Phase-stepping holographic moiré: simultaneous in-plane and out-of-plane displacement measurement.

A new use for the phase-stepping technique in holographic moiré is demonstrated. The method has the advantage of providing instantaneous determination of in-plane and out-of-plane displacements without the necessity for auxiliary carrier fringes.

Phase-stepping automatic fringe analysis in holographic moiré.

The phase-stepping technique is used to analyze holographic moiré patterns. This new application permits the instantaneous determination of phase functions in both moiré and carrier fringes. Two modified phase-stepping self-calibrating techniques are proposed and demonstrated on computergenerated noisy moiré patterns. The techniques do not complicate the holographic moiré arrangement and do not need auxiliary carrier fringes. A holographic moiré interferometer that has been adapted for these phase-stepping techniques is proposed.

[Isotachophoretic isolation of IgD and IgE and production of monospecific antisera against them]. / Vydelenie metodom izotakhoforeza IgD i IgE i poluchenie k nim monospetsificheskikh antisyvorotok.

The method of preparative isotachophoresis in acrylamide gel ensuring a high yield of IgD and IgE with insignificant admixtures of IgG, etc. was used for the isolation of IgD and IgE from the blood sera of myeloma patients. As a result of immunization with these antigens, monospecific IgD and IgE antisera were obtained. These antisera, alongside with specific antibodies, contained antibodies to admixtures; the latter were eliminated by the method of immune absorbtion carried out with the use of a sorbent based on Sepharose activated with bromo-cyanogen and conjugated with normal human blood serum. Ig D antisera were also shown to contain antibodies to idiotypical IgD determinants located in the Fab fragment of this immunoglobulin.