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1.
Tuberculosis (Edinb) ; 88(1): 64-8, 2008 Jan.
Article in English | MEDLINE | ID: mdl-17980664

ABSTRACT

In a prospective study conducted by laboratory technologists in a diagnostic laboratory in Cape Town, South Africa, a semi-automated phage-based antibiotic susceptibility assay was implemented and the performance of the luciferase reporter mycobacteriophage (LRP) system for susceptibility testing of clinical Mycobacterium tuberculosis complex (MTC) isolates against rifampin and isoniazid was evaluated. Two hundred consecutive clinical MGIT cultures of MTC species were included in this study. Antibiotic susceptibility assays were set up manually for the LRP and BACTEC radiometric systems (BACTEC) and read in a plate luminometer and the BACTEC 460 instrument, respectively. Discrepant susceptibility results were resolved by the conventional agar proportion method. Of the 200 secondary cultures prepared for this study, 9 (4.5%) were lost to contamination (LRP 4, BACTEC 1, both 4). All of the remaining 191 cultures underwent susceptibility testing by both methods and the overall agreement between the LRP and BACTEC was 98.4% (rifampin 100%; isoniazid 96.9%). Of the 6 discrepant cultures tested by the agar proportion method, 2 gave results in agreement with the LRP. The sensitivity of the LRP for detection of drug-resistant isolates was 100% for both rifampin (n=9) and isoniazid (n=12). The median turnaround time for susceptibility testing was 2 days with the LRP and 9 days with BACTEC. In conclusion, the semi-automated LRP-based assay offers a rapid and practical approach for accurate susceptibility testing of M. tuberculosis cultures in diagnostic laboratories with limited financial resources, but with competent technologists.


Subject(s)
Antitubercular Agents/pharmacology , Genes, Reporter , Luciferases/genetics , Microbial Sensitivity Tests/methods , Mycobacteriophages/genetics , Mycobacterium tuberculosis/drug effects , Tuberculosis/diagnosis , Biological Assay , Humans , Isoniazid/pharmacology , Mycobacteriophages/physiology , Mycobacterium tuberculosis/virology , Prospective Studies , Rifampin/pharmacology , South Africa
2.
J Interferon Cytokine Res ; 26(1): 27-33, 2006 Jan.
Article in English | MEDLINE | ID: mdl-16426145

ABSTRACT

The role of tumor necrosis factor-alpha (TNF-alpha) in controlling growth of Mycobacterium tuberculosis in murine peritoneal macrophages infected in vitro was studied. TNF-alpha was shown to be required but not sufficient, and the amount of TNF-alpha produced by the infected cells did not correlate with the extent of growth control. In this system, TNF-alpha-dependent control of growth of the avirulent strain H37Ra was independent of inducible nitric oxide synthase (iNOS) and interferon-gamma (IFN-gamma), as shown by the infection of macrophages from selected gene-disrupted mice. TNF-alpha-mediated bacteriostasis of H37Ra in the infected macrophages was associated with increased expression of selected Th1-type cytokines and chemokines. In contrast, growth of the virulent strain H37Rv in macrophages involved upregulation by infected cells of Th2-type cytokines, including interleukin-5 (IL-5), IL-10, and IL-13. Taken together, these results suggest that the particular nature of macrophage activation and the cytokine and chemokine response to infection with different M. tuberculosis strains determine the ability of the cells to control the growth of the intracellular bacilli.


Subject(s)
Chemokines/immunology , Cytokines/immunology , Macrophages, Peritoneal/immunology , Macrophages, Peritoneal/microbiology , Mycobacterium tuberculosis/immunology , Animals , Cells, Cultured , Chemokines/genetics , Cytokines/genetics , In Vitro Techniques , Macrophage Activation , Macrophages, Peritoneal/cytology , Mice , Mice, Inbred C57BL , Mice, Knockout , Nitric Oxide Synthase Type II/genetics , Nitric Oxide Synthase Type II/metabolism , Tumor Necrosis Factor-alpha/immunology
3.
Biotechnol Lett ; 27(3): 173-9, 2005 Feb.
Article in English | MEDLINE | ID: mdl-15717126

ABSTRACT

The effect on translation of downstream box sequences optimized for binding to Mycobacterium smegmatis and Escherichia coli 16S rRNA in the absence of a Shine-Dalgarno (SD) region was investigated. The relative translational efficiency of each construct in either M. smegmatis or E. coli was determined. Eradication of the SD region in the absence of a downstream box abolished the translation activity. In contrast, optimized downstream box constructs resulted in a 13- and 18-fold increase in protein synthesis, relative to non-optimized DB controls in E. coli and M. smegmatis, respectively.


