COVID-19 Vaccine Provider Availability and Vaccination Coverage Among Children Aged 5-11 Years - United States, November 1, 2021-April 25, 2022.

COVID-19 can lead to severe outcomes in children, including multisystem inflammatory syndrome, hospitalization, and death (1,2). On November 2, 2021, the Advisory Committee on Immunization Practices issued an interim recommendation for use of the BNT162b2 (Pfizer-BioNTech) vaccine in children aged 5-11 years for the prevention of COVID-19; however, vaccination coverage in this age group remains low (3). As of June 7, 2022, 36.0% of children aged 5-11 years in the United States had received ≥1 of COVID-19 vaccine (3). Among factors that might influence vaccination coverage is the availability of vaccine providers (4). To better understand how provider availability has affected COVID-19 vaccination coverage among children aged 5-11 years, CDC analyzed data on active COVID-19 vaccine providers and county-level vaccine administration data during November 1, 2021-April 25, 2022. Among 2,586 U.S. counties included in the analysis, 87.5% had at least one active COVID-19 vaccine provider serving children aged 5-11 years. Among the five assessed active provider types, most counties had at least one pharmacy (69.1%) or public health clinic (61.3%), whereas fewer counties had at least one pediatric clinic (29.7%), family medicine clinic (29.0%), or federally qualified health center (FQHC)* (22.8%). Median county-level vaccination coverage was 14.5% (IQR = 8.9%-23.6%). After adjusting for social vulnerability index (SVI)† and urbanicity, the analysis found that vaccination coverage among children aged 5-11 years was higher in counties with at least one active COVID-19 vaccine provider than in counties with no active providers (adjusted rate ratio [aRR] = 1.66). For each provider type, presence of at least one provider in the county was associated with higher coverage; the largest difference in vaccination coverage was observed between counties with and without pediatric clinics (aRR = 1.37). Ensuring broad access to COVID-19 vaccines, in addition to other strategies to address vaccination barriers, could help increase vaccination coverage among children aged 5-11 years.

Wet shells and dry tales: the evolutionary 'Just-So' stories behind the structure-function of biominerals.

The ability of evolution to shape organic form involves the interactions of multiple systems of constraints, including fabrication, phylogeny and function. The tendency to place function above everything else has characterized some of the historical biological literature as a series of 'Just-So' stories that provided untested explanations for individual features of an organism. A similar tendency occurs in biomaterials research, where features for which a mechanical function can be postulated are treated as an adaptation. Moreover, functional adaptation of an entire structure is often discussed based on the local characterization of specimens kept in conditions that are far from those in which they evolved. In this work, environmental- and frequency-dependent mechanical characterization of the shells of two cephalopods, Nautilus pompilius and Argonauta argo, is used to demonstrate the importance of multi-scale environmentally controlled characterization of biogenic materials. We uncover two mechanistically independent strategies to achieve deformable, stiff, strong and tough highly mineralized structures. These results are then used to critique interpretations of adaptation in the literature. By integrating the hierarchical nature of biological structures and the environment in which they exist, biomaterials testing can be a powerful tool for generating functional hypotheses that should be informed by how these structures are fabricated and their evolutionary history.

Psychometric Evaluation of the Creighton Competency Evaluation Instrument in a Population of Working Nurses.

BACKGROUND AND PURPOSE: This study explored the psychometric properties of the Creighton Competency Evaluation Instrument (C-CEI), previously validated for use with nursing students, to assess simulation performance among registered nurses working 12-hour shifts. Valid and reliable measurements are needed to test clinical and simulation competencies and characterize the effects of fatigue on nursing performance. METHODS: Trained raters scored nurses' patient care performance in simulation scenarios using the C-CEI. We analyzed the instrument's principal components, internal reliability, and construct validity. RESULTS: Internal reliability of the C-CEI aggregate score and the Clinical Decision-making component were high (>.70). The latter robustly correlated with predicted cognitive effectiveness, a measure of fatigue. CONCLUSIONS: The C-CEI is a reliable measure for use among registered nurses and its further development will be important for testing performance of working nurses and fatigue-mitigation innovations.

