Low uptake of pharmaceuticals in edible mushrooms grown in polluted biogas digestate.

The uptake dynamics of two sulfonamide antibiotics, two fluoroquinolone antibiotics, and the anticonvulsant carbamazepine during the cultivation of two species of edible mushrooms (Agaricus subrufescens and A. bisporus) was investigated. None of the antibiotics were accumulated by the mushrooms, while carbamazepine and its transformation product carbamazepine-10,11-epoxide were taken up by A. bisporus fruiting body but only in small amounts (up to 0.76 and 1.85 µg kg-1 dry weight, respectively). The sulfonamides were quickly removed from the mushroom growth substrate, while the recalcitrant fluoroquinolones and carbamazepine were only partially removed. Dissipation half-lives were generally lower for A. subrufescens than A. bisporus, but A. subrufescens was also grown at a slightly higher culture temperature. A. subrufescens also showed a lower uptake of contaminants. Comparison of maximum dietary intake with other common exposure sources showed that these mushrooms can safely be eaten although produced on a polluted substrate, with respect to the investigated compounds.

Anaerobically digested food waste in compost for Agaricus bisporus and Agaricus subrufescens and its effect on mushroom productivity.

BACKGROUND: Source-separated food waste is increasingly being treated by means of hygienisation followed by anaerobic digestion. The fibrous digester residue (digestate) is a potential mushroom substrate, while heat from the biogas can provide steam for the cultivation process. Using bag experiments the present study explored digestate as a full substitute for chicken manure conventionally used in mushroom composts. RESULTS: After mixing, a rapid temperature development in the compost was stimulated by a small amount of chicken manure, as aerobic microbial seeding. Mechanical elimination of lumps was essential for full mycelial colonisation. Three straw digestate composts had Agaricus bisporus mushroom yields above 370 g kg⁻¹ substrate. The optimal compost water content was 600 g kg⁻¹ at inoculation, and high digestate content (up to 500 g kg⁻¹ by dry weight) did not affect yield for this species. High yields of A. subrufescens (200 g kg⁻¹) were related to drier composts of lower digestate content (more straw) and lower pH values at inoculation. CONCLUSION: Digestate successfully substituted chicken manure in straw composts without affecting mushroom yields for both species. There were no clear differences between straw digestate and control composts in terms of mushroom dry matter, size, nitrogen or ash content.