Subject(s)
Escherichia coli/genetics , Mycobacterium smegmatis/genetics , Protein Biosynthesis , Base Sequence , Binding Sites/genetics , Electroporation , Gene Expression Regulation, Bacterial , Molecular Sequence Data , Plasmids/genetics , RNA, Ribosomal, 16S/genetics , RNA, Ribosomal, 16S/metabolism , Sequence Alignment
4.
J Infect Dis ; 190(1): 99-106, 2004 Jul 01.
Article in English | MEDLINE | ID: mdl-15195248

ABSTRACT

Multidrug-resistant tuberculosis (MDR-TB) is a major public health problem because treatment is complicated, cure rates are well below those for drug-susceptible tuberculosis (TB), and patients may remain infectious for months or years, despite receiving the best available therapy. To gain a better understanding of MDR-TB, we characterized serial isolates recovered from 13 human immunodeficiency virus-negative patients with MDR-TB, by use of IS6110 restriction fragment-length polymorphism analysis, spacer oligonucleotide genotyping (i.e., "spoligotyping"), and sequencing of rpoB, katG, mabA-inhA (including promoter), pncA, embB, rpsL, rrs, and gyrA. For all 13 patients, chronic MDR-TB was caused by a single strain of Mycobacterium tuberculosis; 8 (62%) of the 13 strains identified as the cause of MDR-TB belonged to the W-Beijing family. The sputum-derived isolates of 4 (31%) of the 13 patients had acquired additional drug-resistance mutations during the study. In these 4 patients, heterogeneous populations of bacilli with different resistance mutations, as well as mixtures of drug-susceptible and drug-resistant genotypes, were observed. This genetic heterogeneity may require treatment targeted at both drug-resistant and drug-susceptible phenotypes.


Subject(s)
Antitubercular Agents/pharmacology , Drug Resistance, Multiple, Bacterial/genetics , Mycobacterium tuberculosis/classification , Mycobacterium tuberculosis/drug effects , Polymorphism, Genetic , Tuberculosis, Multidrug-Resistant/microbiology , Adult , Antitubercular Agents/therapeutic use , Bacterial Proteins/genetics , Chronic Disease , DNA Transposable Elements/genetics , Female , Humans , Male , Microbial Sensitivity Tests , Middle Aged , Mutation , Mycobacterium tuberculosis/genetics , Mycobacterium tuberculosis/isolation & purification , Polymorphism, Restriction Fragment Length , Sequence Analysis, DNA , Sputum/microbiology , Tuberculosis, Multidrug-Resistant/drug therapy
5.
Infect Immun ; 71(12): 7099-108, 2003 Dec.
Article in English | MEDLINE | ID: mdl-14638800

ABSTRACT

Protective immunity against pulmonary tuberculosis (TB) is characterized by the formation in the lungs of granulomas consisting of macrophages and activated T cells producing tumor necrosis factor alpha and gamma interferon, both required for the activation of the phagocytes. In 90% of immunocompetent humans, this response controls the infection. To understand why immunity fails in the other 10%, we studied the lungs of six patients who underwent surgery for incurable TB. Histologic examination of different lung lesions revealed heterogeneous morphology and distribution of acid-fast bacilli; only at the surface of cavities, i.e., in granulomas with a patent connection to the airways, were there numerous bacilli. The mutation profile of the isolates suggested that a single founder strain of Mycobacterium tuberculosis may undergo genetic changes during treatment, leading to acquisition of additional drug resistance independently in discrete physical locales. Additional drug resistance was preferentially observed at the cavity surface. Cytokine gene expression revealed that failure to control the bacilli was not associated with a generalized suppression of cellular immunity, since cytokine mRNA was up regulated in all lesions tested. Rather, a selective absence of CD4(+) and CD8(+) T cells was noted at the luminal surface of the cavity, preventing direct T-cell-macrophage interactions at this site, probably allowing luminal phagocytes to remain permissive for bacillary growth. In contrast, in the perinecrotic zone of the granulomas, the two cell types colocalized and bacillary numbers were substantially lower, suggesting that in this microenvironment an efficient bacteriostatic or bactericidal phagocyte population was generated.


