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1.
Clin Endocrinol (Oxf) ; 67(4): 589-97, 2007 Oct.
Article in English | MEDLINE | ID: mdl-17590170

ABSTRACT

OBJECTIVE: In January 1997 we introduced a protocol for the treatment with GH of children with impaired growth after unfractionated total body irradiation (TBI). This study is an evaluation of that protocol. PATIENTS AND METHODS: Between January 1997 and July 2005, 66 patients (48 male) treated for haematological malignancies had at least two years of disease-free survival after TBI-based conditioning for stem cell transplantation (SCT). Stimulated and/or spontaneous GH secretion was decreased in 8 of the 29 patients tested because of impaired growth. Treatment with GH (daily dose 1.3 mg/m2 body surface area) was offered to all 29 patients and initiated in 23 of them (17 male). The main outcome measure was the effect of GH therapy on height standard deviation scores (SDS) after onset of GH therapy, estimated by random-effect modelling with corrections for sex, age at time of SCT and puberty (data analysed on intention-to-treat basis). RESULTS: At time of analysis, median duration of therapy was 3.2 years; median follow-up after start of GH therapy was 4.2 years. The estimated effect of GH therapy, modelled as nonlinear (logit) curve, was +1.1 SD after 5 years. Response to GH therapy did not correlate to GH secretion status. CONCLUSION: GH therapy has a positive effect on height SDS after TBI, irrespective of GH secretion status.


Subject(s)
Growth Disorders/drug therapy , Growth Hormone/blood , Hematopoietic Stem Cell Transplantation , Hormone Replacement Therapy , Human Growth Hormone/therapeutic use , Whole-Body Irradiation , Adolescent , Body Height/drug effects , Carcinoma, Papillary/diagnosis , Child , Female , Follow-Up Studies , Growth Disorders/blood , Growth Disorders/etiology , Growth Hormone/deficiency , Hematologic Neoplasms/therapy , Hormone Replacement Therapy/adverse effects , Humans , Male , Osteosarcoma/diagnosis , Thyroid Neoplasms/diagnosis , Transplantation Conditioning
2.
Clin Endocrinol (Oxf) ; 59(6): 779-87, 2003 Dec.
Article in English | MEDLINE | ID: mdl-14974922

ABSTRACT

BACKGROUND: To investigate in a group of short children born small for gestational age (SGA), the effects of 3 years of GH treatment vs. no treatment on bone age (BA), height and bone mineral density (BMD). Also, to evaluate the influence of the severity of growth retardation at start and the GH dose on the gain in height. PATIENTS AND METHODS: The study design was an open-labelled, controlled multicentre GH study for 3 years. Non-GH-deficient (GHD) children (n = 87) were randomized to either a GH group (n = 61) or an untreated control group (n = 26). In addition, 12 SGA children had GHD (GHD group) and were treated in parallel. Both the GH and the GHD group were treated with a GH dose of 33 microg/kg/day. BMD was evaluated using dual energy X-ray absorptiometry (DEXA). In addition, data of our first GH trial in which short SGA children were treated with a GH dose of 66 microg/kg/day (n = 24) were used for comparison of height gain. RESULTS: In contrast to the control group, the GH group showed a significant increase in height (P < 0.001), as did the parallel GHD group. Bone maturation [delta bone age (BA)/delta calendar age (CA)] increased significantly during the first 2 years of GH treatment but slowed-down thereafter. The 3-year deltaBA/deltaCA ratio correlated significantly with the gain in height (r = 0.6, P < 0.001). At start, mean BMD SDS and mean BMAD SDS were significantly lower than zero. During GH treatment both increased impressively (P < 0.001). The gain in height of children with severe short stature at start (< or = -3.00 SDS), did not differ between those receiving either a GH dose of 33 or 66 microg/kg/day. CONCLUSION: Three years of GH treatment in short children born SGA results in a normalization of height during childhood. Also, bone maturation increased proportionately to the height gain. At start, mean values of BMD and BMAD were significantly reduced but normalized during GH treatment. We did not find an indication to treat very short SGA children (H SDS < or = -3.00) with a higher GH dose. We rather suggest to start GH treatment at an early age in order to achieve a normal height before puberty starts.