The effects of three consecutive 12-hour shifts on cognition, sleepiness, and domains of nursing performance in day and night shift nurses: A quasi-experimental study.

BACKGROUND: Healthcare systems have widely adopted consecutive 12 h day and night shifts for nurses, but the effects of these shifts on cognition, sleepiness, and nursing performance remains understudied. OBJECTIVE: To determine the extent of changes in cognition and sleepiness in nurses working three consecutive 12 h shifts, quantify the respective impacts of these changes on different aspects of nursing performance, and investigate individual differences in all measures. DESIGN: A quasi-experimental, between-within design collected data from nurses between November 2018 and March 2020. The between-groups component was comprised of day shift nurses vs. night shift nurses, while the within-groups component was comprised of two separate test sessions for each nurse: one immediately following a third consecutive shift (fatigued) and one after three consecutive days off work (rested). SETTING: Participants were tested in a northwestern US university's nursing simulation laboratory. PARTICIPANTS: A volunteer sample of 94 registered nurses involved in direct patient care working 12 h shifts were recruited from two local hospitals. METHODS: Simulated nursing performance was measured in seven separate domains and an aggregate score from the Creighton Competence and Evaluation Inventory, covering both lower- and higher-level constructs like procedural skills, assessment, decision-making, etc. Cognition and sleepiness were assessed through measures of sustained attention, predicted cognitive effectiveness, and subjective sleepiness. RESULTS: In our 94 nurses, individual differences in all our measures varied from trivial to extensive. For six domains of performance and the aggregate score there were no significant differences in means across groups or conditions. For the seventh, Communication skills were lower for night nurses than day nurses, but this effect was small. After three consecutive shifts, sustained attention and predicted cognitive effectiveness decreased, and subjective sleepiness increased. Predicted cognitive effectiveness was particularly low for fatigued night nurses relative to other conditions and was positively correlated with Communication while controlling for other predictors. CONCLUSIONS AND RELEVANCE: Nurses maintained their levels of performance for all domains after three consecutive shifts. Individual differences in predicted cognitive effectiveness could account for variation in performance by shift type for Communication skills but for no other domain of performance. Communication skills and predicted cognitive effectiveness may interest researchers in the development of fatigue-mitigation strategies for night nurses, but our findings also suggest that more sensitive measures of performance may be necessary to capture other meaningful effects of long, consecutive shifts-if any-on patient care. Tweetable abstract: The effects of three consecutive 12 h shifts on cognition, sleepiness, and domains of nursing performance in day and night shift nurses: A quasi-experimental study.

Measurable reflection in simulation: A pilot study.

PURPOSE: Debriefing is considered a cornerstone in the success of simulated learning practices. For debriefing to be productive and meaningful, reflection of the learners is essential. Measuring reflection during debriefing has proven to be difficult. This study aimed to validate a reflection rubric based on Kolb's Theory of Experiential Learning and integrate the assessment tool into simulation debriefing practice. METHODS: This research was a non-experimental mixed-methods, sequential explanatory design. Validation for the rubric was accomplished by expert content review and an observation process. Students participated in a standardized patient simulation (SPS) portraying a patient with bipolar disorder. The SPS debriefings were recorded and thematically coded to determine the quality and level of reflection of the students according to the following rubric criteria based on Kolb's theory: (1) Concrete Experience, (2) Reflective Observation, (3) Abstract Conceptualism, and (4) Active Experimentation. A descriptive statistical analysis of student responses was applied. RESULTS: The Debriefing Reflection Rubric was successful in determining the level of reflection in the dental hygiene students; further descriptive results were evaluated by a thematic analysis of student responses (N = 173). Student responses demonstrated reflective observations and concrete experiences (n = 112); however, dental hygiene students demonstrated limited responses (n = 61) to abstract conceptualism and connecting their experience to future goals and experimentation. CONCLUSION: The Debriefing Reflection Rubric, based on Kolb's Theory of Experiential Learning, was successful in measuring a learner's level and quality of reflection during a simulation debriefing.

