, VVMD28, VVMD32, VrZAG62, VrZAG79) and VVIb23, which allows determining hermaphrodite and dioecious vines. Statistical processing of microsatellite loci polymorphism data was carried out using the GenAlEx 6.5 program. The genetic relationships of the studied vines were evaluated using the PAST 2.17c program. The samples were found to be morphologically and genetically polymorphic. The number of alleles identified in the sample varied from 5 to 18 and averaged 8 alleles per locus. Statistical processing of DNA analysis data made it possible to identify two genetically different populations among the wild discovered vines. An assessment of genetic similarity of the found vines with some local varieties of geographically close viticulture regions, rootstocks and representatives of Vitis sylvestris from other territories was made. One of the populations found in the Utrish Nature Reserve is close to a number of V. sylvestris genotypes, the DNA profiles of which are presented in the Vitis International Variety Catalogue.

[Otorhinolaryngological symptoms of tumors of the pharyngeal process of the parotid saliva (clinical case with literature review)]. / Otorinolaringologicheskie simptomy opukholei glotochnogo otrostka okoloushnoi slyunoi zhelezy (klinicheskii sluchai s obzorom literatury).

The parotid gland is the largest salivary gland. Tumors of the salivary gland account for 5% in the structure of oncological morbidity, and up to 80% of tumors affect the large salivary glands with tumor localization in 20% of cases in the pharyngeal process of the parotid salivary gland. The main clinical sign of a tumor of the parotid salivary gland is the presence of a slowly growing mass in the area of the gland. Often, underestimation of the clinical symptoms of salivary gland tumors is the reason for the late diagnosis of this disease and the initial manifestations of the process remain unnoticed for a long time. In order to increase the efficiency of clinical diagnostics of salivary gland tumors, the staff of the Department of Maxillofacial Surgery, Otorhinolaryngology and Topographic Anatomy with Operative Surgery of the Burdenko Voronezh State Medical University studied the clinical and anatomical parallels of changes in the ENT organs in lesions of the pharyngeal process of the parotid salivary gland. Using the previously obtained results of interdisciplinary interaction of otorhinolaryngologists and maxillofacial surgeons made it possible to increase the efficiency of diagnosis and treatment of patients with tumors of the pharyngeal process of the parotid salivary gland.

Genetic polymorphism of local Abkhazian grape cultivars.

Local grape cultivars from different countries of the world are an important part of the gene pool of this culture. Of particular interest are the genotypes of the most ancient regions of viticulture. The territories of the subtropical zone of Georgia and the central part of Abkhazia belong to one of the centers of origin of the cultural grapevine. The purpose of the work was to genotype native Abkhazian grape cultivars, to study their genetic diversity based on DNA profiling data and to compare them with the genotypes of local varieties of other viticultural regions. Samples of plants were taken on the territory of the Republic of Abkhazia in private farmsteads and in the collection of the agricultural firm "Vina i Vody Abkhazii" ("Wines and Waters of Abkhazia"). The genotyping of the Abkhazian cultivars Avasirhva, Agbizh, Azhapsh, Azhizhkvakva, Azhikvaca, Atvizh, Atyrkuazh, Achkykazh, Kachich was carried out using 14 DNA markers, 9 of which are standard microsatellite markers recommended for the identification of grape varieties. To improve our knowledge about the sizes of the identified alleles, we used the DNA of grape cultivars with a known allelic composition at the analyzed loci. Statistical analysis of the data showed that the observed heterozygosity for the analyzed loci exceeded expected values, which indicates a genetic polymorphism of the studied sample of varieties. Evaluation of genetic similarity within the analyzed group based on the results of genotyping at 14 loci showed that the cultivars Kachich and Azhapsh differed from the other Abkhazian varieties. The obtained DNA profiles of the Abkhazian cultivars were checked for compliance with DNA-fingerprints of grape varieties in the Vitis International Variety Catalogue. The Georgian varieties Azhizhkvakva and Tsitska turned out to be synonyms according to DNA profiles, two varieties from the Database (Italian Albana bianca and Georgian Ojaleshi) have differences in DNA-fingerprints from the varieties Atyrkuazh and Azhikvatsa only in one allele, respectively. When comparing the identified Abkhazian grape genotypes, their difference from the sample of Dagestan, Don, Greek, Turkish, Italian, Spanish, and French varieties and genetic similarity with the genotypes of Georgian grapes were shown.