Subject(s)
Dwarfism, Pituitary/drug therapy , Human Growth Hormone/therapeutic use , Infant, Small for Gestational Age , Analysis of Variance , Body Height/drug effects , Bone Density/drug effects , Bone Development/drug effects , Child , Child, Preschool , Dwarfism, Pituitary/blood , Female , Humans , Infant, Newborn , Insulin-Like Growth Factor I/analysis , Insulin-Like Growth Factor II/analysis , Male
3.
J Clin Endocrinol Metab ; 86(8): 3877-81, 2001 Aug.
Article in English | MEDLINE | ID: mdl-11502827

ABSTRACT

Autosomal dominantly inherited isolated GH deficiency is caused by mutations of GH-1 that alter the normal structure of GH. We studied 16 familial cases and 1 sporadic case with isolated GH deficiency type II from 1 Dutch and 4 German families by direct sequencing of PCR-amplified genomic DNA and ectopic transcript analysis of lymphocyte mRNA. In addition, the clinical data of the affected individuals were analyzed. Two previously reported mutations and 1 novel splice site mutation in intron III of GH-1 (+1G to C and +1G to A; new, +2T to C) were detected that cause exon 3 skipping. We also discovered a novel G6191 to T missense mutation in exon 4 of GH-1 that changes valine 110, which is highly conserved in mammalian and several nonmammalian GH, to phenylalanine. Splicing of the primary RNA transcript was not affected by this mutation, which is very likely to alter the normal GH structure at the protein level. The onset of growth failure was earlier, and the degree was more severe in affected children with GH-1 splice site mutations compared with those in children with the GH-1 missense mutation. In addition, the severity of the phenotype was variable, even within the same family. The age at diagnosis was between 0.8-9.6 yr (median, 5.1 yr); height at diagnosis was between -2.5 and -8.1 SD score (median, -4.0). Most of the children were lean at diagnosis, with a body mass index ranging from -1.7 to +3.3 SD score (median, -0.5). The 5 affected adults had final heights between -1.8 and -4.5 SD score (median, -3.6), centripetal obesity, and muscular hypotrophy. Before therapy, IGF-I and IGF-binding protein-3 serum levels of all affected children were severely diminished (<<5th percentiles for age). The maximum GH peak in a total of 25 stimulation tests was between 0.1-5.0 microg/liter (median, 0.9), indicating severe GH deficiency. The height of the adenohypophysis studied by magnetic resonance imaging was normal in 2 affected children and mildly decreased in 2 others. Substitution with GH resulted in good catch-up growth in all treated children. Children with severe GH and IGF-I deficiencies, but normal size of the adenohypophysis should be examined for GH-1 splice site and missense mutations. The observed discrepancy between the very homogeneous hormone data proving severe GH and IGF-I deficiencies and the high variability of growth failure even within the same family suggests that the onset and predominance of GH-dependent growth during infancy are individually different and modified by as yet unknown factors.


Subject(s)
Human Growth Hormone/deficiency , Human Growth Hormone/genetics , Hypopituitarism/genetics , Mutation , Age Determination by Skeleton , Amino Acid Sequence , Animals , Child , Child, Preschool , Conserved Sequence , Exons , Female , Genes, Dominant , Growth Hormone/chemistry , Human Growth Hormone/chemistry , Humans , Infant , Male , Mammals , Molecular Sequence Data , Mutation, Missense , Pedigree , Protein Structure, Secondary , Reverse Transcriptase Polymerase Chain Reaction , Sequence Alignment , Sequence Deletion , Sequence Homology, Amino Acid , Vertebrates
4.
J Clin Endocrinol Metab ; 86(6): 2459-64, 2001 Jun.
Article in English | MEDLINE | ID: mdl-11397840

ABSTRACT

GH secretion is regulated by the interaction of GHRH and somatostatin and is released in 10-20 pulses in each 24-h cycle. The exact roles in pulse generation played by somatostatin, GHRH, and the recently isolated GH-releasing peptide, Ghrelin, are not fully elucidated. To investigate the GHRH-mediated GH secretion in human, we investigated pulsatile, entropic, and 24-h rhythmic GH secretion in two young adults (male, 24 yr; female, 23 yr) from a Moroccan family with a novel inactivating defect of the GHRH receptor gene. Data were compared with values in age- and gender-matched controls. Plasma GH concentration were measured by a sensitive immunofluorometric assay, with a detection limit of 0.01 mU/L. All plasma GH concentrations in the female patient were measurable; in the male patient 30 of 145 samples were at or below the detection limit. GH secretion was pulsatile, with 21 and 23 secretory episodes/24 h in the male and female patients, respectively. The fraction of basal to total GH secretion was raised in both patients by 0.18 and 0.15, respectively. The total 24-h GH production rate was greatly diminished; in the male patient it was 6.9 mU/L (normal values for his age, 26--63 mU/L), and in the female patient it was 4.2 mU/L (normal values for her age, 96--390 mU/L). The nyctohemeral plasma GH rhythm was preserved (P < 0.001), with normal acrophases (0430 and 0218 h in the male and female, respectively). Approximate entropy was greatly elevated in both subjects (0.82 in the male and 1.17 in the female; upper normal values for age and gender, 0.24 and 0.59, respectively). Intravenous injection of 50 microg GHRH failed to increase the plasma GH concentration in both patients, but 100 microg GH-releasing peptide-2 elicited a definite increase (male patient, 0.13 to 1.74 mU/L; female patient, 0.29 to 0.87 mU/L). Both patients had a partial empty sella on magnetic resonance imaging scanning. In summary, the present studies in two patients with a profound loss of function mutation of the GHRH receptor favor the view that in the human the timing of GH pulses is primarily supervised by intermittent somatostatin withdrawal, and the amplitude of GH pulses is driven by GHRH. In addition, we infer that effectual GHRH input controls the GH cell mass and the orderliness of the secretory process.


