Comparative Life Cycle Assessment of Chemical and Biocatalytic 2'3'-Cyclic GMP-AMP Synthesis.

Life cycle assessments (LCAs) can provide insights into the environmental impact of production processes. In this study, a comparative LCA was performed for the synthesis of 2'3'-cyclic GMP-AMP (2'3'-cGAMP) in an early development stage. The cyclic dinucleotide (CDN) is of interest for pharmaceutical applications such as cancer immunotherapy. CDNs can be synthesized either by enzymes or chemical catalysis. It is not known which of the routes is more sustainable as both routes have their advantages and disadvantages, such as a poor yield for the chemical synthesis and low titers for the biocatalytic synthesis. The synthesis routes were compared for the production of 200 g 2'3'-cGAMP based on laboratory data to assess the environmental impacts. The biocatalytic synthesis turned out to be superior to the chemical synthesis in all considered categories by at least one magnitude, for example, a global warming potential of 3055.6 kg CO2 equiv. for the enzymatic route and 56454.0 kg CO2 equiv. for the chemical synthesis, which is 18 times higher. This study demonstrates the value of assessment at an early development stage, when the choice between different routes is still possible.

Assessment of the Chemical State of Bottom Sediments in the Eutrophied Dam Reservoir in Poland.

The aim of the presented research was to examine the concentration of biogenic compounds and heavy metals in the bottom sediments of the Sulejów Reservoir (Central Poland) from October 2018. Based on the obtained research results, maps of the spatial distribution were prepared. The following parameters were analyzed: total phosphorus (TP), total Kjeldahl nitrogen, total organic carbon (TOC), ratio of total organic carbon to nitrogen (C:N), organic matter content as well as Cd, Cr and Pb concentrations. The sediments were collected at 28 sampling sites, covering the whole area of the reservoir. The differences in the content of individual biogenic compounds result from the composition of the debris applied by the supplying rivers, as well as the content of this elements in the water, long retention time (40 days) and depth from which the tested sediments were taken. The distribution of examined compounds was largely influenced by the agricultural activity in the studied area, as well as the presence of ports and recreational points. Based on the measurements, the highest amounts of biogenic components deposit in sediments of deep parts in slow-flowing waters, in stagnation zones, areas adjacent to arable land, and the sites where fine-size fractions prevail in the deposited material. Biogenic compounds in sediments of the Sulejów Reservoir showed a pattern of gradual increase along the reservoir from lower values in the back-water part. A similar relationship is visible for heavy metals. Referring to the ecotoxicological criteria, it can be stated that bottom sediments from the Sulejów Reservoir collected in 2018 are not toxicologically contaminated in terms of cadmium, lead and chromium content.

Continuous and Periodic Monitoring System of Surface Water Quality of an Impounding Reservoir: Sulejow Reservoir, Poland.

The paper presents results of water quality monitoring conducted within the frame of the MONSUL project. The main goal was to analyse and assess the impact of factors determining the ecological status of a dam reservoir on the basis of the Sulejow Reservoir located in Central Poland. The project implementation plan based on comprehensive research-based monitoring covered the following parameters characterising the ecological potential of the reservoir: water temperature, pH, oxygen concentration, chlorophyll "a" and blue-green algae, concentration of ammonium ion, nitrate nitrogen phosphates as well as total organic carbon, chemical oxygen demand and biochemical oxygen demand. The parameters were measured with a mobile and stationary monitoring system and supplemented by an off-line analysis of water samples in the laboratory. The study was carried out during two seasons: May­October 2015 and April­November 2016; the results were analysed also with regard to the weather conditions. Despite the similar temperatures of water and air in the analysed seasons, significant differences were observed for atmospheric precipitation; 2015 was a dry year, and the climatic water balance for the analysed area was negative, which caused limited surface runoff and decreased the concentrations of nutrient in the reservoir waters. Data from continuous monitoring, supplemented with the results of laboratory measurements, indicated that the values of TOC (Total Organic Carbon) and COD (Chemical Oxygen Demand) parameters were within the purity class I; exceedances refer to the BOD (Biochemical Oxygen Demand) value, which confirmed the presence of biodegradable organic compounds in the reservoir waters. The values of chlorophyll "a" and the presence of algae during the vegetation season testify to eutrophication of the Sulejow Reservoir.

Evisceration and intestine damage as a rare form of suicide in forensic and psychiatric practice - description of two cases.

Suicide is a major public health problem around the world. Case reports include extreme suicidal self-inflicted injuries, in which extensive damage to the abdominal wall associated with injury or even excision of fragments of the intestine and its fragmentation are present. These cases usually give rise to doubts of investigators as to the course of the incident, the circumstances of death and the possibility of participation of other people. At the same time they are interesting from the medico-legal and psychiatric perspective. The aim of this study is the presentation of two extremely rare cases of suicides through evisceration and intestinal injury from the clinical and opinioning practice of the authors, one of which ended with survival and the second one with death. Regardless of the final result of the suicide attempt (death or survival), good practice of the investigative teams in such cases should include a detailed examination of the place of the suicide attempt, obtaining opinion of an expert in the field of forensic medicine with full post-mortem diagnosis, and in-depth forensic psychological and psychiatric analysis of, among others, lifeline, mental state and suicidal motivation (so-called psychological autopsy).