ABSTRACT
Eastern equine encephalitis virus (EEEV) is an Alphavirus that is endemic in the Southeastern United States. From 1993 to January 2005, the Veterinary Diagnostic and Investigational Laboratory in Tifton, Georgia, performed postmortem examinations on over 101 domestic canines exhibiting clinical neurological disturbances. In 12 of these dogs, brains were histologically suggestive of infection with EEEV. All dogs were less than 6 months of age, with no breed predilection. Clinical signs included pyrexia, depression, nystagmus, and lateral recumbency. Microscopically, brains from all 12 puppies contained infiltrates of lymphocytes, plasma cells, and histiocytes, with occasional neutrophils and random foci of astrocytosis and gliosis. There were mild to moderate perivascular infiltrates of neutrophils along with scattered lymphocytes, plasma cells, and macrophages in the meninges. Viruses isolated from brain homogenates of all 12 puppies were confirmed by indirect fluorescent antibody testing to be EEEV. Additionally, RNA extracted from the brains and viral cultures of 2 dogs were determined by a specific reverse-transcriptase polymerase chain reaction (RT-PCR) to contain EEEV. The single available serum sample exhibited a 1:8 serum neutralization titer to EEEV.
Subject(s)
Dog Diseases/virology , Encephalitis Virus, Eastern Equine/isolation & purification , Encephalomyelitis/veterinary , Encephalomyelitis/virology , Animals , Brain/pathology , Dog Diseases/diagnosis , Dog Diseases/epidemiology , Dog Diseases/pathology , Dogs , Encephalomyelitis/diagnosis , Encephalomyelitis/epidemiology , Georgia/epidemiologyABSTRACT
Two dead, captive green anacondas (Eunectes murinus), including one male and one female, submitted for necropsy were in poor body condition, having multiple, scattered, dark red foci on the scales and mottled lungs. Both snakes had severe mycotic dermatitis. In addition, the male snake had mycotic stomatitis, and the female snake had mycotic pneumonia. Trichophyton sp., Verticillium sp., and Alternaria sp. were isolated from the dermal lesions. The pulmonary lesions were morphologically consistent with Aspergillus sp. Bacterial organisms isolated from skin and internal organs included Chryseobacterium meningosepticum, Stenotrophomonas maltophilia, Aeromonas hydrophila, and Providencia rettgeri. Mycotic diseases can be devastating to reptiles, and suboptimal husbandry and captivity were likely the predisposing factors that led to opportunistic invasion in these snakes.
Subject(s)
Boidae/microbiology , Dermatomycoses/veterinary , Fungi/isolation & purification , Lung Diseases, Fungal/veterinary , Alternaria/isolation & purification , Animal Husbandry/methods , Animals , Animals, Zoo , Aspergillus/isolation & purification , Dermatomycoses/diagnosis , Dermatomycoses/pathology , Fatal Outcome , Female , Fungi/classification , Fungi/pathogenicity , Kidney/pathology , Lung/microbiology , Lung/pathology , Lung Diseases, Fungal/diagnosis , Lung Diseases, Fungal/pathology , Male , Skin/microbiology , Skin/pathology , Spleen/pathology , Trichophyton/isolation & purification , Verticillium/isolation & purificationABSTRACT
Salmonella gastroenteritis and septicemia were diagnosed in two cats presented for necropsy. Both cats resided in the same household and were fed a home-prepared, raw meat-based diet. Salmonella was isolated from multiple organs in both cats and from samples of raw beef incorporated into the diet fed to one of the cats. Subtyping of the bacterial isolates yielded Salmonella newport from one cat and from the diet it had been fed. This report provides evidence that the practice of feeding raw meat-based diets to domestic cats may result in clinical salmonellosis.
