The effect of early administration of antibiotics or feeding a diet containing a coccidiostat on inflammatory responses and the morphological structure of selected organs of the immune system in young meat-type turkeys.

It was assumed that early administration of enrofloxacin or doxycycline may impair immune function and alter the morphology of organs of the immune system in turkeys, and that diets containing the coccidiostat monensin, an ionophore antibiotic, can exert similar effects. The aim of this study was to determine whether early antibiotic administration or feeding a diet containing a coccidiostat affect immune function in young turkeys. The experiment had a completely randomized design, with 8 groups (a total of 3,080 one-day-old turkeys), 7 replicate pens per group and 55 birds per pen. The experiment had a 2-factorial design, with 4 treatments (C-control, M-monensin, E-enrofloxacin, and D-doxycycline) and 2 groups of birds (vaccinated and unvaccinated) per treatment. Control group birds did not receive the coccidiostat or antibiotics. Group M was administered monensin at 90 mg/kg feed for the first 5 d of life, group E received enrofloxacin at 10 mg/kg BW, added to drinking water, for the first 5 d of life, and group D received doxycycline at 50 mg/kg BW, added to drinking water, for the first 5 d of life. One-day old turkeys from groups C+, M+, E+, and D+ were administered live-attenuated vaccines against turkey rhinotracheitis (TRT) (Poulvac TRT; Zoetis, Parsippany, NJ) and Newcastle disease (ND) (Nobilis ND clone 30; Merck, Rahway, NJ) by coarse spray; 28-day-old birds were administered a subcutaneously injected inactivated vaccine against Ornithobacterium rhinotracheale (ORT) (Ornitin, Phibro, Poland). Turkeys from groups C-, M-, E-, and D- were not vaccinated. It was found that early administration of enrofloxacin or doxycycline, or feeding a diet containing monensin, did not weaken the immune system of turkeys. The administration of monensin, in particular when combined with vaccination, was least effective in inhibiting inflammatory responses. Histological changes in immunocompetent organs (fatty degeneration) were also most severe in birds receiving monensin, followed by those administered doxycycline and enrofloxacin. The observed changes were exacerbated by vaccination.

Immune Parameters in Chickens Treated with Antibiotics and Probiotics during Early Life.

The aim of the study was to compare the effect of the administration of antibiotics or probiotics on chickens in their first week of life, on selected parameters of humoral and cellular immunity, and on the bursa of Fabricius and spleen indices. The experiment was carried out on 90 one-day-old male broilers. The control group received no additive in the drinking water; the group GP received a probiotic providing Enterococcus faecium and Bacillus amyloliquefaciens; and the group GA received 10% enrofloxacin in the drinking water on the first five days of life. Administration of the antibiotic enrofloxacin or a probiotic containing E. faecium and B. amyloliquefaciens strains to chickens in their first week of life exerts pronounced immunomodulatory effects on humoral and cellular defense mechanisms in these birds. The changes in the subpopulations of B and T cells immediately following early administration of enrofloxacin or the probiotic were not observed at the age of 35 days. Early administration of enrofloxacin can pose a risk of suppression of humoral immunity, as indicated by the significant decrease in the total IgY concentration in the plasma of the chickens.

Effect of soil contamination with polycyclic aromatic hydrocarbons from drilling waste on germination and growth of lawn grasses.

In many studies, grasses were used to increase the biodegradation of polycyclic aromatic hydrocarbons (PAHs) in soil because they are the most common plant species on the ground level and are quite resistant to contamination with these compounds. One of the main failures in PAH remediation in soil using plant species was the negative impact on germination and seedling growth. The objective of this study was to evaluate grass seed germination and seedling growth affected by drill cuttings to determine the resistance of selected grass species to the impact of PAH and their suitability for an effective phytoremediation of soils contaminated with waste that contain compounds from this group. In the study four grass species: tall fescue (Festuca arundinacea), red fescue (Festuca rubra), perennial ryegrass (Lolium perenne) and common meadow-grass (Poa pratensis). The germination energy of all species decreased as the amount of drill cuttings increased. Among the species studied, the highest germination energy and capacity were found in Lolium perenne (54.1 and 73.2 respectively), and the lowest - in Poa pratensis (16.7 and 23.3 respectively). With an increasing amount of drill cuttings, the root and seedling height were decreased. Festuca arundinacea seedlings were distinctly the highest and had the longest roots (96.7 and 52.7, respectively), while Poa pratensis seedlings showed the significantly slowest seedling and root elongation rate (30.4 and 12.4, respectively). However, the strongest decrease in seedling height and root length compared to the control was observed in Festuca rubra. Based on IC50, the greatest tolerance to the addition of drilling waste to the substrate was found for Festuca arundinacea and Festuca rubra. The conducted investigation indicates that Festuca arundinacea and Lolium perenne are grass species that are least sensitive to drilling waste in the substrate because no significant differences were found in root length and seedling height between the control soil and the soil where a PAH dose of 5% and 10% was applied.