Typhoid Fever in the US Pediatric Population, 1999-2015: Opportunities for Improvement.

BACKGROUND: Typhoid fever in the United States is acquired primarily through international travel by unvaccinated travelers. There is currently no typhoid vaccine licensed in the United States for use in children <2 years. METHODS: We reviewed Salmonella enterica serotype Typhi infections reported to the Centers for Disease Control and Prevention (CDC) and antimicrobial-resistance data on Typhi isolates in CDC's National Antimicrobial Resistance Monitoring System from 1999 through 2015. RESULTS: 5131 cases of typhoid fever were diagnosed and 5004 Typhi isolates tested for antimicrobial susceptibility. Among 1992 pediatric typhoid fever patients, 1616 (81%) had traveled internationally within 30 days of illness onset, 1544 (81%) of 1906 were hospitalized (median duration, 6 days; range, 0-50), and none died. Forty percent (799) were <6 years old; 12% were <2 years old. Based on age and travel destination, 1435 (83%) of 1722 pediatric patients were vaccine-eligible; only 68 (5%) of 1361 were known to be vaccinated. Of 2003 isolates tested for antimicrobial susceptibility, 1216 (61%) were fluoroquinolone-nonsusceptible, of which 272 (22%) were also resistant to ampicillin, chloramphenicol, and trimethoprim-sulfamethoxazole (multidrug-resistant [MDR]). All were susceptible to ceftriaxone and azithromycin. MDR and fluoroquinolone-nonsusceptible isolates were more common in children than adults (16% vs 9%, P < .001, and 61% vs 54%, P < .001, respectively). Fluoroquinolone nonsusceptibility was more common among travel-associated than domestically acquired cases (70% vs 17%, P < .001). CONCLUSIONS: Approximately 95% of currently vaccine-eligible pediatric travelers were unvaccinated, and antimicrobial-resistant infections were common. New public health strategies are needed to improve coverage with currently licensed vaccines. Introduction of an effective pretravel typhoid vaccine for children <2 years could reduce disease burden and prevent drug-resistant infections.

Glyphosate Toxicity to Native Nontarget Macrophytes Following Three Different Routes of Incidental Exposure.

A major goal of invasive plant management is the restoration of native biodiversity, but effective methods for invasive plant control can be harmful to native plants. Informed application of control methods is required to reach restoration goals. The herbicide glyphosate, commonly applied in invasive plant management, can be toxic to native macrophytes. Our study assessed the response of 2 macrophytes that are endangered in our study area (Ammannia robusta and Sida hermaphrodita) to glyphosate concentrations that mimic incidental exposure from nearby invasive plant control: spray drift of 4 × 10-7 % to 5% glyphosate; pulse and continuous immersion in water containing 2 to 41 µg/L glyphosate; and rhizosphere contact with 5%-glyphosate-wicked invasive plants. We assessed macrophyte sensitivity at 14-d postexposure, and quantified abundance of arbuscular mycorrhizal fungi. Glyphosate spray concentrations as low as 0.1% reduced macrophyte growth. Ammannia was more sensitive overall to glyphosate spray than Sida, although sensitivity varied among measured endpoints. Conversely, macrophytes were not affected by immersion in low concentrations of glyphosate or rhizosphere contact with a glyphosate-wicked plant. Likewise, arbuscular mycorrhizal fungi abundance in roots was similar among glyphosate-sprayed and control plants. Based on our results, we recommend that invasive plant managers reduce risks to native nontarget plants through implementing measures that limit off-target spray drift, and consider the feasibility of more targeted applications, such as with wick equipment. Integr Environ Assess Manag 2021;17:597-613. © 2020 The Authors. Integrated Environmental Assessment and Management published by Wiley Periodicals LLC on behalf of Society of Environmental Toxicology & Chemistry (SETAC).