Analysis of genetic relationships of genotypes of the genus Rosa L. from the collection of Nikita Botanical Gardens using ISSR and IRAP DNA markers.

In connection with the development of breeding and the creation of new plant varieties, the problem of their genotyping and identification is becoming increasingly important, therefore the use of molecular methods to identify genetic originality and assess plant genetic diversity appears to be relevant. As part of the work performed, informative ISSR and IRAP DNA markers promising for the study of genetic diversity of the Rosa L. genus were sought and applied to analysis of genetic relationships among 26 accessions of the genus Rosa L. from the gene pool collection of Nikita Botanical Gardens. They included 18 cultivated varieties and 8 accessions of wild species. The species sample included representatives of two subgenera, Rosa and Platyrhodon. The subgenus Platyrhodon was represented by one accession of the species R. roxburghii Tratt. Cultivated roses were represented by varieties of garden groups hybrid tea, floribunda, and grandiflora. The tested markers included 32 ISSRs and 13 IRAPs. Five ISSR markers (UBC 824, ASSR29, 3A21, UBC 864, and UBC 843) and three IRAPs (TDK 2R, Сass1, and Сass2) were chosen as the most promising. They were used for genotyping the studied sample of genotypes. In general, they appeared to be suitable for further use in studying the genetic diversity of the genus Rosa L. The numbers of polymorphic fragments ranged from 12 to 31, averaging 19.25 fragments per marker. For markers UBC 864 and UBC 843, unique fingerprints were identified in each accession studied. The genetic relationships of the studied species and varieties of roses analyzed by the UPGMA, PCoA, and Bayesian methods performed on the basis of IRAP and ISSR genotyping are consistent with their taxonomic positions. The genotype of the species R. roxburghii of the subgenus Platyrhodon was determined genetically as the most distant. According to clustering methods, the representative of the species R. bengalensis did not stand out from the group of cultivated varieties. When assessing the level of genetic similarity among the cultivated varieties of garden roses, the most genetically isolated varieties were 'Flamingo', 'Queen Elizabeth', and 'Kordes Sondermeldung'; for most of the other varieties, groups of the greatest genetic similarity were identified. This assessment reflects general trends in phylogenetic relationships, both among the studied species of the genus and among cultivated varieties.

[Evaluation of autogenous mandibular bone block resorption in horizontal alveolar ridge augmentation]. / Otsenka rezorbtsii autogennykh kostnykh blokov s vetvi nizhnei cheliusti pri gorizontal'noi augmentatsii al'veoliarnogo otrostka.

AIM: To perform a prospective evaluation of splitted bone block resorption after augmentation procedure using cone beam computed tomography, and evaluation of clinical results. MATERIAL AND METHODS: Bone blocks were harvested from the external oblique line of the mandible in 100 patients aged from 23 to 68 years, (64 females and 36 males). All of them underwent ridge augmentation using splitted bone blocks (SBB) technique. Three CBCT scans series were performed for each patient: before bone augmentation, at 14 days and 4 months after the surgical procedure. Alveolar crest dimensions were evaluated using s Ez3Dplus software ('Vatech', Seoul, Korea). RESULTS: Splitted bone blocks have shown low rate of graft resorption and high predictability. The mean horizontal and vertical bone loss between 2 and 16 weeks was 9.6%. The number of implants with insertion torque ≥35 N/cm was 94.4%. Statistical studies did not reveal significant differences by sex and age, as well as the width of the ridge before the operation. CONCLUSION: Autogenous bone blocks is a predictable option for the augmentation of the narrow alveolar crest. Splitted bone blocks provided effective alveolar restoration prior to implant placement. Further studies are needed to improve the outcomes of bone grafting.