The immune status, oxidative and epigenetic changes in tissues of turkeys fed diets with different ratios of arginine and lysine.

In the present experiment, it was assumed that the appropriate dietary ratio of arginine (Arg) to lysine (Lys) can improve the immune status and growth performance of turkeys. The aim of this study was to evaluate the effects of two inclusion rates of Arg relative to Lys in turkey diets with Lys content consistent with National Research Council (NRC) recommendations or 10% higher on the immune status of birds and indicators of protein and DNA damage due to oxidation, nitration or epigenetic changes. Another goal was to determine which dietary Arg:Lys ratio stimulates the immune response of turkeys vaccinated against Ornithobacterium rhinotracheale. The experiment was performed on 576 turkeys randomly assigned to four groups with two levels of Lys (low = NRC recommendation or high = NRC + 10%) and two levels of Arg (95% or 105% Arg relative to the content of dietary Lys). It was found that the Lys content of turkey diets should be 10% higher than that recommended by the NRC and combined with the higher Arg level (105% of Lys). Although the above Arg:Lys ratio did not improve the growth performance of birds, it stimulated their immune system and reduced protein nitration as well as protein and DNA oxidation.

The Effect of Administration of a Phytobiotic Containing Cinnamon Oil and Citric Acid on the Metabolism, Immunity, and Growth Performance of Broiler Chickens.

It was postulated that a phytobiotic preparation containing cinnamon oil and citric acid added to drinking water for chickens in a suitable amount and for a suitable time would beneficially modify the microbiota composition and morphology of the small intestine, thereby improving immunity and growth performance without inducing metabolic disorders. The aim of the study was to establish the dosage and time of administration of such a phytobiotic that would have the most beneficial effect on the intestinal histology and microbiota, production results, and immune and metabolic status of broiler chickens. The experiment was carried out on 980 one-day-old male chickens until the age of 42 days. The chickens were assigned to seven experimental groups of 140 birds each (seven replications of 20 individuals each). The control group (G-C) did not receive the phytobiotic. Groups CT-0.05, CT-0.1, and CT-0.25 received the phytobiotic in their drinking water in the amount of 0.05, 0.1, and 0.2 mL/L, respectively, at days 1-42 of life (continuous application, CT). The birds in groups PT-0.05, PT-0.5, and PT-0.25 received the phytobiotic in the same amounts, but only at days 1-7, 15-21, and 29-35 of life (periodic application, PT). Selected antioxidant and biochemical parameters were determined in the blood of the chickens, as well as parameters of immune status and redox status. The morphology of the intestinal epithelium, composition of the microbiome, and production parameters of chickens receiving the phytobiotic in their drinking water were determined as well. The addition of a phytobiotic containing cinnamon oil and citric acid to the drinking water of broiler chickens at a suitable dosage and for a suitable time can beneficially modify the microbiome composition and morphometry of the small intestine (total number of fungi p < 0.001, total number of aerobic bacteria p < 0.001; and total number of coliform bacteria p < 0.001 was decreased) improving the immunity and growth performance of the chickens (there occurred a villi lengthening p = 0.002 and crypts deepening p = 0.003). Among the three tested dosages (0.05, 0.1, and 0.25 mL/L of water) of the preparation containing cinnamon oil, the dosage of 0.25 mL/L of water administered for 42 days proved to be most beneficial. Chickens receiving the phytobiotic in the amount of 0.25 mL/L had better growth performance, which was linked to the beneficial effect of the preparation on the microbiome of the small intestine, metabolism (the HDL level p = 0.017 was increased; and a decreased level of total cholesterol (TC) p = 0.018 and nonesterified fatty acids (NEFA) p = 0.007, LDL p = 0.041, as well as triacylglycerols (TAG) p = 0.014), and immune (the level of lysozyme p = 0.041 was increased, as well as the percentage of phagocytic cells p = 0.034, phagocytosis index p = 0.038, and Ig-A level p = 0.031) and antioxidant system (the level of LOOH p < 0.001, MDA p = 0.002, and the activity of Catalase (CAT) p < 0.001 were decreased, but the level of ferric reducing ability of plasma (FRAP) p = 0.029, glutathione p = 0.045 and vitamin C p = 0.021 were increased).