Subject(s)
Lung/immunology , Lung/pathology , Mycobacterium tuberculosis/growth & development , Tuberculosis, Pulmonary/immunology , Tuberculosis, Pulmonary/pathology , Adolescent , Adult , Chronic Disease , Cytokines/genetics , Cytokines/metabolism , Female , Granuloma, Respiratory Tract/microbiology , Humans , Lung/microbiology , Macrophages/immunology , Male , Middle Aged , Mycobacterium tuberculosis/classification , Mycobacterium tuberculosis/genetics , Nitric Oxide Synthase/genetics , Nitric Oxide Synthase/metabolism , Nitric Oxide Synthase Type II , Polymorphism, Restriction Fragment Length , T-Lymphocytes/immunology , Thorax/microbiology , Tuberculosis, Pulmonary/microbiology , Tuberculosis, Pulmonary/surgery
7.
Microbiology (Reading) ; 146 ( Pt 9): 2267-2275, 2000 Sep.
Article in English | MEDLINE | ID: mdl-10974114

ABSTRACT

The alpha replicons of the multi-replicon plasmids pGSH500 and pLV1402 have been characterized by DNA sequence analysis. Analysis of the DNA sequence of a 3672 bp HIN:dIII fragment from pFDT100, which contains the pGSH500 alpha replicon, revealed similarity to a number of replicons belonging to, or related to, those of the IncFII family. The replicon region contains copA, tapA, repA and oriR, and replication initiation and termination sites are related to those from the IncFII replicon of R1. A copB gene was found to lie upstream of the HIN:dIII site in the parental plasmid pGSH500. Downstream of oriR, a 707 bp region shows 72.6% identity to a region of the Escherichia coli chromosome at 43.3', suggesting this region of pGSH500 may have been incorporated into the plasmid during a past chromosomal recombination event. Oligonucleotide primers homologous to consensus regions in the copB and repA genes, and the oriR regions from a number of IncFII-related replicons were used to amplify replication regions from pLV1402. Analysis of the amplified regions has shown the presence of copB, copA, tapA and repA genes. Phylogenetic analysis of Rep protein sequences from the RepFIIA family of antisense-control-regulated replicons revealed the presence of three distinct subgroups of Rep proteins. Comparative analysis of DNA and protein sequences from members of the RepFIIA family provides evidence supporting the roles of both non-selective divergence in co-integrate (multi-replicon) plasmids and Chi-mediated-recombination in replicon evolution, and in particular, that such processes may have been widespread in the evolution of the RepFIIA family.


Subject(s)
DNA Helicases , DNA-Binding Proteins , Evolution, Molecular , Genetic Variation , Plasmids/genetics , Recombination, Genetic , Replicon/genetics , Trans-Activators , Base Sequence , Chromosomes, Bacterial/genetics , Cloning, Molecular , DNA, Bacterial/chemistry , DNA, Bacterial/genetics , Escherichia coli/genetics , Molecular Sequence Data , Nucleic Acid Conformation , Proteins/genetics , RNA, Antisense/metabolism , Replication Origin/genetics , Sequence Analysis, DNA
8.
Microbiology (Reading) ; 145 ( Pt 9): 2507-2518, 1999 Sep.
Article in English | MEDLINE | ID: mdl-10517603

ABSTRACT

An Escherichia coli-mycobacterial shuttle vector, pJCluc, containing a luciferase reporter gene, was constructed and used to analyse the Mycobacterium tuberculosis katG promoter. A 1.9 kb region immediately upstream of katG promoted expression of the luciferase gene in E. coli and Mycobacterium smegmatis. A smaller promoter fragment (559 bp) promoted expression with equal efficiency, and was used in all further studies. Two transcription start sites were mapped by primer extension analysis to 47 and 56 bp upstream of the GTG initiation codon. Putative promoters associated with these show similarity to previously identified mycobacterial promoters. Deletions in the promoter fragment, introduced with BAL-31 nuclease and restriction endonucleases, revealed that a region between 559 and 448 bp upstream of the translation initiation codon, designated the upstream activator region (UAR), is essential for promoter activity in E. coli, and is required for optimal activity in M. smegmatis. The katG UAR was also able to increase expression from the Mycobacterium paratuberculosis P(AN) promoter 15-fold in E. coli and 12-fold in M. smegmatis. An alternative promoter is active in deletion constructs in which either the UAR or the katG promoters identified here are absent. Expression from the katG promoter peaks during late exponential phase, and declines during stationary phase. The promoter is induced by ascorbic acid, and is repressed by oxygen limitation and growth at elevated temperatures. The promoter constructs exhibited similar activities in Mycobacterium bovis BCG as they did in M. smegmatis.


