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In vitro sulfation of N-acetyllactosaminide by soluble recombinant human beta-Gal-3'-sulfotransferase.
Greimel, P; Jabs, Sabrina; Storch, Stefan; Cherif, Slim; Honke, Koichi; Braulke, Thomas; Thiem, Joachim.
Carbohydr Res ; 341(7): 918-24, 2006 May 22.
Article in English | MEDLINE | ID: mdl-16516177

ABSTRACT

Membrane-bound beta-Gal-3'-sulfotransferase (GP3ST) was expressed and used for in vitro sulfation of Tamm-Horsfall glycoprotein. Further, the regioselective transfer of sulfate to an N-acetyllactosamine derivative could be realised with soluble chimeric GP3ST, also in combination with Lac transglycosylation by means of beta-galactosidase. Two alternative straightforward chemical syntheses for the target compound could be elaborated.


Subject(s)
Mucoproteins/metabolism , Oligosaccharides/metabolism , Sulfotransferases/metabolism , Animals , Binding Sites , Cell Line , Cricetinae , Glycoside Hydrolases/metabolism , Glycosylation , Humans , In Vitro Techniques , Protein Binding , Protein Conformation , Protein Structure, Tertiary , Recombinant Fusion Proteins/chemistry , Recombinant Fusion Proteins/metabolism , Solubility , Staphylococcal Protein A/chemistry , Sulfates/metabolism , Sulfotransferases/chemistry , Sulfotransferases/genetics , Sulfotransferases/isolation & purification , Sulfotransferases/urine , Uromodulin
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