Selected Alkaloids Used in the Cosmetics Industry.

Plants are a rich source of a wide variety of bioactive compounds that can be used for the preparation of cosmetics. Natural cosmetics with plant components such as vitamins, polyphenols, and alkaloids have become more and more popular. Alkaloids are important secondary metabolites in plants. They are known to possess therapeutic properties. Alkaloids can be used in the production of tonics, creams, lotions, face and hair masks, compresses for skin problems with numerous inflammations, and discoloration and antiaging products, as well as for reducing the formation of cellulitis. Alkaloids are also used in the production of ampoules for cosmetologists and aesthetic medicine doctors. However, at higher doses, they may exhibit toxic properties. Several studies have been carried out in evaluation of the activity of alkaloids from various plants for their use in cosmetics. This review describes alkaloids (caffeine, capsaicin, berberine, piperine, spilanthol, and anatabine) derived from various plants that are used in cosmetics, as well as their reported activities.

The effect of different dietary ratios of lysine, arginine and methionine on biochemical parameters and hormone secretion in turkeys.

We postulated that the use of optimal levels and proportions of Lys, Arg and Met in compound feed allows for optimal exploitation of the growth potential of contemporary slaughter turkey hybrids and reduces metabolic disorders. The aim of the study was to determine the effect of different proportions of Lys, Arg and Met in diets whose Lys content is in accordance with NRC recommendations, that is a low level, on selected parameters of protein, lipid and carbohydrate metabolism and on hormone secretion in turkeys. The lowest Arg content (90% Lys) in the diet resulted in an increase in plasma total cholesterol levels in the turkeys as compared to higher Arg content (100% or 110% of Lys), (2.50 vs. 2.09 vs. 1.83). Plasma HDL and creatinine concentration increased in turkeys fed diets with higher Arg content (100% and 110% Lys) compared to turkeys receiving the diet with the lowest Arg content (90% Lys). Compared to turkeys receiving the lowest and intermediate Arg content (90% and 100% Lys), the diet with the highest content of this AA (110% Lys) resulted in an increase in the plasma T4 level (71.21 vs. 86.60 vs. 128.2). The varied Arg and Met levels relative to Lys did not affect the secretion of neurotransmitters or hormones regulating glucose metabolism. At low levels of Met in the diet, an decrease in Arg relative to Lys from 100% to 90% caused a growth depression of turkeys (10.68 vs. 10.21 kg), which was not noted in the case of the higher Met content. When using the Lys level recommended by NRC in the turkey diet, the optimal Arg level is 100% and Met is 45% compared to Lys.

Oxidative and Epigenetic Changes and Gut Permeability Response in Early-Treated Chickens with Antibiotic or Probiotic.

The aim of this study was to compare the effect of the use of enrofloxacin and a probiotic containing Enterococcus faecium and Bacillus amyloliquefaciens strains in the first week of life of chickens on oxidative and epigenetic changes in molecules and intestinal integrity. The three treatments were as follows: the control group received no additive in the drinking water (GC); the second group (GP) received a probiotic preparation in the drinking water during the first five days of life, providing E. faecium strain 4a1713 at 1.0 × 107 CFU/L water and B. amyloliquefaciens 4b1822 at 1.0 × 107 CFU/L water, the third group (GA) received an antibiotic (enrofloxacin 0.5 mL/L water) in the drinking water during the first five days of life. The use of both enrofloxacin and a probiotic containing E. faecium and B. amyloliquefaciens strains in chickens' first week of life improved intestinal integrity and reduced inflammation and oxidative and epigenetic changes in the small intestine. This effect was evident both at 6 days of age and at the end of the rearing period.

Estimated intestinal absorption of phosphorus and its deposition in chosen tissues, bones and feathers of chickens receiving chromium picolinate or chromium nanoparticles in diet.