Verdet constant of potassium terbium fluoride crystal as a function of wavelength and temperature.

Potassium terbium fluoride KTb3F10 (KTF) crystal is a promising magneto-active material for creating multi-kilowatt average-power Faraday isolators operating at the visible and near-infrared wavelengths. Nevertheless, the key material's parameter needed for the design of any Faraday isolator-the Verdet constant, has not been comprehensively investigated yet. In this Letter, we report on measurement of the Verdet constant of the KTF crystal for wavelengths between 600 and 1500 nm and for temperatures ranging from 15 to 295 K. A suitable model for the Verdet constant as a function of wavelength and temperature has been developed and may be conveniently used for optimal design of KTF-based high-average-power Faraday isolators.

The palaeobiology of belemnites - foundation for the interpretation of rostrum geochemistry.

Belemnites are an extinct group of Mesozoic coleoid cephalopods with a fossil record ranging from the early Late Triassic [about 240 million years ago (Mya)] to the Cretaceous/Palaeogene boundary (65 Mya). Belemnites were widely distributed, highly abundant and diverse, and an important component of Mesozoic marine food webs. Their internal shells, specifically their low-Mg calcite rostra, have been used as palaeoenvironmental carbonate archives for the last 70 years. This is primarily due to the assumption that the rostrum calcite formed in equilibrium with the oxygen isotope composition of ambient sea water. Of prime importance for the reliable interpretation of isotope data derived from these biogenic carbonates is a robust reconstruction of the palaeobiology of their producers. Here we provide a critical assessment of published reconstructions of belemnite soft-body organization and their lifestyle and habitats. Different lines of evidence, including sedimentological, geochemical, morphological, and biomechanical data, point towards an outer shelf habitat of belemnites, for some taxa also including the littoral area. Belemnite habitat temperatures, oxygen content, salinities, and life span are constrained based on observations of the ecology and life history of modern coleoids. Belemnite habitat depth might have been largely controlled by food and temperature, with a temperature optimum between 10°C and 30°C. The distribution of modern coleoids is for most species restricted to well-oxygenated water masses and a salinity between 27 and 37 psu. The trophic position of belemnites as both predators and prey is documented by unique fossil finds of stomach contents and soft tissue preservation, such as jaws, hooks, and ink sacs. Belemnites were medium-sized predators in the epipelagic zone (not deeper than ∼200 m) hunting for crustaceans, other cephalopods, and fishes. Taxa with elongated rostra probably were fast and highly manoeuvrable swimmers. Forms with conical rostra represent slow but highly manoeuvrable swimmers, and forms with depressed rostra likely had a bottom-related life habit. Predators of adult belemnites were sharks, bony fishes, and marine reptiles. Belemnites, like most of the modern coleoids, were relatively short lived, most likely living only for 1-2 years. Understanding the biomineralization of belemnite rostra is highly relevant for an improved interpretation of their geochemistry. Here we confirm that belemnite rostra are composed of low Mg-calcite fibres, but they do not contain distinct types of laminae. These fibres are composed of two distinct calcite phases. One phase is a filigree network of tetrahedral organic-rich calcite and the second phase is represented by organic-poor calcite.

Large-scale polymeric carbon nanotube membranes with sub-1.27-nm pores.

We report the first characterization study of commercial prototype carbon nanotube (CNT) membranes consisting of sub-1.27-nm-diameter CNTs traversing a large-area nonporous polysulfone film. The membranes show rejection of NaCl and MgSO4 at higher ionic strengths than have previously been reported in CNT membranes, and specific size selectivity for analytes with diameters below 1.24 nm. The CNTs used in the membranes were arc discharge nanotubes with inner diameters of 0.67 to 1.27 nm. Water flow through the membranes was 1000 times higher than predicted by Hagen-Poiseuille flow, in agreement with previous CNT membrane studies. Ideal gas selectivity was found to deviate significantly from that predicted by both viscous and Knudsen flow, suggesting that surface diffusion effects may begin to dominate gas selectivity at this size scale.

