Sonication-induced unfolding proteins.

The methodology of predicting sonication-induced unfolding proteins (SUP) is presented in this study. The methodology bases on: (a) simulation of SUP by the excessive deviations of protein domains in regime of damped forced vibrations caused by critical level of involved acoustic energy, which is associated with temperature rise and acoustic pressure; (b) simulation of stochasticity of SUP by failures in jobs service in the queueing system with Markovian fluxes. The assessments of probability of SUP accounting the complex of parameters of pulsed ultrasound, biophysical properties of tissue and macromolecular crowding of insonated zone of tissue are considered.