The aim of the study was to determine whether the level and form of Cr in the diet of chickens influences its accumulation in tissues as well as intestinal absorption of P and its deposition in tissues. The experiment was carried out on 405 one-day-old male Ross 308 chickens that were randomly divided into five treatment groups. Control group was fed the diet without supplemental chromium; experimental groups were fed the diet with 3 or 6 mg/kg chromium picolinate (Cr-Pic) and with 3 or 6 mg/kg chromium nanoparticles (Cr-NP). Chromium was found to accumulate in the tissues of the ileum, liver, breast muscle, bones skin and in feathers of chickens. Chromium deposited in the ileum of chickens does not affect the ex vivo estimated intestinal absorption of P. The use of Cr in the diet of chickens carries the risk of lowering P levels in femur.

Antioxidant Status and Liver Function of Young Turkeys Receiving a Diet with Full-Fat Insect Meal from Hermetia illucens.

We hypothesized that full-fat insect meal from Hermetia illucens (HI) larvae can be an acceptable source of protein and energy in the diet of young turkeys, in an amount adapted to the nutritional needs of these birds, and at the same time can improve their antioxidant status and metabolism. The turkeys were fed a control diet (HI0) without the insect meal, and three diets with increasing HI content of 5%, 10% and 15% (treatments HI5, HI10 and HI15, respectively). The use of 10% or 15% HI in the diet of young turkeys, while beneficially raising levels of P, Fe and Hb, has a negative effect on lipid metabolism, increasing TC levels, lipid oxidation, and fat deposition in the liver. The inclusion of 5% HI in the diet of young turkeys has no adverse effect on the lipid status and histology of the liver, but it does not improve antioxidant status. To conclude, the level of HI meal in the diet of turkeys should not exceed 5%. However, as similar studies on turkeys have not yet been published, overly general conclusions should not be drawn from the results of the present study, and further research is necessary.

The Effect of Chromium Nanoparticles and Chromium Picolinate in the Diet of Chickens on Levels of Selected Hormones and Tissue Antioxidant Status.

: We have postulated that supplementation with Cr can increase serotonin levels and improve the antioxidant status of chickens, with no adverse effect on the secretion of other hormones. The study aimed to determine what form and dose of Cr more favorably affect the level of selected hormones (insulin, glucagon, serotonin, dopamine, noradrenaline, histamine, T3 and T4) and the antioxidant status (level of malondialdehyde and lipid peroxides, activity of superoxide dismutase and catalase) of chicken tissues. The experiment was carried out on chickens randomly divided into five treatment groups. The basal diets (control group) were supplemented with two levels of Cr (3 and 6 mg/kg) and two Cr sources: Cr-picolinate (Cr-Pic) and Cr-nano (Cr-NP) to obtain four experimental diets: 3 mg/kg Cr-Pic, 6.0 mg/kg Cr-Pic, 3.0 mg/kg Cr-NP. and 6.0 mg/kg Cr-NP. The addition of Cr in both forms increased the level of serotonin at a dose of 3 mg/kg and, at the same time, reduced the level of noradrenaline. The addition of Cr at 3 mg/kg, irrespective of the form used, regulated the level of hormones of carbohydrate metabolism (increasing insulin levels and reducing glucagon levels) and had an adverse effect on the antioxidant status of the liver and breast muscle. Due to the adverse effect of Cr at 3 mg/kg on the antioxidant status of chickens, this level of Cr should not be considered in both forms Cr-Pic and Cr-NP as a feed additive for broiler chickens. In the future, studies on the potential beneficial effects of Cr on the organism should take into account doses lower than 3 mg/kg.

Effect of Different Levels and Sources of Dietary Copper, Zinc and Manganese on the Performance and Immune and Redox Status of Turkeys.

The aim of the study was to determine the effectiveness of the combined use of Cu, Zn, and Mn nanoparticles in the diet of turkeys with regard to potential reduction of the levels of these elements added to feed. The experiment was carried out on turkeys' hens assigned to four groups. Turkeys from the PC group received feed with the addition of inorganic forms of Cu, Zn, and Mn in the B.U.T. (British United Turkeys) recommended levels, from group IR received the addition in amounts reduced to 10% of the recommended levels, and from group NR received the addition of elements in the form of nanoparticles in the same amounts as in group IR. The turkeys from group NC received feed without the addition of these elements. The research showed that the addition of Cu, Zn, and Mn to turkey diets, in both inorganic forms and as nanoparticles, in quantities covering only 10% of B.U.T. recommendations had no adverse effect on growth performance or on the antioxidant and immune defense of turkeys. The changes in the redox status of the turkeys whose diet was not supplemented with Cu, Zn, and Mn indicate reduced oxidation processes in the tissues.

