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1.
PLoS One ; 15(5): e0226453, 2020.
Article in English | MEDLINE | ID: mdl-32379825

ABSTRACT

Membrane transporters carry key metabolites across the cell membrane and, from a resource standpoint, are hypothesized to be produced when necessary. The expression of membrane transporters in metabolic pathways is often upregulated by the transporter substrate. In E. coli, such systems include for example the lacY, araFGH, and xylFGH genes, which encode for lactose, arabinose, and xylose transporters, respectively. As a case study of a minimal system, we build a generalizable physical model of the xapABR genetic circuit, which features a regulatory feedback loop via membrane transport (positive feedback) and enzymatic degradation (negative feedback) of an inducer. Dynamical systems analysis and stochastic simulations show that the membrane transport makes the model system bistable in certain parameter regimes. Thus, it serves as a genetic "on-off" switch, enabling the cell to only produce a set of metabolic enzymes when the corresponding metabolite is present in large amounts. We find that the negative feedback from the degradation enzyme does not significantly disturb the positive feedback from the membrane transporter. We investigate hysteresis in the switching and discuss the role of cooperativity and multiple binding sites in the model circuit. Fundamentally, this work explores how a stable genetic switch for a set of enzymes is obtained from transcriptional auto-activation of a membrane transporter through its substrate.


Subject(s)
Adaptation, Physiological/genetics , Escherichia coli/genetics , Escherichia coli/metabolism , Gene Regulatory Networks , Genes, Switch , Models, Biological , Binding Sites , Biological Transport/genetics , Escherichia coli Proteins/genetics , Escherichia coli Proteins/metabolism , Feedback, Physiological , Gene Expression Regulation, Bacterial , Membrane Transport Proteins/genetics , Membrane Transport Proteins/metabolism , Pentosyltransferases/genetics , Pentosyltransferases/metabolism , Promoter Regions, Genetic , RNA, Messenger/metabolism , Ribonucleosides/metabolism , Stochastic Processes , Trans-Activators/genetics , Trans-Activators/metabolism , Transcription, Genetic , Xanthines
2.
Phys Rev E ; 99(6-1): 062306, 2019 Jun.
Article in English | MEDLINE | ID: mdl-31330579

ABSTRACT

We study a simple model in which the growth of a network is determined by the location of one or more random walkers. Depending on walker motility rate, the model generates a spectrum of structures situated between well-known limiting cases. We demonstrate that the average degree observed by a walker is a function of its motility rate. Modulating the extent to which the location of node attachment is determined by the walker as opposed to random selection is akin to scaling the speed of the walker and generates new limiting behavior. The model raises questions about energetic and computational resource requirements in a physical instantiation.

3.
Dev Cell ; 43(4): 480-492.e6, 2017 11 20.
Article in English | MEDLINE | ID: mdl-29107560

ABSTRACT

Under conditions of homeostasis, dynamic changes in the length of individual adherens junctions (AJs) provide epithelia with the fluidity required to maintain tissue integrity in the face of intrinsic and extrinsic forces. While the contribution of AJ remodeling to developmental morphogenesis has been intensively studied, less is known about AJ dynamics in other circumstances. Here, we study AJ dynamics in an epithelium that undergoes a gradual increase in packing order, without concomitant large-scale changes in tissue size or shape. We find that neighbor exchange events are driven by stochastic fluctuations in junction length, regulated in part by junctional actomyosin. In this context, the developmental increase of isotropic junctional actomyosin reduces the rate of neighbor exchange, contributing to tissue order. We propose a model in which the local variance in tension between junctions determines whether actomyosin-based forces will inhibit or drive the topological transitions that either refine or deform a tissue.


Subject(s)
Adherens Junctions/physiology , Drosophila Proteins/metabolism , Drosophila melanogaster/metabolism , Epithelium/metabolism , Myosin Type II/metabolism , Actomyosin/metabolism , Animals , Cadherins/metabolism
4.
Article in English | MEDLINE | ID: mdl-26651721

ABSTRACT

While biological studies suggest that motility of cells is involved in cell segregation, few computational models have investigated this mechanism. We apply a simple Schelling model, modified to reflect biological conditions, demonstrating how differences in cell motility arising exclusively from differences in the composition of the local environment can be sufficient to drive segregation. The work presented here demonstrates that the segregation behavior observed in the original Schelling model is robust to a relaxation of the requirement for global information and that the Schelling model may yield insight in the context of biological systems. In the model, the time course of cell segregation follows a power law in accord with experimental observations and previous work.


Subject(s)
Cell Separation , Models, Biological
5.
Interface Focus ; 4(6): 20140013, 2014 Dec 06.
Article in English | MEDLINE | ID: mdl-25485079

ABSTRACT

Our current understanding of cell sorting relies on physical difference, either in the interfacial properties or motile force, between cell types. But is such asymmetry a prerequisite for cell sorting? We test this using a minimal model in which the two cell populations are identical with respect to their physical properties and differences in motility arise solely from how cells interact with their surroundings. The model resembles the Schelling model used in social sciences to study segregation phenomena at the scale of societies. Our results demonstrate that segregation can emerge solely from cell motility being a dynamic property that changes in response to the local environment of the cell, but that additional mechanisms are necessary to reproduce the envelopment behaviour observed in vitro. The time course of segregation follows a power law, in agreement with the scaling reported from experiment and in other models of motility-driven segregation.

6.
PLoS One ; 9(2): e87917, 2014.
Article in English | MEDLINE | ID: mdl-24551071

ABSTRACT

The asymmetric distribution of damaged cellular components has been observed in species ranging from fission yeast to humans. To study the potential advantages of damage segregation, we have developed a mathematical model describing ageing mammalian tissue, that is, a multicellular system of somatic cells that do not rejuvenate at cell division. To illustrate the applicability of the model, we specifically consider damage incurred by mutations to mitochondrial DNA, which are thought to be implicated in the mammalian ageing process. We show analytically that the asymmetric distribution of damaged cellular components reduces the overall damage level and increases the longevity of the cell population. Motivated by the experimental reports of damage segregation in human embryonic stem cells, dividing symmetrically with respect to cell-fate, we extend the model to consider spatially structured systems of cells. Imposing spatial structure reduces, but does not eliminate, the advantage of asymmetric division over symmetric division. The results suggest that damage partitioning could be a common strategy for reducing the accumulation of damage in a wider range of cell types than previously thought.


Subject(s)
Aging/genetics , Cell Division , DNA, Mitochondrial/genetics , Models, Statistical , Animals , Apoptosis/genetics , DNA Damage , Humans , Longevity , Mutation
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