Characterization and extinction measurement of potassium terbium fluoride single crystal for high laser power applications.

High optical quality of potassium terbium fluoride, KTb3F10 (KTF), crystals are characterized and tested for extinction at high laser powers up to 400 W. The measured Verdet constant of the crystal is comparable with that of terbium gallium garnet (TGG) crystal. KTF crystals show high extinction at high incident power and an order of magnitude smaller absorption with respect to TGG, making it a superior crystal for high laser power applications.

Optical properties of lithium terbium fluoride and implications for performance in high power lasers.

LiTbF4 has the potential to replace traditional magneto-optic garnet materials as a Faraday rotator in high power laser systems due to its high Verdet constant. New measurements are reported of the ordinary and extraordinary refractive indices of LiTbF4 as functions of wavelength and temperature, respectively, as well as their corresponding Sellmeier expressions. Consequently, the Verdet coefficient was calculated and plotted as a function of wavelength and temperature. These measurements will aid in further development of LiTbF4 as an optical isolator.

Use of a Mobile Device Simulation as a Preclass Active Learning Exercise.

BACKGROUND: Research shows that preclass activities introducing new material can increase student performance. In an effort to engage students in an active learning, preclass activity, the authors developed a mobile application. METHOD: Eighty-four nursing students were assigned a preclass reading exercise, whereas 32 students completed the preclass simulation scenario on their mobile device. All students completed the same electronic fetal monitoring (EFM) quiz 1 week following the lecture. The effects of reading or simulation on student quiz performance was evaluated with a student's paired t test, using an alpha of .05. RESULTS: Students completing the preclass simulation scored higher on the EFM quiz, compared with students assigned the preclass reading (85% versus 70% correct answers, p = .01). Student survey data indicated that the mobile device simulation was perceived as an engaging and desirable instructional tool. CONCLUSION: Nursing students completing the mobile device EFM preclass simulation outperformed the students who were given the traditional reading assignment.

Highly CO2-Selective Gas Separation Membranes Based on Segmented Copolymers of Poly(Ethylene oxide) Reinforced with Pentiptycene-Containing Polyimide Hard Segments.

Poly(ethylene oxide) (PEO)-containing polymer membranes are attractive for CO2-related gas separations due to their high selectivity toward CO2. However, the development of PEO-rich membranes is frequently challenged by weak mechanical properties and a high crystallization tendency of PEO that hinders gas transport. Here we report a new series of highly CO2-selective, amorphous PEO-containing segmented copolymers prepared from commercial Jeffamine polyetheramines and pentiptycene-based polyimide. The copolymers are much more mechanically robust than the nonpentiptycene containing counterparts due to the molecular reinforcement mechanism of supramolecular chain threading and interlocking interactions induced by the pentiptycene structures, which also effectively suppresses PEO crystallization leading to a completely amorphous structure even at 60% PEO weight content. Membrane transport properties are sensitively affected by both PEO weight content and PEO chain length. A nonlinear correlation between CO2 permeability with PEO weight content was observed due to the competition between solubility and diffusivity contributions, whereby the copolymers change from being size-selective to solubility-selective when PEO content reaches 40%. CO2 selectivities over H2 and N2 increase monotonically with both PEO content and chain length, indicating strong CO2-philicity of the copolymers. The copolymer film with the longest PEO sequence (PEO2000) and highest PEO weight content (60%) showed a measured CO2 pure gas permeability of 39 Barrer, and ideal CO2/H2 and CO2/N2 selectivities of 4.1 and 46, respectively, at 35 °C and 3 atm, making them attractive for hydrogen purification and carbon capture.

The effects of triclosan on spore germination and hyphal growth of the arbuscular mycorrhizal fungus Glomus intraradices.