The effect of the source and dose of manganese on the performance, digestibility and distribution of selected minerals, redox, and immune status of turkeys.

The aim of this study was to determine the effect of various levels of manganese added to the diet of growing turkeys in the conventional form of MnO or in the form of NP-Mn2O3 nanoparticles on growth performance, absorption, and accumulation of Mn, Zn, and Cu, and antioxidant and immune status. The experiment was conducted on 1080 one-day-old Hybrid Converter turkeys randomly assigned to 6 groups with 10 replications, in a two-factor design with three dosages of manganese - 100, 50, and 10 mg/kg, and two sources-manganese oxide (MnO) and manganese nanoparticles (NP-Mn2O3). Neither reducing the addition of Mn from 100 to 50 or even 10 mg/kg of the diet nor replacing MnO with NP-Mn2O3 had a negative effect on the growth performance of the turkeys. Replacing MnO with NP-Mn2O3 in the turkey diet improved ileal digestibility of Mn and decreased accumulation of Cu in the liver and breast muscle. The study showed that irrespective of the form used, reducing the level of Mn supplementation of the diet from the 100 mg/kg recommended by British United Turkey to 50 or 10 mg/kg decreased its ileal digestibility and increased its accumulation in the liver, breast muscle, and skin. Reducing the addition of Mn to the turkey diet increased intestinal absorption of Zn and reduced accumulation of Zn and Cu in the liver, breast muscle, and skin. It did not increase oxidation processes in the liver or breast muscle of the turkeys. Reducing the addition of Mn to the turkey diet stimulated the immune system, which was manifested by stimulation of B cells to produce immunoglobulin M and by the release of the cytokine IL-6, but did not intensify apoptosis. The results of the study indicate that the recommended manganese supplement in turkey diets can be reduced. The use of manganese nanoparticles in turkey feeding requires further study.

The effect of manganese nanoparticles on apoptosis and on redox and immune status in the tissues of young turkeys.

The aim of the study was to determine whether the use of Mn nanoparticles would make it possible to reduce the level of this micronutrient added to turkey diets without adversely affecting growth performance, antioxidant and immune status, or apoptosis. The experiment was conducted on 6 groups of turkeys with 10 replications in a two-factor design with 3 dosages of manganese, 100, 50 and 10 mg/kg, and 2 sources, manganese oxide (MnO) and manganese nanoparticles (NP-Mn2O3). The study showed that irrespective of the form of Mn used, reducing the Mn level recommended by British United Turkeys for supplementation of the diet of young turkeys from 100 mg/kg to 10 mg/kg increases the content of this element in the blood with no adverse effect on growth performance or the immune system. The reduction in Mn supplementation in the form of NP-Mn2O3 from 100 to 50 and even to 10 mg/kg of turkey diet has no negative effect on antioxidant defence in young turkeys. A 50% reduction of the recommended Mn level in the form of MnO enhances lipid oxidation processes. Replacing MnO with NP-Mn2O3 in the turkey diet probably can increase apoptosis in young turkeys. On the other hand, irrespective of the form of Mn used, reducing supplementation of the turkey diet with this element from 100 to 50 and even to 10 mg/kg probably can reduce apoptosis.

Effect of nanoparticles of silver on redox status and the accumulation of Ag in chicken tissues.

BACKGROUND: The aim of the study was to verify the hypothesis that there are doses of silver nanoparticles (Ag-NPs) that improve antioxidant defence in chickens without compromising their growth performance and health. RESULTS: The results of our study suggest that, irrespective of the concentration, the administration of Ag-NPs (5 nm) at a dose not exceeding 54 mg/bird has no negative effect on the growth performance of chickens. The administration of Ag-NPs (5 nm) in the amount of 2.87 to 63.74 mg/bird does not cause silver to accumulate in the breast muscle. The ingestion of 2.87 mg/bird was found to result in the accumulation of this element in the wall of the small intestine and in the liver, and a further increase in the dose increases the accumulation of Ag in these tissues. Accumulation of Ag in the heart of the chickens was not observed until the dose reached 22.5 mg/bird. CONCLUSION: Our research has shown that the application of Ag-NPs (5 nm), especially at doses greater than the 'no observable adverse effect level' (NOAEL) of 9.47 mg/bird, can induce oxidative reactions in the blood, small intestinal wall, liver and breast muscle of chickens. © 2018 Society of Chemical Industry.