Subject(s)
Human Growth Hormone/metabolism , Mutation/physiology , Receptors, Neuropeptide/genetics , Receptors, Pituitary Hormone-Regulating Hormone/genetics , Adult , Circadian Rhythm , Entropy , Female , Human Growth Hormone/blood , Humans , Male , Oligopeptides/pharmacology , Osmolar Concentration , Pulsatile Flow , Reference Values , Sex Characteristics , Thyrotropin-Releasing Hormone/pharmacology
5.
Trop Geogr Med ; 47(6): 286-8, 1995.
Article in English | MEDLINE | ID: mdl-8650741

ABSTRACT

A retrospective study on the thyroid status of 832 infants, born in Panaga Hospital in Brunei, was conducted. Despite a high degree of ethnic variability, screening for congenital hypothyroidism (CHT) included a fixed T4 and a TSH reference interval, based on a population of Malay infants. We tested their reliability for this heterologous group of infants. New T4 and TSH intervals were determined for each ethnic group and compared, revealing false positive and false negative judgements made during the period of study. Caucasian infants showed significantly higher T4 and TSH serum levels than all other ethnic groups. Regarding T4, most false positive judgements were found among the Malay infants. False negative judgements were detected among the Caucasian female infants. A new reference scheme was recommended, consisting of reference values that are applicable in neonatal thyroid screening of all infants in Panaga Hospital, regardless of their ethnic origin.


Subject(s)
Asian People , Congenital Hypothyroidism , Cultural Diversity , Hypothyroidism/ethnology , Neonatal Screening/methods , White People , Brunei/epidemiology , Female , Humans , Hypothyroidism/blood , Infant, Newborn , Male , Reference Values , Reproducibility of Results , Retrospective Studies , Thyrotropin/blood , Thyroxine/blood
7.
Clin Endocrinol (Oxf) ; 32(5): 603-12, 1990 May.
Article in English | MEDLINE | ID: mdl-2114242

ABSTRACT

A 2-year-old girl presented with gonadotrophin-independent precocious puberty due to ovarian follicles. Central precocious puberty was excluded by several GnRH-tests and overnight LH sampling. There were no signs of McCune-Albright syndrome. An ovarian tumour was excluded by laparotomy and biopsies. Abdominal sonography demonstrated follicles occurring mostly in the left, sometimes in the right, ovary. Immunoglobulin G (IgG) purified from the patient's serum was capable of stimulating DNA synthesis in granulosa cells of rat ovarian segments kept in organ culture. Since FSH had a similar in-vitro action it is hypothesized that this patient's IgG mimics the action of FSH.


Subject(s)
Autoimmune Diseases/complications , Follicular Cyst/complications , Puberty, Precocious/etiology , Animals , Ascorbic Acid/metabolism , Corpus Luteum/metabolism , Cytotoxicity Tests, Immunologic , DNA/biosynthesis , Estradiol/blood , Female , Follicle Stimulating Hormone/blood , Granulosa Cells/metabolism , Humans , Immunoglobulin G/immunology , Infant , Leydig Cells/immunology , Luteinizing Hormone/blood , Male , Puberty, Precocious/immunology , Rats , Rats, Inbred Strains
8.
Tijdschr Kindergeneeskd ; 54(5): 151-4, 1986 Oct.
Article in Dutch | MEDLINE | ID: mdl-3798443

ABSTRACT

We present an eleven years old girl with an enlarged cervical lymph node. This turned out to be metastasis of a papillary thyroid carcinoma. The treatment consisted of surgical intervention and radioactive iodine orally. Even in childhood thyroid carcinoma should be included in the differential diagnosis of a persisting swelling in the neck.


Subject(s)
Carcinoma, Papillary/diagnosis , Thyroid Neoplasms/diagnosis , Carcinoma, Papillary/pathology , Carcinoma, Papillary/therapy , Child , Combined Modality Therapy , Female , Humans , Lymphatic Metastasis , Thyroid Neoplasms/pathology , Thyroid Neoplasms/therapy
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