The effect of triclosan (5-chloro-2-[2,4-dichlorophenoxy]phenol; TCS), on spore germination, hyphal growth, and hyphal branching of the arbuscular mycorrhizal (AM) fungus, Glomus intraradices spores was evaluated at exposure concentrations of 0.4 and 4.0 µg/L in a static renewal exposure system. To determine if potential effects were mycotoxic or a consequence of impaired signaling between a host plant and the fungal symbiont, spores were incubated with and without the addition of a root exudate. Exposed spores were harvested at days 7, 14, and 21. AM spore germination, hyphal growth, and hyphal branching were significantly lower in both TCS concentrations compared to controls in non-root exudate treatments suggesting direct mycotoxic effects of TCS on AM development. Greater hyphal growth and hyphal branching in controls and 0.4µg/L TCS treatments with root exudate compared to non-root exudate treatments demonstrated growth stimulation by signaling chemicals present in the root exudate. This stimulatory effect was absent in the 4.0 µg/L TCS treatments indicating a direct effect on plant signaling compounds or plant signal response.

Triclosan inhibits arbuscular mycorrhizal colonization in three wetland plants.

In terrestrial ecosystems, plant growth, plant community structure, and ultimately the ecosystem services provided by plants are dependent on the presence and composition of below ground arbuscular mycorrhizal (AM) fungal communities. AM fungi form obligate symbioses with plants providing nutrients to their host plants in exchange for photosynthates. While AM have been found in most wetland ecosystems, the effects of urban contaminants on AM associations are largely unknown. Triclosan (5-chloro-2-[2,4-dichlorophenoxy]phenol; TCS) is a widespread contaminant found in surface waters throughout North America and in addition to antimicrobial properties is purported to have antifungal properties. To determine the effects of TCS on arbuscular mycorrhizal associations, we exposed AM inoculated wetland plant species (Eclipta prostrata, Hibiscus laevis, and Sesbania herbacea) to TCS at concentrations of 0.0, 0.4 and 4.0 µg/L in a continuous flow-through exposure system. TCS exposure caused significant reductions in hyphal and arbuscular colonization while no significant effect was detected for vesicular colonization. Across all species, hyphal colonization was significantly higher in controls (18.58 ± 1.84%) compared to 0.4 and 4.0 µg/L (10.20 ± 1.34% and 9.86 ± 1.32% respectively) TCS treatments. Similarly, arbuscular colonization was significantly higher in the controls (4.58 ± 0.75%) compared to 0.4 µg/L (2.20 ± 0.38%) and 4.0 µg/L (1.22 ± 0.24%) TCS exposures. Since our lowest effect concentration, 0.4 µg/L, lies within the range of concentrations found in North American streams it is plausible that AM colonization has been impacted in streams receiving WWTP effluent. Further studies are required to understand the mechanism of TCS inhibition of mycorrhizal colonization in wetland plant species as well as the potential ecological consequences that a decline in the AM colonization levels may represent.

Improving nursing students' knowledge using online education and simulation to help smokers quit.

UNLABELLED: The purpose of this study was to examine an evidence-based smoking cessation education program effect on nursing students' perceptions and self-confidence to help smokers quit smoking. METHOD: Upon completion of an on-line educational program students were provided opportunities to practice through simulation. Data were collected by pre and post assessment tools for the online-education and by a post assessment tool for the simulation. RESULTS: 110 senior nursing students participated in this study. Students' confidence in their overall ability to assist smokers to quit smoking was statistically significant compared to baseline (t=4.09, p<.001). Students self-rated higher levels of smoking cessation skills on Advising, Assessing, Assisting, and Arranging compared to baseline (p<0.00). CONCLUSION: Integration of evidence-based didactic and practical smoking cessation program into curricula improves nursing students' clinical skills in helping smokers to quit.

Bioconcentration of triclosan, methyl-triclosan, and triclocarban in the plants and sediments of a constructed wetland.