A simple stripping voltammetric method for the determination of a new anticancer prodrug in serum.

The determination of ethyl [4-oxo-8-(3-chlorophenyl)-4,6,7,8-tetrahydroimidazo[2,1-c][1,2,4]triazin-3-yl]acetate (ETTA), a new anticancer prodrug, using adsorptive stripping voltammetry (AdSV) was described for the first time. This method is based on adsorptive/reductive behaviour of ETTA at an in situ plated bismuth film electrode (BiFE) as a sensor. A number of experimental variables (e.g., a composition and pH of the supporting electrolyte, the conditions of bismuth film deposition, an accumulation potential and time, the scan rate, etc.) were thoroughly studied in order to achieve a high sensitivity. Experimental results under optimized conditions revealed an excellent linear correlation between the monitored voltammetric peak current and the ETTA concentration in the range of 2-50µgL-1 following an accumulation time of 300s. The limit of detection (LOD) for ETTA following 300s of an accumulation time was 0.4µgL-1. The proposed facile, sensitive and inexpensive method was successfully applied to the determination of ETTA in serum. The investigated prodrug was extracted from serum using SPE method.

The effect of administration of copper nanoparticles to chickens in drinking water on estimated intestinal absorption of iron, zinc, and calcium.

Copper nanoparticles used as a dietary supplement for poultry could affect the absorption of mineral elements. Hence the aim of the study was to determine the effect of administration of copper nanoparticles to chickens in drinking water on intestinal absorption of iron, zinc, and calcium. The experiment was carried out on 126 chicks assigned to seven experimental groups of 18 birds each (3 replications of 6 individuals each). The control group (G-C) did not receive copper nanoparticles. Groups: Cu-5(7), Cu-10(7), and Cu-15(7) received gold nanoparticles in their drinking water in the amounts of 5 mg/L for group Cu-5(7), 10 mg/L for group Cu-10(7), and 15 mg/L for group Cu-15(7) during 8 to 14, 22 to 28, and 36 of 42 days of the life of the chicks. The birds in groups Cu-5(3), Cu-10(3), and Cu-15(3) received copper nanoparticles in the same amounts, but only during 8 to 10, 22 to 24, and 36 to 38 days of life. Blood for analysis was collected from the wing vein of all chicks at the age of 42 days. After the rearing period (day 42), six birds from each experimental group with body weight similar to the group average were slaughtered. The carcasses were dissected and samples of the jejunum were collected for analysis of absorption of selected minerals. Mineral absorption was tested using the in vitro gastrointestinal sac technique. Oral administration of copper nanoparticles to chickens in the amount of 5, 10, and 15 mg/L led to accumulation of this element in the intestinal walls. The highest level of copper nanoparticles applied increased Cu content in the blood plasma of the birds. The in vitro study suggests that copper accumulated in the intestines reduces absorption of calcium and zinc, but does not affect iron absorption.

Determination of Cr(VI) in the presence of high excess of a Cr(III) by adsorptive stripping voltammetry.

A sensitive and highly selective adsorptive stripping voltammetric procedure for determination of traces of Cr(VI) is presented. Cr(III) interference was minimized due to (a) partial adsorption of this form of chromium from the sample on aluminum oxide and (b) using as a detection method adsorptive stripping voltammetry, which allows for selective determination of Cr(VI) in the presence of a 100-fold excess of Cr(III). The procedure can be used for determination of Cr(VI) even in the presence of a 10,000-fold excess of Cr(III). Conditions of Cr(III) adsorption on aluminum oxide were optimized while conditions of Cr(VI) determination were based on literature data. The calibration graph for Cr(VI) for an accumulation time of 30s was linear from 1×10(-9) to 4×10(-8)molL(-1). The detection limit was 7×10(-10)molL(-1). The proposed procedure was applied for Cr(VI) determination in a river water sample.