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1.
Bioresour Technol ; 169: 510-517, 2014 Oct.
Article in English | MEDLINE | ID: mdl-25086436

ABSTRACT

A new concept is proposed for combined fermentation (two-stage high-load fermenter) and gasification (two-stage fluidised bed gasifier with CO2 separation) of sewage sludge and wood, and the subsequent utilisation of the biogenic gases in a hybrid power plant, consisting of a solid oxide fuel cell and a gas turbine. The development and optimisation of the important processes of the new concept (fermentation, gasification, utilisation) are reported in detail. For the gas production, process parameters were experimentally and numerically investigated to achieve high conversion rates of biomass. For the product gas utilisation, important combustion properties (laminar flame speed, ignition delay time) were analysed numerically to evaluate machinery operation (reliability, emissions). Furthermore, the coupling of the processes was numerically analysed and optimised by means of integration of heat and mass flows. The high, simulated electrical efficiency of 42% including the conversion of raw biomass is promising for future power generation by biomass.


Subject(s)
Biomass , Biotechnology/methods , Electricity , Fermentation , Gases/chemistry , Models, Theoretical , Biofuels , Carbon Dioxide/analysis , Computer Simulation , Sewage/chemistry , Silage , Volatilization , Wood/chemistry , Zea mays/chemistry
2.
Int J Food Microbiol ; 136(1): 75-82, 2009 Nov 30.
Article in English | MEDLINE | ID: mdl-19783060

ABSTRACT

Spontaneous fermented sourdoughs prepared from five amaranth flours were investigated for the presence of lactic acid bacteria predominating the autochthonous microbiota and thus may be suitable as starter cultures. The doughs were fermented with daily back-slopping on a laboratory scale at 30 degrees C for 10 days. Each day, pH-values and total titratable acidity degrees were determined and samples were analyzed for lactic acid bacteria and yeasts by cultural methods. The identity of the strains was tracked with randomly amplified polymorphic DNA-PCR during fermentation. Taxonomic identity of the strains was revealed by sequence analysis of 16S rDNA. Sugar and organic acid profiles of fermented doughs were determined with HPLC. The strains Lactobacillus plantarum RTa12, L. sakei RTa14, and Pediococcus pentosaceus RTa11 were selected and applied as starters in laboratory scale fermentations. All strains were predominant in repeated experiments, both as single strains and in combination, regardless of the amaranth flour used. The competitiveness of the strains L. plantarum RTa12 and P. pentosaceus RTa11 was characterized in further growth experiments. Both strains facilitated fast declines of pH-values, overgrew the autochthonous microbiota, and allowed stable fermentation characteristics at different temperatures. Thus, the characterized strains may be considered as candidates for amaranth sourdough starter cultures.


Subject(s)
Amaranthus/metabolism , Amaranthus/microbiology , Bacteria/genetics , Bacteria/isolation & purification , Fermentation , Food Microbiology , Bacteria/growth & development , Bacteriological Techniques , Colony Count, Microbial , Flour/microbiology , Hydrogen-Ion Concentration , Lactic Acid/metabolism , RNA, Ribosomal, 16S/genetics
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