ABSTRACT
A comparative study has revealed the identity of the amino acid composition of the peptide part of peptidoglycans obtained from the intact cells (the first method) and of the amino acid composition of peptidoglycans isolated from cell walls (the second method). This evidences for the possibility of using the first method when determining types of peptidoglycans for diagnosis of the coryneform bacteria genera.
Subject(s)
Actinomycetales/analysis , Amino Acids/analysis , Peptidoglycan/analysisABSTRACT
A large number of bacterial strains assimilating chemical ethanol has been isolated using an original technique. Active growth of strains belonging to the genera Pseudomonas and, particularly, Acinetobacter was registered on mineral media containing ethanol. A mathematical model was constructed select a strain of Acinetobacter calcoaceticus K-9 during its continuous cultivation on media containing ethanol. The model makes it possible to determine conditions for producing a present amount of the biomass, the percentage of its yield, and the produc;iveness as a function of the dilution rate, temperature, and the concentration of ethanol and phosphoric acid in the medium. The main characteristics of the growth process in the studied factor space were established. The optimum conditions were calculated for growth of the strain with respect to each of the criteria. Under various conditions of bacterial growth, changes in the morphology and ultra-fine structure of the cells correlated with their physiological activity. The volume of the cells increased with the rate of dilution of the medium: the process can be described by a saturation curve. The presence of mesosomal structures is typical of the cells growing at low flow rates.