[Surgical treatment of patients with arteriovenous head and neck angiodisplasia]. / Khirurgicheskoe lechenie bol'nykh s arteriovenoznymi angiodisplaziiami v oblasti golovy i shei.

The aim of the study was to assess the results of surgical treatment in 74 patients with extensive arteriovenous angiodisplasia in the head and neck area. All patients underwent complex examination including ultrasound, CT and MRI with contrast and selective carotid angiography. The lesions excision was performed after endovascular embolization and soft tissue defects were restored by various methods selected according to prevalent vascular lesion type (arterial, arteriovenous, venous, capillary) and blood flow values (high flow, low flow, mixed). Good and satisfactory esthetic results were seen in 95.2% of cases, lesion relapse was detected in 4.8% of cases by 12-18 months follow-up. Endovascular embolization proved to decrease the intraoperative bleeding risk, prevent lesion relapse and minimize the size of the lesion improving the results of radical treatment. Local flap reconstruction usually results in good esthetic results. By local soft tissue deficiency distant free flaps or microsurgical complex flaps may be used.

[The multidisciplinary approach to diagnostics and treatment of hypervascular masses in ENTstructures].

The objective of the present work was to improve the quality of diagnostics and treatment of hypervascular masses in ENT structures based on the multidisciplinary approach. The study involved 51 patients presenting with hypervascular masses in ENT structures. All of them underwent comprehensive medical examination including fibrolaringoscopy, multispiral computed tomography, magnetic resonance tomography, ultrasound dopplerography, selective carotid angiography, and/or various combinations of these techniques. The patients were given surgical, endovascular, and combined treatments. The study has demonstrated the advantages of application of the multidisciplinary approach to combined diagnostics and treatment of hypervascular masses in ENT structures as exemplified by neck hemangioma.

[Occupational risk management: prognosis, causation and bioinformational technologies].

Methodology of occupational risk management is outlined based on workers' health disorders forecast and causation (work-relatedness assessment). It originates from Labour Code of Russian Federation prescriptions and includes principles, methods and criteria of risk management and risk communication. The methodology is realized by means of bioinformational technologies as expert and analytical system in the form of interactive Web-based directory "occupational risk assessment" for practical use for occupational risk prevention.

[Multidisciplinary approach to diagnostics of extensive vascular head and neck malformations].

The article summarizes the experience of diagnostics in 60 patients with large face and neck angiodysplasia. Compression test, cytology, Doppler ultrasound with color Doppler mapping, X-ray computed tomography and angiography with intravenous contrast, magnetic resonance imaging with angiography and selective carotid angiography of branches of external carotid arteries were used for adequate diagnostics. The efficiency of a multidisciplinary approach in diagnosis and treatment of extensive face and neck angiodysplasias is shown in the study.

[Application of endovascular methods in complex diagnostics and treatment of tongue angiodysplasias].

In article experience of diagnostics and treatment of 30 patients with angiodysplasia of the tongue was given. The surgical treatment was made to 21 patients with vascular malformation of the tongue, selective bilateral catheterization of lingual arteries and endovascular microembolization by PVA - 250-400 µ was successfully performed in 4 cases, transcutaneus sclerotherapy with solution aethoxyskleroli 0,5% in 3 cases was successfully provided. Advantages of endovascular diagnostics and treatment methods angiodysplasias of the tongue were shown.

[Combined treatment of auricular vascular neoplasms].