Subject(s)
Bacterial Proteins , Gene Expression Regulation, Bacterial , Mycobacterium tuberculosis/genetics , Peroxidases/genetics , Peroxidases/metabolism , Promoter Regions, Genetic/genetics , Base Sequence , DNA Primers , DNA, Bacterial/analysis , Escherichia coli/genetics , Gene Deletion , Genetic Vectors , Molecular Sequence Data , Mycobacterium smegmatis/enzymology , Mycobacterium smegmatis/genetics , Mycobacterium smegmatis/growth & development , Mycobacterium tuberculosis/enzymology , Mycobacterium tuberculosis/growth & development , Polymerase Chain Reaction/methods , RNA, Bacterial/analysis , RNA, Bacterial/isolation & purification , Sequence Analysis, DNA , Transcription, Genetic , Transformation, Bacterial
9.
Microbiology (Reading) ; 145 ( Pt 8): 2011-2021, 1999 Aug.
Article in English | MEDLINE | ID: mdl-10463167

ABSTRACT

The Mycobacterium tuberculosis KatG enzyme, like most hydroperoxidase I (HPI)-type catalases, consists of two related domains, each with strong similarity to the yeast cytochrome c peroxidase. The catalase-peroxidase activity is associated with the amino-terminal domain but currently no definite function has been assigned to the carboxy-terminal domain, although it may play a role in substrate binding. This paper reports another possible function of the KatG protein involving protection of the host cell against DNA-damaging agents. The M. tuberculosis katG gene, the 5' domain and the 3' domain were cloned separately, in-frame with the maltose-binding protein, into the vector pMAL-c2. These constructs were introduced into four DNA-repair mutants of Escherichia coli, DK1 (recA), AB1884 (uvrC), AB1885 (uvrB) and AB1886 (uvrA), which were then tested for their ability to survive treatment with UV light (254 nm), hydrogen peroxide (1.6 mg ml-1) and mitomycin C (6 micrograms ml-1). All three constructs conferred resistance to UV upon the recA E. coli cells, whereas resistance to mitomycin C was found in all repair mutants tested. Protection against hydrogen peroxide damage was less pronounced and predominantly found in the recA host. These results indicated that the M. tuberculosis katG gene can enhance DNA repair in E. coli, and that the 5' and 3' domains can function separately. UV sensitivity tests on Mycobacterium intracellulare and M. tuberculosis strains mutant in katG revealed that the katG gene product does not play an additive role in the survival of mycobacterial cells after exposure to short-wavelength UV irradiation, in repair-competent cells.


Subject(s)
ATP-Binding Cassette Transporters , Bacterial Proteins , DNA Repair/genetics , Escherichia coli Proteins , Escherichia coli/physiology , Monosaccharide Transport Proteins , Mycobacterium tuberculosis/enzymology , Peroxidases/genetics , Peroxidases/physiology , Carrier Proteins/genetics , Carrier Proteins/metabolism , Cloning, Molecular , Escherichia coli/drug effects , Escherichia coli/genetics , Escherichia coli/radiation effects , Genes, Bacterial , Genetic Vectors , Hydrogen Peroxide/pharmacology , Maltose-Binding Proteins , Mitomycin/pharmacology , Molecular Sequence Data , Mutation , Mycobacterium avium Complex/genetics , Mycobacterium avium Complex/radiation effects , Mycobacterium tuberculosis/genetics , Mycobacterium tuberculosis/radiation effects , Peroxidases/chemistry , Polymerase Chain Reaction , Protein Structure, Tertiary , Restriction Mapping , Ultraviolet Rays
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