Constructed wetlands are a potential method for the removal of two pharmaceutical and personal care products from wastewater effluent. Triclosan (TCS; 5-chloro-2-[2,4-dichlorophenoxy]phenol) and triclocarban (TCC; 3,4,4'-trichlorocarbanillide) are antimicrobial agents added to a variety of consumer products whose accumulation patterns in constructed wetlands are poorly understood. Here, we report the accumulation of TCS, its metabolite methyl-triclosan (MTCS; 5-chloro-2-[2,4-dichlorophenoxy]), and TCC in wetland plant tissues and sediments. Three wetland macrophytes: Typha latifolia, Pontederia cordata, and Sagittaria graminea were sampled from a constructed wetland in Denton, Texas, USA. MTCS concentrations were below the method detection limit (MDL) for all species. TCS root tissue concentrations in T. latifolia were significantly greater than root concentrations in P. cordata (mean±SE in ng g(-1): 40.3±11.3 vs. 15.0±1.9, respectively), while for TCC, shoot tissue concentrations in S. graminea were significantly greater than in T. latifolia (22.8±9.3 vs. 9.0 (MDL), respectively). For both TCS and TCC, T. latifolia root tissue concentrations were significantly greater than shoot concentrations (TCS: 40.3±11.3 vs. 17.2±0.2, TCC: 26.0±3.6 vs. 9.0, (MDL)). TCC concentrations in P. cordata roots were significantly greater than in shoots (34.4±5.3 vs. 15.4±2.8, respectively). TCS concentrations in T. latifolia roots and sediments and TCC concentrations in sediments generally decreased from wetland inflow to outflow. To our knowledge, this is the first study documenting species and tissue specific differences in the accumulation of TCS and TCC in plants from an operational constructed wetland. The species specific differences in bioaccumulation suggest TCS and TCC removal from constructed wetlands could be enhanced through targeted plantings.

Effects of arbuscular mycorrhizal fungi on seedling growth and development of two wetland plants, Bidens frondosa L., and Eclipta prostrata (L.) L., grown under three levels of water availability.

To identify the importance of arbuscular mycorrhizal fungi (AMF) colonizing wetland seedlings following flooding, we assessed the effects of AMF on seedling establishment of two pioneer species, Bidens frondosa and Eclipta prostrata grown under three levels of water availability and ask: (1) Do inoculated seedlings differ in growth and development from non-inoculated plants? (2) Are the effects of inoculation and degree of colonization dependent on water availability? (3) Do plant responses to inoculation differ between two closely related species? Inoculation had no detectable effects on shoot height, or plant biomass but did affect biomass partitioning and root morphology in a species-specific manner. Shoot/root ratios were significantly lower in non-inoculated E. prostrata plants compared with inoculated plants (0.381 ± 0.066 vs. 0.683 ± 0.132). Root length and surface area were greater in non-inoculated E. prostrata (259.55 ± 33.78 cm vs. 194.64 ± 27.45 cm and 54.91 ± 7.628 cm(2) vs. 46.26 ± 6.8 cm(2), respectively). Inoculation had no detectable effect on B. frondosa root length, volume, or surface area. AMF associations formed at all levels of water availability. Hyphal, arbuscular, and vesicular colonization levels were greater in dry compared with intermediate and flooded treatments. Measures of mycorrhizal responsiveness were significantly depressed in E. prostrata compared with B. frondosa for total fresh weight (-0.3 ± 0.18 g vs. 0.06 ± 0.06 g), root length (-0.78 ± 0.28 cm vs.-0.11 ± 0.07 cm), root volume (-0.49 ± 0.22 cm(3) vs. 0.06 ± 0.07 cm(3)), and surface area (-0.59 ± 0.23 cm(2) vs.-0.03 ± 0.08 cm(2)). Given the disparity in species response to AMF inoculation, events that alter AMF prevalence in wetlands could significantly alter plant community structure by directly affecting seedling growth and development.