Measurement of dye diffusion in scattering tissue phantoms using dual-wavelength low-coherence interferometry.

We demonstrate low-coherence interferometry (LCI) for dye diffusion measurements in scattering tissue phantoms. The diffusion coefficient of a phthalocyanine dye in 1.5% agar gel containing scattering Intralipid was measured using a dual-wavelength interfero-meter. One wavelength was matched to the absorption peak of the dye at 675 nm. The other, 805 nm, was not affected by the dye, and was used to correct for varying sample scattering as a function of depth, assuming a constant ratio between scattering at the two wavelengths. The same wavelength dependence of scattering is assumed for the entire sample, but no a priori knowledge about the amount of scattering is needed. The dye diffusion coefficient was estimated by fitting a mathematical model of the interferometer signal to the measured LCI envelope. We compare results obtained using both a constant-scattering and a depth-resolved-scattering approach to determine the sample scattering. The presented method provides robust estimation of the diffusion coefficient when spatial resolution in determining the depth-resolved scattering is varied. Results indicate that the method is valid for samples having continuous spatial variations in the scattering coefficient over lengths as short as the coherence length of the probing light. The method allows in situ characterization of diffusion in scattering media.

Functional imaging of dye concentration in tissue phantoms by spectroscopic optical coherence tomography.

We present functional imaging of the concentration of a photodynamic therapy (PDT)-related dye in scattering tissue phantoms based on spatially resolved measurements of optical properties through spectroscopic optical coherence tomography (OCT). Expressions for the OCT signal are developed, enabling estimation of depth-resolved sample optical properties. Based on these expressions, we discuss speckle statistics and speckle correlations of the OCT signal. Speckle noise reduction is performed by spatial filtering and is used to improve accuracy in the estimated optical properties at the expense of spatial resolution. An analytic expression for the precision in the estimated optical properties is derived. This expression shows that axial filtering, and thereby a reduction of axial resolution, gives a larger improvement in precision compared to the same filtering and reduction in the transversal resolution. It also shows that imaging with a shorter coherence length, or a larger numerical aperture, improves precision when the filter length determines the spatial resolution. Good agreement is obtained between experimentally determined and theoretically predicted variance in the estimated attenuation coefficients and dye concentration. Finally, we present guidelines for spectroscopic OCT systems for concentration imaging and discuss application of the method to more realistic phantoms and tissue.

Measurement of dye diffusion in agar gel by use of low-coherence interferometry.

We demonstrate low-coherence interferometry for diffusion measurements. We have measured the diffusion coefficient of a phthalocyanine dye in 1.5% agar gel with a two-wavelength interferometer; one wavelength was matched to the absorption peak of the dye at 675 nm, while the other, 805 nm, was not affected by the dye. The diffusion coefficient of the dye was found by fitting a mathematical model for the interferometer signal to the measured low-coherence interferometry amplitude. A 95% confidence interval for the diffusion coefficient was found to be D = (2.5 +/- 0.2) x 10(-10) m2/s. The influence of speckle averaging and experiment time on the determination of the diffusion coefficient has been studied. The presented technique allows in situ characterization of diffusion in semitransparent media.