ABSTRACT
The paper presents new information about the carbohydrate structures of 39-days chicken's fibronectin. It is found out that chicken fibronectin contains mainly biantennary N-glycans with a core fucose and fucosylated O-glycans. It is shown that N-glycans of chicken fibronectin are poorly sialated, since this protein exhibits affinity for the PNA and weak binding to sialospecific SNA. A comparative analysis of lectin-binding activity of chicken and human fibronectins has shown that both glycoproteins differ in glycan composition.
Subject(s)
Fibronectins , Peanut Agglutinin/metabolism , Plant Lectins/metabolism , Polysaccharides/chemistry , Ribosome Inactivating Proteins/metabolism , Animals , Antibodies/metabolism , Chickens , Chromatography, Affinity , Chromatography, High Pressure Liquid , Electrophoresis, Polyacrylamide Gel , Fibronectins/blood , Fibronectins/chemistry , Fibronectins/isolation & purification , Fucose/chemistry , Glycosylation , Humans , Immunosorbent Techniques , Protein Binding , Sialic Acids/chemistry , Species SpecificityABSTRACT
The deficit of selenium is related to immunity worsening. Selenium improves an immune answer and raises disease resistance. The aim of our work was to study the influence of organic selenium in comparison with inorganic selenium on the factors of unspecific resistance and state of humoral immunity of chicken-broilers.
Subject(s)
Antibody Formation/drug effects , Dietary Supplements , Immunity, Innate/drug effects , Organoselenium Compounds/pharmacology , Selenium Compounds/pharmacology , Animals , Antigen-Antibody Complex/blood , Body Weight/drug effects , Chickens , Fibronectins/blood , Fibronectins/metabolism , Immunoglobulin G/blood , Immunoglobulin M/blood , Liver/drug effects , Liver/metabolism , Organoselenium Compounds/administration & dosage , Selenium Compounds/administration & dosageABSTRACT
Natrium humate introduction into ration of broilers activates the synthetic phase of protein exchange. The increase of protein amount in blood serum and chicken tissues, the pool decrease of free amino acids in blood and its simultaneous growth in muscles, increase of proteolysis level, the change in activity of peptide-hydrolase testify to this. In muscles the quantity of free amino acids ensuring the reamination increases considerably.
Subject(s)
Amino Acids/drug effects , Animal Feed , Chickens/metabolism , Humic Substances/administration & dosage , Amino Acids/metabolism , Animals , Humic Substances/pharmacology , Muscle Proteins/drug effects , Muscle Proteins/metabolism , Peptide Hydrolases/drug effects , Peptide Hydrolases/metabolism , Time FactorsABSTRACT
Natrium humate introduced into broiler chickens Broiler-6 line increases mass on 5-7% on the average and poultry safety on 3-5%. In chicken tissues the indexes of protein, mineral and lipid metabolism normalize, the organism's protection and adaptation opportunities improve as a result of peat preparation influence on fodder component mastering and regulatory processes.
Subject(s)
Chickens/metabolism , Humic Substances/pharmacology , Animals , Body Weight/drug effects , Chickens/immunology , Food Additives/pharmacology , Immunity, Innate/drug effects , Lipid Metabolism , Time FactorsABSTRACT
A therapeutic effect of natrium humate given to experimental mongrel rats exposed to 60Co-gamma-radiation of lethal doses has been studied. A single administration of natrium humate, 5-10 min following irradiation with a dose of 193.5 mC/kg (LD100/30) leads to a 43.3 per cent survival of animals after 60 days; with a dose of 232.2 mC/kg (LD100/8) there is a trend toward the increase in the life span of exposed rats.