Two cases of combined treatment of auricular vascular neoplasms (arteriovenous malformation and hemangioma) are presented. Their two-step management included endovascular microembolization of the posterior auricular artery with polyvinyl alcohol (PVA 300, 500 mcm) and surgical resection of the tumour. The results confirm the feasibility of combination of traditional and endovascular surgery for the treatment of this pathology. Preoperative endovascular microembilization not only arrests hemorrhage from angiodysplasia but also decreases its size and isolates it from healthy tissues. Embolization of the posterior auricular artery can be performed only after the comprehensive angiographic evaluation of the blood flow in angiodysplasia and the adjacent vessels using superselective catheterization and officinal emboli.

[Endovascular microembolization of the posterior auricular artery in comprehensive surgical management of capillary angioma of the floor of the auricle].

The article deals with the description of a rare clinical case wherein a 44-year-old female patient presenting with bleeding capillary angioma of the left auricle obstructing the auditory passage was fist subjected to endovascular microembolization of the posterior auricular artery by means of polyvinyl alcohol (PVA-500), followed by a second stage of virtually bloodlessly surgical removal of the tumour carried out five days thereafter. This case report once again points to feasibility of combined, i. e. endovascular and conventional surgical management of capillary angiomas. Preoperative microembolization of a bleeding haemangioma arrests haemorrhage from the tumour, resulting in the decreased size thereof, as well as leading to circumscribing the tumour from healthy tissue. Attention is paid to the fact that embolization of the auricular artery should be performed only after a thorough angiographic evaluation of the pattern of the blood flow in the tumour and adjacent vessels by means of the technique of selective catheterization and using only the officinal emboli.

[Insulating polymeric film in comprehensive prevention of complications of mandibular fractures]. / Ispol'zovanie izoliruiushchei polimernoi plenki v kompleksnoi profilaktike vospalitel'nykh oslozhnenii perelomov nizhnei cheliusti.

A method for prevention of inflammatory complications of mandibular fractures making use of insulating polymer-based bioactive film was studied on 48 mice. Antibacterial and osteoregeneratory activities of the therapeutic film were assessed.

[Escherichiosis in adults and the carriage of pathogenic Escherichia in some regions of Ukraine]. / Esherikhiozy u vzroslykh i nositel'stvo patogennykh ésherikhii v nekotorykh regionakh Ukrainy.

The role is shown of pathogenic Escherichieae (PE) in the development of escherichiosis in two regions with socioeconomic differences. The epidemiological picture of these infections depending on the age of the examined is analyzed. The seasonal character of circulation of pathogenic Escherichieae serogroups was found to play a major role in the etiology of escherichiosis in adults. The biological properties of the isolated strains were studied.

[The epidemiology of escherichiosis in 2 agrarian regions]. / Epidemiologiia ésherikhiozov v dvukh agrarnykh regionakh.

A study of the epidemiology of escherichiosis in two agricultural regions of the Ukraine revealed differences in the etiological structure in the limits of regions, points within a twelve-year period. It is concluded that continuous monitoring of serological pictures of pathogenic Escherichia is necessary.

[The use of an immunoenzyme method for detecting enteroviruses]. / Ispol'zovanie immunofermentnogo metoda dlia obnaruzheniia énterovirusov.

It was established as a result of these investigations that the immunoenzymatic method may be used for detection of enteroviruses both in materials from human subjects and from objects of the environment. It is shown that the immunoenzymatic method has advantages as compared with the virological.

[The epidemiology of escherichiosis at recreation areas]. / Epidemiologiia ésherikhiozov v zonakh rekreatsii.

A study of epidemiological and microbiological data (2073 epidemiological examinations of patients with escherichiosis and carriers of pathogenic Escherichia and microbiological examination of 2156 strains of pathogenic Escherichia) in two recreation zones with different types of industry (clean recreation zone and that with developed industry) revealed differences in the incidence of the escherichiosis morbidity and carrier state of Escherichia, age structure of patients and carriers, seasonal distribution of the patients and proportion of the serogroups of pathogenic Escherichia isolated in the zones. It is concluded that above findings are of importance for planning antiepidemic measures in case of escherichiosis.