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1.
ACS Appl Mater Interfaces ; 12(19): 21322-21329, 2020 May 13.
Article in English | MEDLINE | ID: mdl-32259428

ABSTRACT

Much recent effort has been directed toward the development of novel antimicrobial materials able to defeat new and antibiotic resistant pathogens. In this report, we study the efficacy of cationic poly(phenylene ethynylene), polythiophene, and oligo(phenylene ethynylene) electrolytes against laboratory strains of Pseudomonas aeruginosa, Staphylococcus aureus and Staphylococcus epidermidis. The focus of the study is to quantitatively evaluate the speed and extent of dark and light-activated antimicrobial activity. Using cell plating with serial dilutions, we determined that planktonic bacteria suspensions exposed to the antimicrobials (at 10 µg/mL) result in several log kills at 10 min both in the dark and under UV irradiation (360 nm) for all eight synthetic antimicrobials. However, there are significant differences in the ease of killing the different pathogens. In most trials, there is significantly greater killing under light-irradiation, indicating these materials may be used as versatile disinfectants.


Subject(s)
Anti-Bacterial Agents/pharmacology , Disinfectants/pharmacology , Polymers/pharmacology , Thiophenes/pharmacology , Anti-Bacterial Agents/radiation effects , Darkness , Disinfectants/radiation effects , Microbial Sensitivity Tests , Polymers/radiation effects , Pseudomonas aeruginosa/drug effects , Staphylococcus aureus/drug effects , Staphylococcus epidermidis/drug effects , Thiophenes/radiation effects , Ultraviolet Rays
2.
Photochem Photobiol ; 94(6): 1116-1123, 2018 11.
Article in English | MEDLINE | ID: mdl-30222200

ABSTRACT

Cationic polythiophenes have been shown to be potent antimicrobial compounds due to their ability to absorb visible light and sensitize the production of reactive oxygen species (ROS) as well as their ability to selectively associate with and damage negatively charged cell envelopes. This study demonstrates the ability of differentially sized imidazolium- and tertiary amine-functionalized poly(3-hexylthiophene) (P3HT) to inactivate Gram-negative Escherichia coli and Gram-positive Bacillus atrophaeus under photolysis and dark conditions. Flow cytometry viability assays are used to quantify cell death. Each compound shows high levels of killing at both 1 and 10 µg mL-1 polymer concentrations for each microbial species after photoactivation as well as high levels of dark inactivation in many cases. Tertiary amine-functionalized P3HT is shown to have different killing patterns, shown by transmission electron microscopy, compared to the imidazolium-functionalized derivatives.


Subject(s)
Anti-Bacterial Agents/chemistry , Bacillus/drug effects , Escherichia coli/drug effects , Photosensitizing Agents/chemistry , Thiophenes/chemistry , Amines/chemistry , Anti-Bacterial Agents/chemical synthesis , Anti-Bacterial Agents/pharmacology , Bacillus/chemistry , Bacillus/growth & development , Cations , Escherichia coli/chemistry , Escherichia coli/growth & development , Imidazoles/chemistry , Light , Microbial Sensitivity Tests , Microbial Viability/drug effects , Photolysis , Photosensitizing Agents/chemical synthesis , Photosensitizing Agents/pharmacology , Polymerization , Reactive Oxygen Species/agonists , Reactive Oxygen Species/metabolism , Structure-Activity Relationship , Thiophenes/chemical synthesis , Thiophenes/pharmacology
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