ABSTRACT
The mechanism of permetrin action on two home dust mite species: Dermatophagoides farinae and D. pteronyssinus. Both species turned out to be resistant to permetrin under different conditions of toxicity evaluation. A factor of cuticle permeability was investigated. The impact of monoxygenases and esterases in the permetrin detoxication mechanism was shown. The spectra of esterase complexes of enzymes was examined by means of electrophoresis in PAAG; in addition, the zones of esterase activity were identified with the help of two substrates: 2-NA and ATI. The qualitative differences of the esterase complexes of enzymes of both species were found.
Subject(s)
Dust , Insecticides , Mites , Pyrethrins , Animals , Dose-Response Relationship, Drug , Emulsions , Esterases/drug effects , Insecticides/toxicity , Mites/enzymology , Nymph , Oxygenases/drug effects , Permethrin , Pyrethrins/toxicityABSTRACT
The present study revealed that 73% of surveyed apartments in Moscow whose residents included children with the atopic form of bronchial asthma and sensitization to Dermatophagoides pteronyssinus allergens were infested with the pyroglyphid mites D. pteronyssinus and D. farinae. The number of mites in the surveyed apartments varied between 0 and 154 mites/g of dust for D. pteronyssinus and between 0 and 162 mites/g of dust for D. farinae. The levels of mite allergens in these apartments ranged from 0.5 to 165.8 micrograms/g for Der p I and from 0.3 to 91.3 micrograms/g of dust for Der f I. The Der p I allergen was found to predominate, and its concentration in one-third of the apartments was more than 10-fold greater than that of Der f I. Correlation between the number of pyroglyphid mites and the concentration of group I allergens was established for both D. pteronyssinus (r = 0.4932; P < 0.01) and D. farinae (r = 0.6748; P < 0.01). In most of the apartments, high and moderate levels of Der I allergens were detected.
Subject(s)
Dust/analysis , Glycoproteins/analysis , Glycoproteins/isolation & purification , Mites/immunology , Animals , Antigens, Dermatophagoides , Asthma/etiology , Child , Enzyme-Linked Immunosorbent Assay , Housing , Humans , Hypersensitivity/complications , Methods , MoscowABSTRACT
Whether insect growth regulators, such as methoprene, telflubenzuron and flufenoxuron, can be used to suppress the populations of the house dust mites D. pteronyssinus and D. farinae has been studied in the laboratory setting. The most potent agent has been found to be methoprene used in concentrations of 0.05 = 0.1%, in particular. Flufenoxuron at a concentration of < 0.1% has been shown to stimulate the reproduction of house dust mites.
Subject(s)
Dust , Insecticides , Juvenile Hormones , Mites , Animals , Benzamides , Dose-Response Relationship, Drug , Emulsions , Methoprene , Phenylurea CompoundsSubject(s)
Insecticides , Mites , Aerosols , Animals , Dust , Insecticides/administration & dosage , Nymph , Time FactorsABSTRACT
Contents of histamine, 5-hydroxytryptamine, functional state of heparinic proteoglycan have been studied in the rat peritoneal mast cells during various seasons of the year (January-February, May-June, July). In winter the mast cells have a high content of histamine and 5-hydroxytryptamine, heparinic proteoglycan of their granules is stained with both alcian blue and safranin. In summer (July) content of histamine in the mast cells is sharply decreased in comparison with that of 5-hydroxytryptamine and in May-June the content of both amines is decreased nearly to background values. Both during spring and summer periods heparinic proteoglycan of the mast cell granules is stained only with alcian blue and does not take safranin. A suggestion is made on independence of the seasonal changes of annual rhythmical pattern of histamine and 5-hydroxytryptamine contents in the mast cells. A conclusion is made concerning possible participation of the mast cell system of organs and tissues in the seasonal changes of biogenic amine levels in them.
